Indian Journal of Science and Technology

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TiO2 nano-photosensitizer particles less than 10nm have been synthesized and characterized. They were prepared by hydrolysing Titanium (IV) isopropoxide in presence of ultrasonic waves and further characterized by absorption and fluorescence spectroscopy, XRD, FTIR, TGA, DSC, SEM, and HRTEM techniques. The XRD patterns revealed exclusive formation of anatase without contamination by rutile form. High resolution transmission electron microscopic measurements revealed their size below 10nm. The photohemolysis induced by TiO2 NPs reveals that the percent hemolysis increased with the increase in concentration and light dose. The study of effect of scavengers, GSH and NaN3 showed the formation of considerable amount of superoxide anion and singlet oxygen that caused cell death. The mechanism has been discussed. TiO2 nano-photosensitizer being non-toxic, serves as proper substitute for the classical photosensitizers (organic dyes).
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