Journal of Natural Remedies

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Bioactivity Screening and Identification of Secondary Metabolites from Fungal Endophytes of Carica papaya L. Leaves


Affiliations
1 Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka – 1205, Bangladesh
2 Dr. Mahbubur Rahman Mollah College, Matuail, Jatrabari, Dhaka – 1362, Bangladesh
3 Biological Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka – 1205, Bangladesh
4 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka – 1000, Bangladesh
 

Objectives: This work describes the chemical and in-vitro bioactivity studies on endophytic fungi derived from the leaves of Carica papaya L. Methods: Column Chromatography was employed for isolation of fungal metabolites. Nuclear Magnetic Resonance (NMR) spectral data was utilized to elucidate the structure of the isolated compounds and physicochemical properties of them were also examined. As a part of in-vitro bioactivity screening disc diffusion method, 1, 1-diphenyl-2- picryl-hydrazyl (DPPH) scavenging assay and brine shrimp lethality bioassay were conducted to evaluate antimicrobial, antioxidant and cytotoxic activities, respectively. Results: A total of four fungal isolates were identified as endophytic fungi and purified from the young leaves of Carica papaya. These fungi, encoded as CPLE-1, CPLE-2, CPLE-3 and CPLE-4, were identified up to the genus level on the basis of their macroscopic and microscopic characteristics as Colletotrichum sp., Carvularia sp., and next two as Alternaria sp., respectively. Chemical profiling and preliminary bioactivity screening of the fungal endophytes signified strain CPLE-3 with highest potentiality to produce bioactive compounds which directs its large scale cultivation. Ergosterol and Alternariol monomethyl ether were isolated from the ethyl acetate extract of the fungus, CPLE-3. Conclusion: Bioactivity and chemical screening of the endophytic fungal extracts of Carica papaya leaf along with the isolation of fungal secondary metabolites suggested these endophytic fungi to be possible source of bioactive leads for developing new and improved medicines.

Keywords

Alternaria, Alternariol Monomethyl Ether, Carvularia, Colletotrichum, Ergosterol
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  • Bioactivity Screening and Identification of Secondary Metabolites from Fungal Endophytes of Carica papaya L. Leaves

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Authors

Hossain Sohrab
Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka – 1205, Bangladesh
Israt Farha Lini
Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka – 1205, Bangladesh
Rabita Zinnurine
Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka – 1205, Bangladesh
Habibur Rahman
Dr. Mahbubur Rahman Mollah College, Matuail, Jatrabari, Dhaka – 1362, Bangladesh
Nadira Begum
Biological Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka – 1205, Bangladesh
Farhana Afroz
Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka – 1205, Bangladesh
Satyajit Roy Rony
Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka – 1205, Bangladesh
Suriya Sharmin
Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Dr. Qudrat-I-Khuda Road, Dhanmondi, Dhaka – 1205, Bangladesh
Choudhury Mahmood Hasan
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka – 1000, Bangladesh

Abstract


Objectives: This work describes the chemical and in-vitro bioactivity studies on endophytic fungi derived from the leaves of Carica papaya L. Methods: Column Chromatography was employed for isolation of fungal metabolites. Nuclear Magnetic Resonance (NMR) spectral data was utilized to elucidate the structure of the isolated compounds and physicochemical properties of them were also examined. As a part of in-vitro bioactivity screening disc diffusion method, 1, 1-diphenyl-2- picryl-hydrazyl (DPPH) scavenging assay and brine shrimp lethality bioassay were conducted to evaluate antimicrobial, antioxidant and cytotoxic activities, respectively. Results: A total of four fungal isolates were identified as endophytic fungi and purified from the young leaves of Carica papaya. These fungi, encoded as CPLE-1, CPLE-2, CPLE-3 and CPLE-4, were identified up to the genus level on the basis of their macroscopic and microscopic characteristics as Colletotrichum sp., Carvularia sp., and next two as Alternaria sp., respectively. Chemical profiling and preliminary bioactivity screening of the fungal endophytes signified strain CPLE-3 with highest potentiality to produce bioactive compounds which directs its large scale cultivation. Ergosterol and Alternariol monomethyl ether were isolated from the ethyl acetate extract of the fungus, CPLE-3. Conclusion: Bioactivity and chemical screening of the endophytic fungal extracts of Carica papaya leaf along with the isolation of fungal secondary metabolites suggested these endophytic fungi to be possible source of bioactive leads for developing new and improved medicines.

Keywords


Alternaria, Alternariol Monomethyl Ether, Carvularia, Colletotrichum, Ergosterol

References