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The present investigation was carried out to analyse the chemical nature of Indigofera trita using UV-VIS spectroscopy, Fourier Transform Infrared Spectroscopy, Nuclear Magnetic Resonance (1H and 13C NMR), High Performance Liquid Chromatography and Gas Chromatography Mass Spectroscopy. The results of FT-IR studies confirmed the presence of primary and secondary amine and amides, alcohol, phenols, carboxylic acid, alkanes, alkyne, oximes and aldehyde. GC-MS analysis provides 9 compounds of this four are therapeutically potent. The major identified compounds are Octadecanoic acid (43.67%), 8-Carbethoxy-1,4,5,6,7,8-hexahydropyrrolo[2,3-b]azepin-4-one-3-carboxylic acid (30.5%), 4H-1- Benzopyran-4-one,3,5,7-trihydroxy-2-phenyl (18.6%). HPLC chromatogram of the isolated amorphous compound showed the presence of alkaloid (Choline) with single peak in specific retention time (5.134). The results of the present study indicated I. trita contains medicinally important compounds which are traditionally used for various diseases.


GC-MS, HPLC, Indigofera trita, Leguminosae, Octadecanoic Acid, Phytoconstituents
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