Indian Journal of Science and Technology

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Production, Optimization and Characterization of Poly[R]Hydroxyalkanoate from Enterobacter sp SU16


Affiliations
1 Department of Chemical Engineering, School of Bio and Chemical Engineering, Sathyabama University, India
2 Centre for Waste Management, Sathyabama University, India
3 Department of Biotechnology, School of Bio and Chemical Engineering, Sathyabama University, India
 

Objectives: To produce, optimize and characterize poly[R]hydroxyalkanoate (PHA) from Enterobacter sp SU16. Methods: isolation of Enterobacter sp SU16 from dairy waste. Optimization of PHA production was done. Extraction of PHA was done by non-solvent precipitation method. Presence of PHA was confirmed by UV Spectrometry and TLC. Characterization of PHA was done by FTIR and GCMS analysis. Findings: Optimal PHA production was observed at 48h incubation at 35°C in 3% glucose containing medium at pH 8. PHA was found to with 3-hydroxydodecanoic acid monomers. Novelty: Enterobacter sp SU16 to accumulate polyhydroxydecanoate (PHD) monomers.

Keywords

FTIR analysis, GC-MS analysis, Enterobacter sp SU16, poly[R]hydroxyalkanoate - PHA.
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  • Production, Optimization and Characterization of Poly[R]Hydroxyalkanoate from Enterobacter sp SU16

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Authors

Senthilkumar Pachiyappan
Department of Chemical Engineering, School of Bio and Chemical Engineering, Sathyabama University, India
Dawn SS
Centre for Waste Management, Sathyabama University, India
Antony V Samrot
Department of Biotechnology, School of Bio and Chemical Engineering, Sathyabama University, India
Narendra Kumar G
Department of Biotechnology, School of Bio and Chemical Engineering, Sathyabama University, India
Aashrit Raj D
Department of Chemical Engineering, School of Bio and Chemical Engineering, Sathyabama University, India

Abstract


Objectives: To produce, optimize and characterize poly[R]hydroxyalkanoate (PHA) from Enterobacter sp SU16. Methods: isolation of Enterobacter sp SU16 from dairy waste. Optimization of PHA production was done. Extraction of PHA was done by non-solvent precipitation method. Presence of PHA was confirmed by UV Spectrometry and TLC. Characterization of PHA was done by FTIR and GCMS analysis. Findings: Optimal PHA production was observed at 48h incubation at 35°C in 3% glucose containing medium at pH 8. PHA was found to with 3-hydroxydodecanoic acid monomers. Novelty: Enterobacter sp SU16 to accumulate polyhydroxydecanoate (PHD) monomers.

Keywords


FTIR analysis, GC-MS analysis, Enterobacter sp SU16, poly[R]hydroxyalkanoate - PHA.



DOI: https://doi.org/10.17485/ijst%2F2016%2Fv9i45%2F128732