Toxicology International (Formerly Indian Journal of Toxicology)

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Effect of Imidacloprid on Histopathological Alterations of Brain, Gills and Eyes in hatchling carp (Cyprinus carpio L.)


Affiliations
1 Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana, India
     

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Imidacloprid, a neonicotinoid pesticide is used globally to control insect pest in rural and urban area where it enters into and contaminate the aquatic ecosystem. The present study was designed to study the toxic impacts of imidacloprid on developing hatchlings of Cyprinus carpio, for which fertilized eggs of C. carpio were exposed to different sublethal concentration of imidacloprid and 48 hrs LC50 was calculated as 78 ppm. Three different sublethal concentration of imidacloprid were selected for further experimentation (7.8 ppm, 15.6 ppm and 23.4 ppm) along with control. Hatchlings developed from treated and control eggs were fixed in Bouins fixative to analyze the histopathological effects of imidacloprid on brain, eyes and gills. The findings depicted several histopathological degenerations in brain, eyes and gills including neuronal degeneration, necrosis, vacuolation, Pyknotic nuclei, fragmented nuclei, mononuclear infiltration, spongiosis, reduced retinal pigmentation, ruptured lens tissue, uplifted sclerotic layer, ruptured arterial wall and degenerated lamellae.

Keywords

Brain, Cyprinus carpio, Eye, Gills, Histopathology, Imidacloprid.
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  • Effect of Imidacloprid on Histopathological Alterations of Brain, Gills and Eyes in hatchling carp (Cyprinus carpio L.)

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Authors

Harkrishan
Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana, India
Priyanka Saraf
Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana, India
Anil K. Tyor
Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana, India
Jitender K. Bhardwaj
Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana, India

Abstract


Imidacloprid, a neonicotinoid pesticide is used globally to control insect pest in rural and urban area where it enters into and contaminate the aquatic ecosystem. The present study was designed to study the toxic impacts of imidacloprid on developing hatchlings of Cyprinus carpio, for which fertilized eggs of C. carpio were exposed to different sublethal concentration of imidacloprid and 48 hrs LC50 was calculated as 78 ppm. Three different sublethal concentration of imidacloprid were selected for further experimentation (7.8 ppm, 15.6 ppm and 23.4 ppm) along with control. Hatchlings developed from treated and control eggs were fixed in Bouins fixative to analyze the histopathological effects of imidacloprid on brain, eyes and gills. The findings depicted several histopathological degenerations in brain, eyes and gills including neuronal degeneration, necrosis, vacuolation, Pyknotic nuclei, fragmented nuclei, mononuclear infiltration, spongiosis, reduced retinal pigmentation, ruptured lens tissue, uplifted sclerotic layer, ruptured arterial wall and degenerated lamellae.

Keywords


Brain, Cyprinus carpio, Eye, Gills, Histopathology, Imidacloprid.

References





DOI: https://doi.org/10.18311/ti%2F2020%2Fv27i1%262%2F25386