Journal of Pharmaceutical Research

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Phyllanthus Reticulates Inhibits Isoprenaline Induced Oxidative Stress in Rat's Heart


Affiliations
1 Department of Pharmacology, Krupanidhi College of Pharmacy, 12/1, Chikkabellandur, Carmelram Post, Bangalore-560 035, India
2 Department of Pharmaceutical Chemistry, Krupanidhi College of Pharmacy, 12/1, Chikkabellandur, Carmelram Post, Bangalore-560 035, India
 

The objective of the present study was to carryout cardioprotective effect of Phyllanthus reticulatus leaves extract (PRLE) against isoprenaline (ISP) induced myocardial dysfunction in rats. Rats were administered low (100 mg/kg) and high (500 mg/kg) doses of PRLE orally for three weeks, while propranolol (PRO) was given for one week. At the end of treatment, isoprenaline (150 mg/kg, s.c) was administered for two consecutive days. LDH and CK-MB were estimated in serum as well as in heart tissue homogenate (HTH) apart from SOD, Catalase and TBARS determinations in HTH. Both high and low doses of PRLE as well as PRO were found to decrease the LDH and CK-MB activities in serum and increase in HTH. SOD and Catalase activities were also found to rise significantly in all treated groups. The estimation of TBARS demonstrates an inclination in free radical formation in ISO group that was ameliorated by prophylactic treatment of both high and low dose of PRLE and PRO. The best results were found in group subjected to high dose of PRLE. The results of the study revealed efficacies of both high and low doses of PRLE and PRO on isoprenaline induced myocardial damage in rats.

Keywords

Phyllanthus reticulatus, Antioxidants, Cardioprotection, Isoprenaline.
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  • Phyllanthus Reticulates Inhibits Isoprenaline Induced Oxidative Stress in Rat's Heart

Abstract Views: 154  |  PDF Views: 80

Authors

D. D. Mistry
Department of Pharmacology, Krupanidhi College of Pharmacy, 12/1, Chikkabellandur, Carmelram Post, Bangalore-560 035, India
R. K. Rawry
Department of Pharmaceutical Chemistry, Krupanidhi College of Pharmacy, 12/1, Chikkabellandur, Carmelram Post, Bangalore-560 035, India
S. M. Asdaq
Department of Pharmacology, Krupanidhi College of Pharmacy, 12/1, Chikkabellandur, Carmelram Post, Bangalore-560 035, India
N. Naira
Department of Pharmaceutical Chemistry, Krupanidhi College of Pharmacy, 12/1, Chikkabellandur, Carmelram Post, Bangalore-560 035, India

Abstract


The objective of the present study was to carryout cardioprotective effect of Phyllanthus reticulatus leaves extract (PRLE) against isoprenaline (ISP) induced myocardial dysfunction in rats. Rats were administered low (100 mg/kg) and high (500 mg/kg) doses of PRLE orally for three weeks, while propranolol (PRO) was given for one week. At the end of treatment, isoprenaline (150 mg/kg, s.c) was administered for two consecutive days. LDH and CK-MB were estimated in serum as well as in heart tissue homogenate (HTH) apart from SOD, Catalase and TBARS determinations in HTH. Both high and low doses of PRLE as well as PRO were found to decrease the LDH and CK-MB activities in serum and increase in HTH. SOD and Catalase activities were also found to rise significantly in all treated groups. The estimation of TBARS demonstrates an inclination in free radical formation in ISO group that was ameliorated by prophylactic treatment of both high and low dose of PRLE and PRO. The best results were found in group subjected to high dose of PRLE. The results of the study revealed efficacies of both high and low doses of PRLE and PRO on isoprenaline induced myocardial damage in rats.

Keywords


Phyllanthus reticulatus, Antioxidants, Cardioprotection, Isoprenaline.