Research Journal of Pharmacy and Technology

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A Comparative Evaluation Study of Citrus limetta and Metformin against Hyperlipidemia in Diabetic and Non-diabetic Rats


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1 Integral University, Lucknow-226026, (U.P), India
     

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Citrus limetta risso (Rutaceae) has been frequently used in Indian traditional medicine for the treatment of hyperlipidemia and promotion of weight loss. Studies also indicated towards the antihyperlipidemic potential of Metformin. This study was performed on high fat diet (HFD) induced hyperlipidemia in murine models in both presence and absence of diabetes which was induced by dose of 30mg/kg. Hyperlipidemia was induced by feeding rats with HFD for 28 days. Antihyperlipidemic effect of Citrus limetta peel extract (400mg/kg) and its main terpene component D-limonene (400mg/kg) was evaluated and compared with the effect of Metformin (200mg/kg) by the measurement of body weight gain, body mass index(BMI), food efficiency ratio, fasting blood glucose, Lee’s index, heart to body weight ratio, serum lipid profiles (triglycerides, total cholesterol, HDL-Cholesterol, LDL-Cholesterol, VLDL-Cholesterol), end organ weights (Liver, heart, kidneys and visceral fat pad) and antioxidant enzymes such as Thiobarbituric acid (TBARS), Superoxide dismutase (SOD), Catalase (CAT) and Glutathione (GSH). Histomorphological studies were also carried out. Citrus limetta extract, D-limonene and Metformin significantly reduced the weight gain percentage, total cholesterol, LDL and VLDL and increased the level of HDL-cholesterol and antioxidant enzyme level in liver tissue, also controlled the other parameters’ value within their normal ranges. Histomorphological study of fat tissue shows the normalization of swollen and slight deteriorated cells in the treated groups as compared to control groups. This study concludes that Citrus limetta extract and D-limonene have the antihyperlipidemic activity.

Keywords

Hyperlipidemia, Consumption, Accumulation.
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  • Lu Yanet al., Citrange fruit extracts Alleviate obesity associated metabolic disorder in high fat diet induced obese C57BL/6 mouse. International Journal of Molecule Science. 2013; 14:23736-237500; doi:10.3390/ijms 141223736.

  • N. Vidula Sri, Relationship of waist hip ratio and body mass index to the blood pressure of individuals in Chennai population. Research Journal of Pharmacy and Technology.2015. 8(7): 906-908.

  • Mopuri, et al. Evaluation of Antilipidemic and Anti Obesity Efficacy of Bauhinia purpurea Bark Extract on Rats Fed with High Fat Diet. Academic Journal of Plant Sciences.2010; 3(3): 104-107.

  • Sarvesh et al. Antihyperlipidemic activity of Achyranthes aspera Linn leaves on cholesterol induced hyperlipidemia in rats. Research Journal of Pharmacy and Technology. 2017; 10(1): 200-204.

  • Rashmi and Gowri, A study on obesity among children. Research Journal of Pharmacy and Technology. 2015; 8(8):1177-1178.

  • Karthika et al., Antioxidant potential of aqueous extract of Aeglemarmelos leaves. Research Journal of Pharmacy and Technology. 2016. 9(4): 391-393.

  • Charradi et al., High fat diet induced on oxidative stress in white adipose tissue and disturbed plasma transition metal in rats: Prevention by grape seed and skin extract. Journal of Physiological science. 2013; 63:445-55.

  • M. Vaishali. Antioxidants in health and disease. Research Journal of Pharmacy and Technology.2014; 7(4): 489-493.

  • Fatemah et al., The relationship between metabolic factors and quality of life aspects in type2 diabetes patients. Research Journal of Pharmacy and Technology.2017; 10(5): 1491-1496.

  • Roger Walker, Dyslipidemia, in clinical pharmacy and therapeutics, edited by Roger Walker & Clive Edwards (Churchill Livingstone, Spain) 2003:353.

  • Abijeth and Jothi Priya. Risk factors involved in coronary heart disease in relation with oral hygiene. Research Journal of Pharmacy and Technology. 2015; 8(8):997-1000.

  • Keshet al. High fat diet induced oxidative stress and its impact on metabolic syndrome: A review. Asian Journal of Pharmacology and Clinical Research. 2016; 9(1): 47-52.

  • Sun J. D-Limonene: Safety and Clinical Application. Alternative Medicine Revised. 2007, 12(3), 259-264.

  • Igimi et al. The use of d-limonene preparation as a dissolving agent of gallstones. American journal of digestive diseases.1976; 21: 926-939.

  • Javed et al., Chemical constituents, antimicrobial and antioxidant activity of essential oil of Citrus limettavar. Mitha (sweet lime) peel in Pakistan. African. Journal of Microbiology Research. 2013, 7(24), 3071-3077.

  • Akiyama et al., Dietary hesperidin exerts hypoglycemic and hypolipidemic effects in streptozotocin-induced marginal type 1 diabetic rats. Journal of Clinical Biochemistry and Nutrition. 2010, 46 (1), 87–92.

  • Ali et al., The influence of naringin on the oxidative state of rats with streptozotocin-induced acute hyperglycaemia, Zeitschrift fur Naturforschung. Section C. 2004; 59(9-10): 726–733.

  • Perez Y et al., Citrus limetta leaves extract antagonizes the hypertensive effect of angiotensin II. Journal of ethnopharmacology. 2010; 128(3):6114.

  • Kumar et al. Larvicidal efficacy of the Citrus limetta peel extracts against Indian strains of Anopheles stephensi Liston and Aedesaegypti L. Parasitology Research. 2012; 111(1):173-8.

  • Khan et al., Phytochemical and Pharmacological properties of Citrus limetta (Mosambi). Journal of Chemical and Pharmaceutical Research. 2016; 8(3): 555-563.

  • Tripathi, K. Essentials of medical pharmacology (7th ed.). New Delhi: Jaypee Brothers.2013.

  • Vijaya, et al. Lipid lowering activity of ethanolic extract of leaves of Aeglemarmelos (Linn) in hyperlipidaemic models of Wistar Albino rats, Indian Journal of Experimental Biology. 2009; 47:182-18.

  • Amey Holmes et al., Rat Models of Diet-Induced Obesity and High Fat/Low Dose Streptozotocin Type 2 Diabetes: Effect of Reversal of High Fat Diet Compared to Treatment with Enalapril or Menhaden Oil on Glucose Utilization and Neuropathic Endpoints. Journal of Diabetes research. 2015:307285-307285.doi.org/10.1155/2015/307285.

  • Konda VR et al., KDT501, a derivative form hops, normalizes glucose metabolism and body weight in rodent models of diabetes. PLoS One. 2014; 9(1):e87848.

  • Kundusen et al. Evaluation of Antihyperglycemic Activity of Citrus limetta Fruit Peel in Streptozotocin-Induced Diabetic Rats. ISRN Endocrinology. 2011; (2011): 869273.

  • Altunkaynak BZ, Ozbek E. Overweight and structural alterations of the liver in female rats fed a high-fat diet; A stereological and histological study. Turkish journal of gastroenterology. 2009; 20(2): 93-103.

  • Shamim et al Effect of Tinospora cordifolia (Guduchi) ischolar_main extract on cardiotoxicity in streptozocin induced diabetic rats. Asian Journal of Biomedical and Pharmaceutical sciences. 2015; 45: 12-19.

  • Sedlak J, Lindsay RH. Estimation of total, protein-bound and nonprotein sulfhydryl groups in tissues with Ellman's reagent, Analytical Biochemistry. 1968; 25:192-205.

  • Shanmukha et al., Antioxidant and nephroprotective activity of Spathodea campanulata bark against gentamicin induced nephrotoxicity. Pharmacology Online.2010;1: 666-675.

  • Kakkar et al., A modified spectrophotometric assay of superoxide dismutase. Indian Journal of Biochemistry and Biophysics.1984; 21:130-132.

  • Khatun et al., Antioxidant activity of the medicinal plant Coleus forskohliiBriq.African Journal of Biotechnology.2011; 10(13): 2530-2535.

  • Ohkawa et al., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry.1979; 95: 351-358.

  • Cohen et al., Measurement of catalase activity in tissues extracts. Analytical Biochemistry.1970; 34: 30-38.

  • Shokrzadeh et al., Effect of the aqueous green leaf extract of green tea (Camellia sinensis) on glucose level of rat. Pakistan Journal of Biology Science. 2006; 9(14): 2708-2711.

  • Buettner et al., High-fat diets: modeling the metabolic disorders of human obesity in rodents. Obesity (Silver Spring). 2007; 15(4):798-808.

  • Shetty and Karunakar. Antiobesity activity of ethanolic extract of Citrus maxima leaves on cafeteria diet induced and drug induced obese rats. Research Journal of Pharmacy and Technology.2016; 9(7): 907-912.

  • Srikanth and Murlidharan. Antihyperlipidemic activity of Sapindusemarginatusin triton WR-1339 induced albino rats. Research Journal of Pharmacy and Technology. 2009; 2(2): 319-323.

  • Manthey A, Grohmann K. Concentrations of hesperidin and other orange peel flavonoids on citrus processing byproducts. Journal of Agricultural and Food Chemistry. 1996; 44: 811-814.

  • Murdock DI, Allen WE. Germicidal effect of orange peel oil and D-limonene in water and orange juice. Food Technology. 1960; 14(9):441-5.

  • Berger RG, editor. Flavours and fragrances: chemistry, bioprocessing and sustainability. Springer Science & Business Media; 2007 Mar 6.

  • Matsuo et al. Body fat accumulation is greater in rats fed a beef tallow diet than in rats fed a safflower or soybean oil diet. Asia Pacific Journal of Clinical Nutrition.2002;11(4):302-8.

  • Weindruch R, Sohal RS. Caloric intake and aging. New England Journal of Medicine. 1997;337(14):986-94.

  • Ikewuchi et al., Weight reducing and hypocholeramic effects of aqueous leaf extract of Sansevierasenegambicaon sub chronic salt loaded rats: Implication for the reduction of cardiovascular risk. Research Journal of Pharmacy and Technology.2011; 4(5): 725-729.

  • Chang, S, et al. Metabolic differences between obesity-prone and obesity-resistant rats. American Journal of Physiology.1990; 259: R1103–R1110.

  • Rupesh and Sharma. Effect of Panica granatum Linn. (peel) on blood glucose level in normal and alloxan induced diabetic rats. Research journal of Pharmacy and Technology. 2012; 5(2): 226-227.

  • Lavie et al., To B or not to B: is non-high-density lipoprotein cholesterol an adequate surrogate for apolipoprotein B?.Mayo Clinic proceedings. 2010; 85(5): 446-50.

  • Jayakumar et al., Effect of ginger (Zingifer officinale) on lipids in rats fed atherogenic diet. Journal of Clinical Biochemistry and Nutrition. 1991; 27(5): 79–82.

  • Sowmya and Ananthi. Hypolipidemic activity of Mimosa pudica Linn on butter induced hyperlipidemia in rats. Asian journal of research in pharmaceutical sciences. 2011; 1(4): 123-126.

  • Asayama et al., Effect of obesity and troglitazone on expression of two glutathione peroxidases: Cellular and extracellular types in serum, kidney and adipose tissue. Free radical research. 2001; 34 (4): 337-47.

  • Slim et al., Susceptibility to hepatic oxidative stress in rabbits fed different animal and plant fats. Journal of American college of nutrition. 1996, 15 (3): 289-94.

  • Padamja et al., Therapeutic efficiency of Leucasaspera (Laminaceae) on lead acetate induced nephrotoxicity and oxidative stress in male wistar rats. Asian journal of research in chemistry. 2011. 4(10): 1515-1519.


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  • A Comparative Evaluation Study of Citrus limetta and Metformin against Hyperlipidemia in Diabetic and Non-diabetic Rats

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Authors

Ahmed Abdullah Khan
Integral University, Lucknow-226026, (U.P), India
Hefazat Hussain Siddiqui
Integral University, Lucknow-226026, (U.P), India
Tarique Mahmood Ansari
Integral University, Lucknow-226026, (U.P), India
Farogh Ahsan
Integral University, Lucknow-226026, (U.P), India

Abstract


Citrus limetta risso (Rutaceae) has been frequently used in Indian traditional medicine for the treatment of hyperlipidemia and promotion of weight loss. Studies also indicated towards the antihyperlipidemic potential of Metformin. This study was performed on high fat diet (HFD) induced hyperlipidemia in murine models in both presence and absence of diabetes which was induced by dose of 30mg/kg. Hyperlipidemia was induced by feeding rats with HFD for 28 days. Antihyperlipidemic effect of Citrus limetta peel extract (400mg/kg) and its main terpene component D-limonene (400mg/kg) was evaluated and compared with the effect of Metformin (200mg/kg) by the measurement of body weight gain, body mass index(BMI), food efficiency ratio, fasting blood glucose, Lee’s index, heart to body weight ratio, serum lipid profiles (triglycerides, total cholesterol, HDL-Cholesterol, LDL-Cholesterol, VLDL-Cholesterol), end organ weights (Liver, heart, kidneys and visceral fat pad) and antioxidant enzymes such as Thiobarbituric acid (TBARS), Superoxide dismutase (SOD), Catalase (CAT) and Glutathione (GSH). Histomorphological studies were also carried out. Citrus limetta extract, D-limonene and Metformin significantly reduced the weight gain percentage, total cholesterol, LDL and VLDL and increased the level of HDL-cholesterol and antioxidant enzyme level in liver tissue, also controlled the other parameters’ value within their normal ranges. Histomorphological study of fat tissue shows the normalization of swollen and slight deteriorated cells in the treated groups as compared to control groups. This study concludes that Citrus limetta extract and D-limonene have the antihyperlipidemic activity.

Keywords


Hyperlipidemia, Consumption, Accumulation.

References