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The Synthesis and Characterization of Titanium Dioxide Nanoparticles as Potential Photosensitizer in Photodynamic Therapy


Affiliations
1 Department of Chemistry, Queen Mary’s College, Chennai, India
2 Department of Physics, Department of Chemistry- Anna University, Chennai, India
3 Department of Chemistry- Anna University, Chennai, India
4 Department of Chemistry, Adhiparasakthi College of Science, Kalavai, India
 

TiO2 nano-photosensitizer particles less than 10nm have been synthesized and characterized. They were prepared by hydrolysing Titanium (IV) isopropoxide in presence of ultrasonic waves and further characterized by absorption and fluorescence spectroscopy, XRD, FTIR, TGA, DSC, SEM, and HRTEM techniques. The XRD patterns revealed exclusive formation of anatase without contamination by rutile form. High resolution transmission electron microscopic measurements revealed their size below 10nm. The photohemolysis induced by TiO2 NPs reveals that the percent hemolysis increased with the increase in concentration and light dose. The study of effect of scavengers, GSH and NaN3 showed the formation of considerable amount of superoxide anion and singlet oxygen that caused cell death. The mechanism has been discussed. TiO2 nano-photosensitizer being non-toxic, serves as proper substitute for the classical photosensitizers (organic dyes).
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  • The Synthesis and Characterization of Titanium Dioxide Nanoparticles as Potential Photosensitizer in Photodynamic Therapy

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Authors

Karunakaran Sulochana Meena
Department of Chemistry, Queen Mary’s College, Chennai, India
Prakasa Rao Aruna
Department of Physics, Department of Chemistry- Anna University, Chennai, India
Velayutham Murugesan
Department of Chemistry- Anna University, Chennai, India
Thyagarajan Venkataraman
Department of Chemistry, Adhiparasakthi College of Science, Kalavai, India

Abstract


TiO2 nano-photosensitizer particles less than 10nm have been synthesized and characterized. They were prepared by hydrolysing Titanium (IV) isopropoxide in presence of ultrasonic waves and further characterized by absorption and fluorescence spectroscopy, XRD, FTIR, TGA, DSC, SEM, and HRTEM techniques. The XRD patterns revealed exclusive formation of anatase without contamination by rutile form. High resolution transmission electron microscopic measurements revealed their size below 10nm. The photohemolysis induced by TiO2 NPs reveals that the percent hemolysis increased with the increase in concentration and light dose. The study of effect of scavengers, GSH and NaN3 showed the formation of considerable amount of superoxide anion and singlet oxygen that caused cell death. The mechanism has been discussed. TiO2 nano-photosensitizer being non-toxic, serves as proper substitute for the classical photosensitizers (organic dyes).

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DOI: https://doi.org/10.17485/ijst%2F2007%2Fv1i2%2F29203