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In Vitro Propagation of a Sporadic Medicinal Plant Cleome monophylla L


Affiliations
1 PG and Research, Department of Botany, Vellalar College for Women (Autonomous), Erode - 638 012, Tamil Nadu, India
 

A sporadic medicinal plant Cleome monophylla L. comes under the family Capparidaceae selected for the in vitro regeneration. The leaf explants showed differential responses to various combination and concentrations of BAP, TDZ and NAA and produced yellow to green friable and compact and dense calli. The maximum amount of callus per explants was produced on MS medium with 4.44 µM of BAP, 1.16 µM of TDZ and 1.135 µM of NAA combination and concentration gave the 84% response and produced green compact calli, 19.98 ± 0.03 shoots from the callus and the maximum mean number of ischolar_mainlets per culture was also noted (15 ± 0.57) in this combination with MS medium of half strength. The in vitro developed entire plantlets were fruitfully acclimatized to thermocol cups and earthen pots.

Keywords

Callogenesis, Capparidaceae, Cleome monophylla, Growth Regulators, Shoot and Root Induction
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  • Sharma K, Dubey S. Biotechnology and conservation of medicinal plants. J Exp Sci. 2011; 2(10):60–1.

  • Prakas S, Staden JV. Micropropagation of Hoslundia opposita Vahl - A valuable medicinal plant. South African J Botany. 2007; 73:60–3. https://doi.org/10.1016/j.sajb.2006.07.001

  • Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962; 15:473–97. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x.

  • Dietert JM. In vitro regeneration from excised leaf discs of three Brassica species. Plant Sci Lett. 1982; 26:233–40. https://doi.org/10.1016/0304-4211(82)90096-7.

  • Das R. Genotype specificity in in vitro callus induction of Brassica campestris cvs. B - 54, Th - 71 and B - 9. Exp Genet. 1991; 7(1/2):72–9.

  • Shah RU, Munir I. Efficient in vitro regeneration through somatic embryogenesis from callus induction method for Brassica carinata. Sarhad J Agri. 2019; 35(1):314–9. https://doi.org/10.17582/journal.sja/2019/35.1.314.319.

  • Sirangi SK. Induction of callus and shoot proliferation of a medicinally important herb. Cleome chelidonii L.F. Int J Curr Res Biosci and Plant Biol. 2015; 2(7):79–82.

  • Kalimuthu K, Prabakaran R, Preetha V. Direct and indirect micropropagation of Turnera ulmifolia (L.) a medicinal plant. World J Pharma and Pharmaceut Sci. 2014; 3(8):785– 93.

  • Seyyedyousefi SR, Kaviani B, Dehkaei NP. The effect of different concentrations of NAA and BAP on micropropagation of Alstroemeria. Europe J Exp Biol. 2013; 3(5):133–6.

  • Rathore NS, Rathore N, Shekhawat NS. In vitro propagation and micromorphological studies of Cleome gynandra: A C4 model plant closely related to Arabidopsis thaliana. Acta Physiol Plant. 2013; 35(9):2691–8. https://doi.org/10.1007/s11738-013-1301-2.

  • Albarello N, Simoes C, Rosas PFG, Catro TC, Gianfaldoni MG, Callado CH, Mansur E. In vitro propagation of Cleome spinosa (Capparaceae) using explants from nursery-grown seedlings and axenic plants. In vitro Cell Dev Biol Plant. 2006; 42:601–6. https://doi.org/10.1079/IVP2006828.

  • Anburaj J, Singh CR, Kuberan T, Sundaravadivelan C, Kumar P. Effects of plant growth regulators on callus induction from leaf explants of Cleome viscosa. Res J Pharmaceut Biol and Chem. 2011; 2(2):576–83.


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  • In Vitro Propagation of a Sporadic Medicinal Plant Cleome monophylla L

Abstract Views: 392  |  PDF Views: 233

Authors

Vijayashalini P.
PG and Research, Department of Botany, Vellalar College for Women (Autonomous), Erode - 638 012, Tamil Nadu, India
Abirami P.
PG and Research, Department of Botany, Vellalar College for Women (Autonomous), Erode - 638 012, Tamil Nadu, India
Jayanthi G.
PG and Research, Department of Botany, Vellalar College for Women (Autonomous), Erode - 638 012, Tamil Nadu, India

Abstract


A sporadic medicinal plant Cleome monophylla L. comes under the family Capparidaceae selected for the in vitro regeneration. The leaf explants showed differential responses to various combination and concentrations of BAP, TDZ and NAA and produced yellow to green friable and compact and dense calli. The maximum amount of callus per explants was produced on MS medium with 4.44 µM of BAP, 1.16 µM of TDZ and 1.135 µM of NAA combination and concentration gave the 84% response and produced green compact calli, 19.98 ± 0.03 shoots from the callus and the maximum mean number of ischolar_mainlets per culture was also noted (15 ± 0.57) in this combination with MS medium of half strength. The in vitro developed entire plantlets were fruitfully acclimatized to thermocol cups and earthen pots.

Keywords


Callogenesis, Capparidaceae, Cleome monophylla, Growth Regulators, Shoot and Root Induction

References





DOI: https://doi.org/10.15613/sijrs%2F2018%2Fv5i2%2F207621