Research Journal of Pharmacy and Technology

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Multiple Shoot Regeneration in Seed-derived Immature Leaflet Explants of Red Dragon Fruit (Hylocereus costaricensis)


Affiliations
1 Department of Biotechnology, KLEF, Green Fields, Vaddeswaram, Guntur, India
2 Department of Biotechnology, Gandhi Institute of Technology and Management, Visakhapatnam, Andhra Pradesh, India
3 Prof. Jaya Shankar Telangana State Agricultural University, Hyderabad, Telangana, India
4 Rasi Seeds Pvt. Ltd. Tamilnadu, India
     

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Dragon fruit which belongs to Cacteceae family was once widely grown as ornamental plant and is now considered as fruit crop. Though there were many reports on propagating dragon fruit inadequate information is available on obtaining best quality pant material. Hence the present work is carried out to find the effect of phyto harmones, 6-Benzylaminopurine (BAP) and Naphthalene acetic acid (NAA) on callus formation, shoot and ischolar_main regenerations from dragon fruit in vitro using stem cuttings as explants. Stem explants in Murashige Skoog (MS) medium containing 3mg/l BAP and 0.5mg/l NAA exhibited highest in vitro response for callus formation. Further, maximum number of shoots (13.2) was regenerated from explants when MS basal medium was supplemented with 3mg/l BAP and 0.5mg/l NAA. Within four weeks somatic shoots were raised from explants. It took 70 days for shoots to grow 2.0cm long. Subsequently regenerated shoots were transferred to ischolar_maining medium supplemented with 3mg/l BAP and 0.5mg/l NAA which proved to be effective concentrations for the growth and development of ischolar_mains (4.8) in vitro.

Keywords

Callus, Dragon Fruit, MS Basal Medium, NAA, BAP, Somatic Shoots.
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  • Multiple Shoot Regeneration in Seed-derived Immature Leaflet Explants of Red Dragon Fruit (Hylocereus costaricensis)

Abstract Views: 203  |  PDF Views: 0

Authors

D. Pedda Kasim
Department of Biotechnology, KLEF, Green Fields, Vaddeswaram, Guntur, India
N. Sai Kishore
Department of Biotechnology, Gandhi Institute of Technology and Management, Visakhapatnam, Andhra Pradesh, India
P. Suneetha
Prof. Jaya Shankar Telangana State Agricultural University, Hyderabad, Telangana, India
K. Bramareswara Rao
Rasi Seeds Pvt. Ltd. Tamilnadu, India
M. Naresh Kumar
Department of Biotechnology, KLEF, Green Fields, Vaddeswaram, Guntur, India
M. S. R. Krishna
Department of Biotechnology, KLEF, Green Fields, Vaddeswaram, Guntur, India

Abstract


Dragon fruit which belongs to Cacteceae family was once widely grown as ornamental plant and is now considered as fruit crop. Though there were many reports on propagating dragon fruit inadequate information is available on obtaining best quality pant material. Hence the present work is carried out to find the effect of phyto harmones, 6-Benzylaminopurine (BAP) and Naphthalene acetic acid (NAA) on callus formation, shoot and ischolar_main regenerations from dragon fruit in vitro using stem cuttings as explants. Stem explants in Murashige Skoog (MS) medium containing 3mg/l BAP and 0.5mg/l NAA exhibited highest in vitro response for callus formation. Further, maximum number of shoots (13.2) was regenerated from explants when MS basal medium was supplemented with 3mg/l BAP and 0.5mg/l NAA. Within four weeks somatic shoots were raised from explants. It took 70 days for shoots to grow 2.0cm long. Subsequently regenerated shoots were transferred to ischolar_maining medium supplemented with 3mg/l BAP and 0.5mg/l NAA which proved to be effective concentrations for the growth and development of ischolar_mains (4.8) in vitro.

Keywords


Callus, Dragon Fruit, MS Basal Medium, NAA, BAP, Somatic Shoots.

References