Open Access Open Access  Restricted Access Subscription Access

A Validated RP HPLC Method for the Determination of Moprolol in Bulk and Tablet Dosage Form


Affiliations
1 Department of Pharmaceutical Analysis, Srinivas College of Pharmacy, Valachil, Mangalore, Karnataka - 574 143, India
2 Department of Pharmaceutics, Srinivas College of Pharmacy, Valachil, Mangalore, Karnataka-574143, India
3 Department of Pharmaceutical Analysis, Adhiparasakthi College of Pharmacy, Melmaruvathur, Kancheepuram, Tamilnadu-603319, India
 

A validated reverse phase high performance liquid chromatographic method (RP-HPLC) has been developed for the estimation of moprolol in the pharmaceutical preparation using RPC18 column. The mobile phase (Acetonitrile:Methanol:Water) was pumped at a flow rate of 1ml/min in the ratio of 15:50:35 and the eluents were monitored at 274nm at 30°C. The intra and interday precision was found to be less than 2% showing high precision of the assay method. The % recovery of the method was more than 99% and RSD did not exceed 2% indicating high degree of accuracy of the proposed HPLC method. The %RSD for the robustness testing was also less than 2%.The proposed HPLC method can be used for the estimation of moprolol in tablet dosage forms.

Keywords

Moprolol, RP-HPLC, Validation, Recovery, Tablet Dosage.
User
Notifications
Font Size


Abstract Views: 183

PDF Views: 78




  • A Validated RP HPLC Method for the Determination of Moprolol in Bulk and Tablet Dosage Form

Abstract Views: 183  |  PDF Views: 78

Authors

Rekha Rajeevkumar
Department of Pharmaceutical Analysis, Srinivas College of Pharmacy, Valachil, Mangalore, Karnataka - 574 143, India
P. Rajeevkumar
Department of Pharmaceutics, Srinivas College of Pharmacy, Valachil, Mangalore, Karnataka-574143, India
T. Vetrichelvan
Department of Pharmaceutical Analysis, Adhiparasakthi College of Pharmacy, Melmaruvathur, Kancheepuram, Tamilnadu-603319, India
D. Nagavalli
Department of Pharmaceutical Analysis, Adhiparasakthi College of Pharmacy, Melmaruvathur, Kancheepuram, Tamilnadu-603319, India

Abstract


A validated reverse phase high performance liquid chromatographic method (RP-HPLC) has been developed for the estimation of moprolol in the pharmaceutical preparation using RPC18 column. The mobile phase (Acetonitrile:Methanol:Water) was pumped at a flow rate of 1ml/min in the ratio of 15:50:35 and the eluents were monitored at 274nm at 30°C. The intra and interday precision was found to be less than 2% showing high precision of the assay method. The % recovery of the method was more than 99% and RSD did not exceed 2% indicating high degree of accuracy of the proposed HPLC method. The %RSD for the robustness testing was also less than 2%.The proposed HPLC method can be used for the estimation of moprolol in tablet dosage forms.

Keywords


Moprolol, RP-HPLC, Validation, Recovery, Tablet Dosage.