Development and Validation of HPTLC Method for Simultaneous Estimation of Piperine and Scopoletin in Ajmodadi churna

In this recent study, several trials were made to develop a HPTLC method for quantification of scopoletin and piperine in the Ajmodadi formulation. HPTLC was done on pre-coated silica gel 60 F254 plates with a mobile phase of toluene:ethyl acetate:methanol:formic acid (3.9:3.9:0.3:1.7 v/v/v/v). The retardation factors for scopoletin 0.75 and for piperine was 0.86 and found a good and defined resolution peak. Densitometer analysis of scopoletin and piperine was carried out at 335 wavelength (nm). The developed method was validated as reported in ICH guidelines (Q2R1). A linearity study shows that scopoletin and piperine was linear, and the recovery studies revealed a recovery in between 98-102 % as per guideline. This method was established to be rapid, delicate, accurate and specific, thus, these methods may be used in the quantification of the drug in any polyherbal formulation.