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Production and Characterization of Partially Purified Chitosanase Enzyme of Bacillus cereus A4/B4 Isolated from Biowaste Soil Samples:Bioactive Chitooligosaccharide


Affiliations
1 Department of Microbiology, Maharaja Co Education Arts & Science College, Perundurai, India
2 Division of Microbiology and immunology, Pondicherry centre for biological sciences, Pondicherry, India
3 Department of Nanotechnology, Bharathiar University, Coimbatore, India
4 PSG College of Arts & Science, Coimbatore, India
 

Microbial chitosanase has received the attention of producing chitooligosaccharides. In this study, Chitooligosaccharide was developed by chitosanase enzyme hydrolysis and evaluated for its bioactivity. Chitosan degrading Bacillus cereus was isolated from biowaste sample and its extracellular enzyme chitosanase was characterized. The chitosanase enzyme production was induced by chitosan substrate and the enzyme production reached the maximum in 72 hours. The optimum pH and temperature for the production was 6.5 and 30°C respectively. The culture medium with 1.0 to 1.5% of substrate level with xylose served as significantly for enzyme production. The molecular weight of the enzyme 43KDa was determined by SDS-PAGE. The chitooligosaccharide produced by chitosanase hydrolysis inhibited the growth of the pathogens and showed MIC50 as 0.2 μg/ml against all the test pathogens and has proved to be a potential antimicrobial agent against skin and other infections.

Keywords

Bacillus Species, Bioproduction, Chitosanase, Chitooligosaccharides, Microbial Enzymes.
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  • Production and Characterization of Partially Purified Chitosanase Enzyme of Bacillus cereus A4/B4 Isolated from Biowaste Soil Samples:Bioactive Chitooligosaccharide

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Authors

P. Vanathi
Department of Microbiology, Maharaja Co Education Arts & Science College, Perundurai, India
S. Fernandez
Department of Microbiology, Maharaja Co Education Arts & Science College, Perundurai, India
M. Shanmugapriya
Department of Microbiology, Maharaja Co Education Arts & Science College, Perundurai, India
T. S. Riyamol
Department of Microbiology, Maharaja Co Education Arts & Science College, Perundurai, India
V. Loganathan
Department of Microbiology, Maharaja Co Education Arts & Science College, Perundurai, India
V. I. Hairul-Islam
Division of Microbiology and immunology, Pondicherry centre for biological sciences, Pondicherry, India
P. Premasudha
Department of Nanotechnology, Bharathiar University, Coimbatore, India
R. Rajendran
PSG College of Arts & Science, Coimbatore, India

Abstract


Microbial chitosanase has received the attention of producing chitooligosaccharides. In this study, Chitooligosaccharide was developed by chitosanase enzyme hydrolysis and evaluated for its bioactivity. Chitosan degrading Bacillus cereus was isolated from biowaste sample and its extracellular enzyme chitosanase was characterized. The chitosanase enzyme production was induced by chitosan substrate and the enzyme production reached the maximum in 72 hours. The optimum pH and temperature for the production was 6.5 and 30°C respectively. The culture medium with 1.0 to 1.5% of substrate level with xylose served as significantly for enzyme production. The molecular weight of the enzyme 43KDa was determined by SDS-PAGE. The chitooligosaccharide produced by chitosanase hydrolysis inhibited the growth of the pathogens and showed MIC50 as 0.2 μg/ml against all the test pathogens and has proved to be a potential antimicrobial agent against skin and other infections.

Keywords


Bacillus Species, Bioproduction, Chitosanase, Chitooligosaccharides, Microbial Enzymes.

References





DOI: https://doi.org/10.15613/fijrfn%2F2015%2Fv2i2%2F96643