Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Comparative Evaluation of Baicalein from Oroxylum indicum by using Conventional and Non-Conventional Extraction Methodology


Affiliations
1 Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad - 431001, Maharashtra, India
     

   Subscribe/Renew Journal


Baicalein (5, 6, 7-trihydroxyflavone) is flavonoid isolated from Oroxylum indicum belonging to family Bignoniaceae. Baicalein was extracted from O. indicum leaves by conventional and non-conventional extraction methodologies viz. Maceration, Soxhlet assisted (SAE), Ultra-sonic assisted (UAE) and accelerated solvent extraction (ASE). Ethanol was optimized among the various organic solvents used for the maceration process. All the extracts were compared with respect to the percentage yield obtained and the concentration of the baicalein in the different extracts. Among all the extraction methodologies Accelerated solvent extraction method proved the best in terms of percentage yield and concentration of baicalein. This method showed a maximum percent yield about 8.21% of the total extracts and high concentration of about 1.481 ng of baicalein in the extracts respectively, using pre-optimized ethanol as an extraction solvent. The accelerated solvent extraction method was found to be more efficient for the extraction of baicalein from leaves of O. indicum.

Keywords

Baicalein, Maceration, Soxhlet Assisted Extraction, Ultrasonic Assisted Extraction, Accelerated Solvent Extraction, HPLC.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Singh D. Sharma SK. Sharma P. Sharma R. A Comparative Study of Isolated Phytosteroles from Different Plant Parts of Selected Cassia Species. Asian Journal of Research in Chemistry. 2014: 7(2); 171-174.

  • Tatke P. Rajan M. Comparison of Conventional and Novel Extraction Techniques for the Extraction of Scopoletin from Convolvulus Pluricaulis. Pharmaceutical Research. 2013: 48(1); 27-31.

  • Gupta A, Naraniwal M, Kothari V. Modern extraction methods for the preparation of bioactive plant extracts. International Journal of Applied and Natural Sciences. 2012: 1(1); 8-26.

  • Azwanida NN. A Review on the Extraction Methods Uses in Medicinal Plants, Principle, Strength, and Limitation. Medicinal and Aromatic Plants. 2015: 4; 1-6.

  • Senthil MR. Khan I. Perumal P. Quantitative Analysis of Glycyrrhizic Acid in Crude Drug and its Herbal Formulation by HPLC. Asian Journal of Research in Chemistry. 2010: 3(3); 703- 706.

  • Wandhare MD. Deokate UA. Khadabadi SS, Hadke SP. Sawarkar HA. Comparative Estimation of Curcumin Content from Marketed Herbal Anti Rheumatic Tablets Formulation. Asian Journal of Research in Chemistry. 2009: 2(3); 340-343.

  • Ganesan B. Perumal P. Manickam VB. Srikakolapu SR. Gotteti SD. Thirumurthy LS. Simultaneous Determination of Strychnine and Brucine in Herbal Formulation by RP-HPLC. Asian Journal of Research in Chemistry. 2010: 3(3); 720-723.

  • Chaurasia A. Kharya MD. Evaluation and marker quantification of antidiabetic herbal tablets: Stevi Tab and Andro Tab by HPLC method. Research Journal of Pharmaceutical Dosage Forms and Technology. 2013: 5(1); 17-21.

  • Thangabalan B. Salomi M. Sunitha N. Manohar Babu S. Development of validated RP-HPLC method for the estimation of Itraconazole in pure and pharmaceutical dosage form. Asian Journal Pharmaceutical Analysis. 2013: 3(4); 119-123.

  • Chowdhury SR. Maleque M. Shihan MH. Development and Validation of a Simple RP-HPLC Method for Determination of Caffeine in Pharmaceutical Dosage Forms. Asian Journal Pharmaceutical Analysis. 2012: 2(1); 01-04.

  • Patel B. Dedania Z. Dedania R. Chetan Ramolia C. Sagar VG. Mehta RS. Simultaneous Estimation of Lansoprazole and Domperidone in CombinedDosage Form by RP-HPLC. Asian Journal of Research in Chemistry. 2009: 2(2); 210-212.

  • Singh M. Kashkhedikar SG. Soni L. Garg A. Gandhi T. Patel A. Development of RP-HPLC Method for Estimation of Carvedilol in Tablet Formulations. Research Journal of Pharmacy and Technology. 2008: 1(1); 18-22.

  • Dedania Z. Dedania R. Karkhanis V. Sagar VG. Sheth NR. RP-HPLC Method for Simultaneous Estimation of Omeprazole and Ondansetron in Combined Dosage Forms. Asian Journal of Research in Chemistry. 2009: 2(2); 108-111.

  • Preety A and Sharma S. A review on Oroxylum indicum (L.) Vent: an important medicinal tree. International Journal of Research in Biological Sciences. 2016: 6(1); 7-12.

  • Lawania R, Mishra A, Gupta R. Oroxylum indicum: A Review. Pharmacognosy Journal. 2010: 2(9); 304-310.

  • Upaganlawar A, Tenpe CR., Yeole PG. Anti-inflammatory activity of aqueous extract of Oroxylum indicum vent. Leaves extract- preliminary study. Pharmacologyonline. 2009: 1; 22-26.

  • Nakahara K, Onishi-Kameyama M, Ono H, Yoshida M, Trakoontivakorn G. Antimutagenic activity against trp-P-1 of the edible Thai plant, Oroxylum indicum vent. Bioscience, Biotechnology, and Biochemistry. 2001: 65(10); 2358-2360.

  • Raghu, Satheesh George AV, Renju Krishna V., Sindhu KK. Bioactive properties of phenolics present in Oroxylum indicum – A review. Journal of Pharmacognosy and Phytochemistry. 2013: 2 (3); 23-27.

  • Zaveri M, Gohil P, Sunita Jain. Immunostimulant Activity of n-Butanol Fraction of Root Bark of Oroxylum indicum, vent. Journal of Immunotoxicology. 2006: 3; 83–99.

  • Tenpe CR. Upaganlawar A, Burle S, Yeole PG. In vitro antioxidant and preliminary hepatoprotective activity of Oroxylum indicum vent leaf extracts. Pharmacologyonline. 2009: 1; 35-43.

  • Satya Eswari J, Dhagat S, Naik S, Dibya S. Oroxylum indicum leaf extracts for screening of antimicrobial properties and phytochemicals. Pharm. Bioprocess. 2018: 6(1); 7–14.

  • Mao. AA. Oroxylum indicum Vent. - A potential anticancer medicinal plant. Indian journal of traditional knowledge. 2002: 1(1); 17-21.

  • Gupta R, Sharma V, Sharma N, Kumar N, Singh B. In vitro Antioxidant Activity from Leaves of Oroxylum indicum (L.) Vent. -A North Indian Highly Threatened and Vulnerable Medicinal Plant. Journal of Pharmacy Research. 2008: 1(1); 65-72.

  • Mishra SL. Sinhamahapatra PK. Nayak A, Das R, Sannigrahi S. In vitro Antioxidant Potential of Different Parts of Oroxylum indicum: A Comparative Study. Indian Journal of Pharmaceutical Sciences. 2010: 72 (2); 267-269.

  • Khandhar M, Shah M, Santani D, Jain S. Antiulcer Activity of the Root Bark of Oroxylum indicum. Against Experimental Gastric Ulcers. Pharmaceutical Biology. 2008: 44(5); 363–370.

  • Bie B, Sun J, Guo Y, Li J, Jiang W, Yang J, Huang C, Li Z. Baicalein: A review of its anti-cancer effects and mechanisms in Hepatocellular Carcinoma. Biomed Pharmacother. 2017: 93; 1285-1291.

  • Hari Babu T, Manjulatha K, Suresh Kumar G, Hymavathi A, Tiwari A, Purohit M, Madhusudana Rao J, Suresh Babu K. Gastroprotective flavonoid constituents from Oroxylum indicum Vent. Bioorganic and Medicinal Chemistry Letters. 2010: 20; 117–120.

  • Donald G, Hertzer K, Guido E. Baicalein – An Intriguing Therapeutic Phytochemical in Pancreatic Cancer. Curr Drug Targets. 2012: 13(14); 1772–1776.

  • Zandi K, Boon-Teong T, Sing-Sin S, Pooi-Fong W, Mustafa M. S, Bakar S. A. Novel antiviral activity of baicalein against dengue virus. BMC Complementary and Alternative Medicine. 2012:12(214); 1-9.

  • Zaveria M, Khandhar A, Jain S. Quantification of Baicalein, Chrysin, Biochanin-A and Ellagic Acid in Root Bark of Oroxylum indicum by RP-HPLC with UV Detection. Eurasian Journal of Analytical Chemistry. 2008: 3(2); 245-257.

  • Liu R, Xu L, Li A, Sun A. Preparative isolation of flavonoid compounds from Oroxylum indicum by high-speed counter-current chromatography by using ionic liquids as the modifier of two-phase solvent system. Journal of Separation science. 2010: 33; 1058–1063.

  • Yuan Y, Hou W, Tang M, Luo H, Li-Juan C, Hugh Guan Y, Ian A. Separation of Flavonoids from the Leaves of Oroxylum indicum by HSCCC. Chromatographia. 2008: 68(11-12); 885-892.

  • Roy M, Nakahara K, Thalang V, Trakoontivakorn G, Takenaka M, Isobe S, Tsushida T. Baicalein, a flavonoid extracted from a methanolic extract of Oroxylum indicum inhibits proliferation of a cancer cell line in vitro via induction of apoptosis. Pharmazie. 2007: 62(2); 49-153.


Abstract Views: 167

PDF Views: 0




  • Comparative Evaluation of Baicalein from Oroxylum indicum by using Conventional and Non-Conventional Extraction Methodology

Abstract Views: 167  |  PDF Views: 0

Authors

Sachin Bhusari
Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad - 431001, Maharashtra, India
Supriya Morey
Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad - 431001, Maharashtra, India
Kanchan Nikam
Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad - 431001, Maharashtra, India
Pravin Wakte
Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad - 431001, Maharashtra, India

Abstract


Baicalein (5, 6, 7-trihydroxyflavone) is flavonoid isolated from Oroxylum indicum belonging to family Bignoniaceae. Baicalein was extracted from O. indicum leaves by conventional and non-conventional extraction methodologies viz. Maceration, Soxhlet assisted (SAE), Ultra-sonic assisted (UAE) and accelerated solvent extraction (ASE). Ethanol was optimized among the various organic solvents used for the maceration process. All the extracts were compared with respect to the percentage yield obtained and the concentration of the baicalein in the different extracts. Among all the extraction methodologies Accelerated solvent extraction method proved the best in terms of percentage yield and concentration of baicalein. This method showed a maximum percent yield about 8.21% of the total extracts and high concentration of about 1.481 ng of baicalein in the extracts respectively, using pre-optimized ethanol as an extraction solvent. The accelerated solvent extraction method was found to be more efficient for the extraction of baicalein from leaves of O. indicum.

Keywords


Baicalein, Maceration, Soxhlet Assisted Extraction, Ultrasonic Assisted Extraction, Accelerated Solvent Extraction, HPLC.

References