Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

A Development of An Analytical Spectrophotometeric Method for determination of Taurine in Energy Drinks


Affiliations
1 Department of Analytical and Food Chemistry, Damascus University, Damascus, Syrian Arab Republic
     

   Subscribe/Renew Journal


A novel, simple, rapid and sensitive spectrophotometric method has been developed for assay of taurine in energy drinks which is an important amino acid in body human and it is added to energy drinks to improve the mental and muscle performance. The proposed method allows selective determination of taurine in energy drinks, which are a mixture of many components such as caffeine, ginseng, vitamins, guarana, carbohydrates, colorants, preservative agents, etc. Ninhydrin was used as derivatizing agent to form an irreversible complex with taurine. The reaction of taurine with ninhydrin was completed within 20 minutes at 100˚C and the colored complex has been measured using spectrophotomer at λmax=568 nm. The optimal conditions of this reaction have been studied. Extraction method has been developed using cation exchange resin to extract taurine and eliminate the interferences of other components in energy drinks. The validation of developed method was performed according to ICH [Q2(R1)] guidelines. A precision was estimated statistically using standard deviation (SD=0.25) and relative standard deviation (RSD%=0.64). The calibration curve was linear with a correlation coefficient (R2 = 0.997). The recoveries were between [98.45-99.33] %. Then fifty samples of consumed energy drinks in Syria have been analyzed using the developed method to determine their content of taurine.

Keywords

Spectrophotometer, Taurine, Ninhydrin, Validation, Energy Drinks.
Subscription Login to verify subscription
User
Notifications
Font Size


  • - Draganov G, Pencheva I and Todorova K. UV-spectrophotometry determination of taurine in energy drink mixtures. International Journal of Nutrition and Food Sciences. 3 (2); 2014: 123-126.

  • - McConn B. Determination of taurine in energy drinks by high performance liquid chromatography. Concordia College Journal of Analytical Chemistry. 47 (3); 2012: 47-52.

  • - Wang X, Chi D, SU G, LI L and Shao L. Determination of taurine in biological samples by high performance liquid chromatography using 4-fluoro7-nitrobenzofurazan as a derivatizing agent. Biomed Environ Sci. 24 (5); 2011: 537-542.

  • - Kothandam H et al. Taurine, “A Key Amino Acid in the Drug Discovery”: A review. Available from: URL: http://www.jbiopharm.com

  • - Ivanov K et al. Analytical methods for quality and quantity control of energy drinks and food supplements, containing amino acids. International Journal of Nutrition and Food Sciences. 4 (1); 2015: 9-3.

  • - Laidlaw SA, Grosvenor M and Kopple JD. The taurine content of common foodstuffs. Journal of Parenteral and Enteral Nutrition. 14; 1990: 183-188.

  • - Todorova K, Pencheva I. Analytical approaches and methods in quality control procedures of energy food drinks containing caffeine and taurine. International Journal of Nutrition and Food Sciences. 4 (1); 2015: 1-8.

  • - Champan R, Suleiman M and Earm Y. Taurine and the heart: An article. Available from: URL: https://doi.org/10.1093/cvr/27.3.358

  • - Friedman M. Applications of the ninhydrin reaction for analysis of amino acids, peptides, and proteins to agricultural and biomedical sciences. J Agric Food Chem. 52 (3); 2004: 385-406.

  • - Job p. Advanced physicochemical experiments. Analytical chemistry, Edinburgh. 1964.

  • - Guideline ICH. Validation of analytical procedures: text and methodology. Q2 (R1). 4; 2005.


Abstract Views: 156

PDF Views: 0




  • A Development of An Analytical Spectrophotometeric Method for determination of Taurine in Energy Drinks

Abstract Views: 156  |  PDF Views: 0

Authors

Riham Kais Saoud
Department of Analytical and Food Chemistry, Damascus University, Damascus, Syrian Arab Republic
Fida Am Ali
Department of Analytical and Food Chemistry, Damascus University, Damascus, Syrian Arab Republic
Zaid Al Assaf
Department of Analytical and Food Chemistry, Damascus University, Damascus, Syrian Arab Republic

Abstract


A novel, simple, rapid and sensitive spectrophotometric method has been developed for assay of taurine in energy drinks which is an important amino acid in body human and it is added to energy drinks to improve the mental and muscle performance. The proposed method allows selective determination of taurine in energy drinks, which are a mixture of many components such as caffeine, ginseng, vitamins, guarana, carbohydrates, colorants, preservative agents, etc. Ninhydrin was used as derivatizing agent to form an irreversible complex with taurine. The reaction of taurine with ninhydrin was completed within 20 minutes at 100˚C and the colored complex has been measured using spectrophotomer at λmax=568 nm. The optimal conditions of this reaction have been studied. Extraction method has been developed using cation exchange resin to extract taurine and eliminate the interferences of other components in energy drinks. The validation of developed method was performed according to ICH [Q2(R1)] guidelines. A precision was estimated statistically using standard deviation (SD=0.25) and relative standard deviation (RSD%=0.64). The calibration curve was linear with a correlation coefficient (R2 = 0.997). The recoveries were between [98.45-99.33] %. Then fifty samples of consumed energy drinks in Syria have been analyzed using the developed method to determine their content of taurine.

Keywords


Spectrophotometer, Taurine, Ninhydrin, Validation, Energy Drinks.

References