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Analysis of Genetic Diversity of Plumbago zeylanica L.


Affiliations
1 P.T.M.T.M College, Department of Botany, Kamuthi, Tamilnadu, India
2 St. Xavier’s College (Autonomous), Palayamkottai, Tamilnadu, India
 

Plumbago zeylanica is extensively used in herbal medicines and it has an anticancer compounds. The current investigation examines the genetic diversity of Plumbago zeylanica from five different populations of the Western Ghats using ISSR markers. Twenty primers were screened of which twelve were found to be the most informative. Based on Jaccard's coefficient, the P. zeylanica populations confirm a high level of genetic variability or heterozygosity. The PZ1 (Kallar -Ooty) accession has the highest percentage (70.59 %) of polymorphism. The overall mean value of genetic diversity (HT) in the group is 0.43 whereas the diversity within populations (Hs) is 0.30. The overall locus of Genetic differentiation (GST) between populations is 0.30. Inter population gene flow (Nm = 1.28) shows that the genetic exchange among populations is limited. Thus the ISSR marker is useful to study the genetic diversity of Plumbago populations.

Keywords

Genetic Diversity, Inter Simple Sequence Repeats (ISSR), Plumbago zeylanica L.
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  • Analysis of Genetic Diversity of Plumbago zeylanica L.

Abstract Views: 559  |  PDF Views: 292

Authors

K. Dharmar
P.T.M.T.M College, Department of Botany, Kamuthi, Tamilnadu, India
A. John De Britto
St. Xavier’s College (Autonomous), Palayamkottai, Tamilnadu, India

Abstract


Plumbago zeylanica is extensively used in herbal medicines and it has an anticancer compounds. The current investigation examines the genetic diversity of Plumbago zeylanica from five different populations of the Western Ghats using ISSR markers. Twenty primers were screened of which twelve were found to be the most informative. Based on Jaccard's coefficient, the P. zeylanica populations confirm a high level of genetic variability or heterozygosity. The PZ1 (Kallar -Ooty) accession has the highest percentage (70.59 %) of polymorphism. The overall mean value of genetic diversity (HT) in the group is 0.43 whereas the diversity within populations (Hs) is 0.30. The overall locus of Genetic differentiation (GST) between populations is 0.30. Inter population gene flow (Nm = 1.28) shows that the genetic exchange among populations is limited. Thus the ISSR marker is useful to study the genetic diversity of Plumbago populations.

Keywords


Genetic Diversity, Inter Simple Sequence Repeats (ISSR), Plumbago zeylanica L.

References





DOI: https://doi.org/10.15613/sijrs%2F2015%2Fv2i1%2F80081