Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Visible Extractive Spectrophotometric Estimation of Sofosbuvir in Bulk and in Pharmaceutical Formulations


Affiliations
1 Department of Chemistry, Sri Krishnadevaraya University, Anantapur-515003, India
2 Analytical Department, Vishnu Chemicals Limited, Jeedimetla, Hyderabad, India
3 Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy Mount Pleasant, Hyderabad-500 034., India
     

   Subscribe/Renew Journal


Two simple, accurate, rapid and sensitive methods (A and B) have been developed for the estimation of Sofosbuvir in pharmaceutical dosage form. The Method A is based on reaction of Sofosbuvir with ferric chloride and 1,10-phenanthroline to form a blood red colored chromogen. The Method B is based on reaction of Sofosbuvir with ferric chloride and 2,2'- bipyridyl to form a red colored ferroin complex measured at 520 nm, against reagent blank. These Methods exhibit maximum absorption at 510 nm and 520 nm respectively and obey the Beer’s law in the concentration range of 10-140 μg/mL and. 5-50 μg /mL respectively. The Methods have been statistically evaluated and were found to be precise and accurate. The proposed methods are economical and sensitive for the estimation of Sofosbuvir in bulk drug and in its formulations.

Keywords

UV-Visible Spectrophotometry, Sofosbuvir, Ferric Chloride, 1, 10-Phenanthroline, and 2, 2’ Bipyridyl.
Subscription Login to verify subscription
User
Notifications
Font Size


  • The American Society of Health-System Pharmacists. Archived from the original on 2016-12-01. Retrieved Nov 30, 2016.

  • Divya Rajagopal for the Economic Times. Sept 12, 2015. Can Indian generic makers find gold with a blockbuster Hepatitis C drug?

  • 3."Sofosbuvir (Sovaldi)-Treatment-Hepatitis C Online". www.hepatitisc.uw.edu. Archived from the original on 23 December 2016. Retrieved 8 January 2017.

  • FDA, News Release (June 28, 2016). "FDA approves Epclusa for treatment of chronic Hepatitis C virus infection". Archived from the original on June 3, 2017.

  • "Sovaldi 400 mg film coated tablets - Summary of Product Characteristics (SPC) - (eMC)". www.medicines.org.uk. Archived from the original on 31 January 2017. Retrieved 8 January 2017.


Abstract Views: 188

PDF Views: 0




  • Visible Extractive Spectrophotometric Estimation of Sofosbuvir in Bulk and in Pharmaceutical Formulations

Abstract Views: 188  |  PDF Views: 0

Authors

Nakka Raju
Department of Chemistry, Sri Krishnadevaraya University, Anantapur-515003, India
P. Raveendra Reddy
Department of Chemistry, Sri Krishnadevaraya University, Anantapur-515003, India
P. Janaki Pathi
Analytical Department, Vishnu Chemicals Limited, Jeedimetla, Hyderabad, India
N. Appala Raju
Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy Mount Pleasant, Hyderabad-500 034., India

Abstract


Two simple, accurate, rapid and sensitive methods (A and B) have been developed for the estimation of Sofosbuvir in pharmaceutical dosage form. The Method A is based on reaction of Sofosbuvir with ferric chloride and 1,10-phenanthroline to form a blood red colored chromogen. The Method B is based on reaction of Sofosbuvir with ferric chloride and 2,2'- bipyridyl to form a red colored ferroin complex measured at 520 nm, against reagent blank. These Methods exhibit maximum absorption at 510 nm and 520 nm respectively and obey the Beer’s law in the concentration range of 10-140 μg/mL and. 5-50 μg /mL respectively. The Methods have been statistically evaluated and were found to be precise and accurate. The proposed methods are economical and sensitive for the estimation of Sofosbuvir in bulk drug and in its formulations.

Keywords


UV-Visible Spectrophotometry, Sofosbuvir, Ferric Chloride, 1, 10-Phenanthroline, and 2, 2’ Bipyridyl.

References