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Anti-alzheimer's Activity of Aqueous Extract of Leaves of Murraya koenigii in Aluminium Chloride Induced Neurotoxicity in Rats


Affiliations
1 Department of Pharmacology, Malla Reddy College of Pharmacy, Dhulapally, Maisamaguda, Secunderabad 500100, Medchal, Telangana, India
2 Department of Pharmacy, Annamalai University, Chidambaram, T.N., India
     

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Objective: The present study was aimed to evaluate the protective effect of Murraya koenigii on aluminium chloride (AlCl3) induced neurobehavioral and pathological changes in Alzheimeric rats. Methods: Wistar albino rats were selected in this study and were divided into 6 groups (6each). Group I animals received saline and were considered as control. Group II rats were administered with AlCl3 (40mg/kg b.w, oral) for 35 days. Group III rats were treated with standard Vitamin E (100mg/kg b.w, p.o) and AlCl3 (40mg/kg b.w, oral). Group IV, V,VI received aqueous extract of leaves of Murraya koenigii (100mg/kg b.w, p.o,200mg/kg b.w, p.o,400mg/kg b.w, p.o) and inducing agent AlCl3 (40mg/kg b.w, oral) .The rats were given respective treatment for 35 days and behavioural parameters were determined on the 35th day. On the 35th day the rats were sacrificed and anti-oxidant parameters, brain acetylcholinesterase content were determined. Results: Oral administration of aqueous extract of leaves of Murraya koenigii at doses 100,200, 400mg/kg body weight showed improvements in behavioural parameters when compared to AlCl3 induced rats; showed increase in superoxide dismutase, catalase, reduced glutathione and decreased levels of malondialdehyde and also a showed decrease in brain acetylcholinesterase content when compared to AlCl3 induced rats. Conclusion: This study strongly suggested that M koenigii could possibly restore the altered neurological capacities and antioxidant power in rats, and it could even be a good alternative to chemical medicines against AlCl3 induced neurotoxicity.

Keywords

Alzheimer’s Disease, Alcl3, Antioxidant parameters, Behavioural parameters, Murraya koenigii.
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  • Kaur K, Kaur K, Kaur M. Recent advances in Alzheimer's disease: causes and treatment. IJPPS 2015;8(2):8-15.

  • Yadav YC, Jain A, Deb L. A review: Neuropharmacological screening techniques for pharmaceuticals. IJPPS 2010;2 (2):10-14.

  • Braidy N, Poljak A, Marjo C, Rutlidge H, Rich A, Jayasena T, et al. "Metal and complementary molecular bioimaging in Alzheimer's disease." Front Aging Neuroscience 2014; 6(138):1-14.

  • Bukowska. "Toxicity of 2, 4-dichlorophenoxyacetic acid – molecular mechanisms." Polish Journal of Environmental Studies and Sciences 2006;15(3):365-74.

  • Cakmak G, Zorlu F, Severcan M, and Severcan F. "Screening of protective effect of amifostine on radiation-induced structural and functional variations in rat liver microsomal membranes by FT-IR spectroscopy." Analytical Chemistry 2011;7(83): 2438-44.

  • Kawahara M and Kato-Negishi M, "Link between aluminium and the pathogenesis of Alzheimer's disease: the integration of the aluminium and amyloid cascade hypotheses." International Journal of Alzheimer’s disease. 2011;1-17.

  • Shati A, Elsaid FG, and Hafez EE. "Biochemical and molecular aspects of aluminium chloride-induced neurotoxicity in mice and the protective role of Crocus sativus L. extraction and honey syrup." Neuroscience ,2011; 175:66-74.

  • Sethi P, Jyoti A, Singh R, Hussain E, and Sharma D. "Aluminium-induced electrophysiological, biochemical and cognitive modifications in the hippocampus of ageing rats." NeuroToxicology 2008; 29:1069–79.

  • Niu P. Y, Niu Q, Zhang Q. L, Wang L. P, Di-Gioacchino M, and Boscolo P. "Aluminium impairs rat neural cell mitochondria in vitro." International Journal of Immunopathology and Pharmacology 2005;18:683–9.

  • Esparza JL, Gómez M, Rosa Nogues M, Paternain JL, Mallol J, and Domingo JL. "Melatonin reduces oxidative stress and increases gene expression in the cerebral cortex and cerebellum of aluminium exposed rats." Journal of pineal research 2005; 39:129-136.

  • Moshtaghie A. "Aluminium administration on acetylcholinesterase activity of different regions of rat brain." Medical Journal of Islamic Academy Sciences 1999;12:105–8.

  • Pan R, Qiu S, Lu DX and Dong J. "Curcumin improves learning and memory ability and its neuroprotective mechanism in mice." Chinese Medical Journal (English Edition) 2008;121(9):832-39.

  • Prasad KD, Harsha SHS, Kumar DY, Neelima KSSN. Global Journal of Medical Research 2014;13.

  • Parmar S, Gangwal A, Sheth N. Mast cell membrane stabilization and anti-histaminic actions — possible mechanism of action of anti-inflammatory action of Murraya koenigii. Journal of Current Pharma Research 2010;2(1):21-5.

  • Yih Yih Kok S , Mooi LY , Ahmad K , Sukari MA, Mat N, Rahmani M et.al. AntiTumour Promoting Activity and Antioxidant Properties of Girinimbine Isolated from the Stem Bark of Murraya koenigii, Molecules 2012;17(4):4651-60.

  • Mani V, Ramasamy K, Ahmad A, Parle M, Shah SAA, Majeed AB. Protective effects of total alkaloidal extract from Murrayakoenigii leaves on experimentally induced dementia. Food and Chemical Toxicology 2012;50(3–4):1036–104

  • Kamila S, Madhav NVS, Sarkar CN. Safety and Efficacy Study of Herbal Polyphyto Formulations: for its learning and memory enhancing properties. International Journal of Pharmacy and Pharmaceutical Sciences 2015; 8(7):119-123.

  • Ellman GL, Courtney KD, Andres Jr V, Feather-stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology 1961;7:88-95.

  • Kumar V, Gill KD. Oxidative stress and mitochondrial dysfunction in aluminium neurotoxicity and its amelioration: a review. Neurotoxicology 2014;41:154-66.

  • Sharma DR, Wani WY, Sunkaria A, Kandimalla RJ, Verma D, Cameotra SS, et al. Quercetin protects against chronic aluminium induced oxidative stress and ensuing biochemical, cholinergic, and neurobehavioral impairments in rats. Neurotoxicity Research 2013;23(4):336-57.

  • Kumar S. Aluminium-induced changes in the rat brain serotonin system. Food and Chemical Toxicology 2002;40(12):1875-80.

  • Lakshmi BVS, Muvvala S, Aniska M. Neuroprotective role of hydroalcoholic extract of Vitis vinifera against aluminium-induced oxidative stress in rat brain. Neurotoxicology 2014;41:73-9.

  • Nachmansohn D. Chemical and molecular basis of nerve activity. New York: Academic Press 1975;120.

  • Poulin B, Butcher A, McWilliams P, Bourgognon JM, Pawlak R, Kong KC et al. The M3-muscarinic receptor regulates learning and memory in a receptor phosphorylation/ arrestin-dependent manner. Proceedings of National Academy of Sciences 2010;107(20): 9440-5.

  • Hu H, Yang Li XP, Chen GH. Effect of aluminium chloride on motor activity and species-typical behaviours in mice. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2005;23(2):132-5.

  • Baraldi T, Schowe NM, Balthazar J, Monteiro-Silva KC, Albuquerque MS, Buck HS et al. Cognitive stimulation during lifetime and in the aged phase improved spatial memory, and altered neuroplasticity and cholinergic markers of mice. Experimental Gerontology 2013;48(8):831-8.

  • Park D, Yang YH, Bae DK, Lee SH, Yang G, Kyung J, et al. Improvement of cognitive function and physical activity of ageing mice by human neural stem cells over-expressing choline acetyltransferase. Neurobiology of Aging 2013;34(11):2639-46.

  • Strunecka A, Grof P. The role of glucose in the pathogenesis of Alzheimer's disease revisited: what does it tell us about the therapeutic use of lithium Central Nervous System Agents Medicinal Chemistry 2006;6(2):175-92.

  • Wu ZH, Du YM, Xue H, Wu YS, Zhou B. Aluminum induces neurodegeneration and its toxicity arises from increased iron accumulation and reactive oxygen species (ROS) production. Neurobiological Aging 2012; 33(1):199.

  • Belaeid-Nouira Y, Bakhta H, Bouaziz M. Study of lipid profile and parieto-temporal lipid peroxidation in AlCl3 mediated neurotoxicity modulatory effect of fenugreek seeds. Lipids Health Disease 2012;11:16.

  • Kumar V, Gill KD. Oxidative stress and mitochondrial dysfunction in aluminium neurotoxicity and its amelioration: a review. Neurotoxicology 2014;41:154-66.

  • Nehru B, Anand P. Oxidative damage following chronic aluminium exposure in adult and pup rat brains. Journal of Trace Elements in Medicine and Biology 2005;19(2–3): 203-8.


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  • Anti-alzheimer's Activity of Aqueous Extract of Leaves of Murraya koenigii in Aluminium Chloride Induced Neurotoxicity in Rats

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Authors

B. Maheswari Reddy
Department of Pharmacology, Malla Reddy College of Pharmacy, Dhulapally, Maisamaguda, Secunderabad 500100, Medchal, Telangana, India
C. K. Dhanapal
Department of Pharmacy, Annamalai University, Chidambaram, T.N., India
B. V. S. Lakshmi
Department of Pharmacology, Malla Reddy College of Pharmacy, Dhulapally, Maisamaguda, Secunderabad 500100, Medchal, Telangana, India

Abstract


Objective: The present study was aimed to evaluate the protective effect of Murraya koenigii on aluminium chloride (AlCl3) induced neurobehavioral and pathological changes in Alzheimeric rats. Methods: Wistar albino rats were selected in this study and were divided into 6 groups (6each). Group I animals received saline and were considered as control. Group II rats were administered with AlCl3 (40mg/kg b.w, oral) for 35 days. Group III rats were treated with standard Vitamin E (100mg/kg b.w, p.o) and AlCl3 (40mg/kg b.w, oral). Group IV, V,VI received aqueous extract of leaves of Murraya koenigii (100mg/kg b.w, p.o,200mg/kg b.w, p.o,400mg/kg b.w, p.o) and inducing agent AlCl3 (40mg/kg b.w, oral) .The rats were given respective treatment for 35 days and behavioural parameters were determined on the 35th day. On the 35th day the rats were sacrificed and anti-oxidant parameters, brain acetylcholinesterase content were determined. Results: Oral administration of aqueous extract of leaves of Murraya koenigii at doses 100,200, 400mg/kg body weight showed improvements in behavioural parameters when compared to AlCl3 induced rats; showed increase in superoxide dismutase, catalase, reduced glutathione and decreased levels of malondialdehyde and also a showed decrease in brain acetylcholinesterase content when compared to AlCl3 induced rats. Conclusion: This study strongly suggested that M koenigii could possibly restore the altered neurological capacities and antioxidant power in rats, and it could even be a good alternative to chemical medicines against AlCl3 induced neurotoxicity.

Keywords


Alzheimer’s Disease, Alcl3, Antioxidant parameters, Behavioural parameters, Murraya koenigii.

References