Journal of Natural Remedies

Open Access Open Access  Restricted Access Subscription Access

Analyzing the Phytochemical, Anti-Ulcer, Anthelmentic and Antioxidant Potentials of Tabernaemontana dichotoma Roxb. ex Wall Seed Extracts


Affiliations
1 Department of Studies in Bioscience, University of Mysore, Hassan, Karnataka, India
2 Department of Biochemistry, Government Science College, Hassan – 573201, Karnataka, India
 

In the current investigation, phytochemical analysis, antioxidant, anti-ulcer and in vitro anthelmintic studies of Tabernaemontana dichotoma Methanol Extract (TCME) and Aqueous Extract (TCAE) from seeds were evaluated. Phytochemical analysis confirmed the occurrence of various secondary metabolites. Biological evaluation showed a dose dependent response of different extracts of T. dichotoma seeds. Both the extracts showed antioxidant potential. TCME displayed higher degree of H+ K+ ATPase on par with omeprazole, thus confirming the anti-ulcer potential of the plant. TCME also exhibited better wormicidal activity compared to the other extract in both paralysis of earthworms (Pheretima posthuma), and confirming its role as anthelmentic medicine. The study confirms the anti-ulcer and anthelmentic properties of T. dichotoma seed extracts, with TCME showing higher efficacy.

Keywords

Antioxidant, Anti-Ulcer, DPPH, Phytochemicals.
Font Size

User

Notifications
JOURNAL COVERS
  

  • Narzary H, Swargiary A, Basumatary S. Proximate and vitamin C analysis of wild edible plants consumed by Bodos of Assam, India. Journal of Molecular Pathophysiology. 2015; 4(4):128–33.

  • Cerutti PA. 1991. Oxidative stress and carcinogenesis. Eur J Clin Invest. 21:1-11. https://doi.org/10.1111/j.1365-2362.1991.tb01350.xPMid:1907547

  • Wichi HP. Enhanced tumor development of butylated hydroxyanisol (BHA) from the prospective of effect on fore stomach and oesophageal squamous epithlium. Food and Chemical Toxicology. 1988; 26:717–23. https://doi.org/10.1016/0278-6915(88)90072-5

  • Williams RJ, Spencer JP, Rice-Evans C. Flavonoids: Antioxidants or signalling molecules? Free Radical Biology and Medicine. 2004 Apr 1; 36(7):838–49. https://doi.org/10.1016/j.freeradbiomed.2004.01.001 PMid:15019969

  • Borneo R, León AE, Aguirre A, Ribotta P, Cantero JJ. Antioxidant capacity of medicinal plants from the Province of Córdoba (Argentina) and their in vitro testing in a model food system. Food Chemistry. 2009 Feb 1; 112(3):664–70. https://doi.org/10.1016/j.foodchem.2008.06.027

  • Jain S, Jain A, Jain N, Jain DK, Balekar N. Phytochemical investigation and evaluation of in vitro free radical scavenging activity of Tabernaemontana divaricata Linn. Natural Product Research. 2010 Feb 15; 24(3):300–4. https://doi.org/10.1080/14786410903237123. PMid:20140809

  • Puranik SI, Hiremath MB, Nerli RB, Ghagane SC. Evaluation of in vitro antioxidant and anticancer activity of tabernaemontana divaricata leaf extracts Against T-24 human bladder cancer cell lines. International Journal of Cancer Research. 2018; 14:100–8. https://doi.org/10.3923/ijcr.2018.100.108

  • Qazzaz ME, Raja VJ, Lim KH, Kam TS, Lee JB, Gershkovich P, Bradshaw TD. In vitro anticancer properties and biological evaluation of novel natural alkaloid jerantinine B. Cancer Letters. 2016 Jan 28; 370(2):185–97. https://doi.org/10.1016/j.canlet.2015.10.013. PMid:26515390

  • Nicola C, Salvador M, Escalona Gower A, Moura S, Echeverrigaray S. Chemical constituents antioxidant and anticholinesterasic activity of Tabernaemontana catharinensis. The Scientific World Journal. 2013. https://doi.org/10.1155/2013/519858. PMid:23983637 PMCid:PMC3745974

  • Singleton VL, Orthofer R, Lamuela-Raventós RM. [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology. 1999 Jan 1; 299:152–78. https://doi.org/10.1016/S0076-6879(99)99017-1

  • Ghagane SC, Puranik SI, Kumbar VM, Nerli RB, Jalalpure SS, Hiremath MB, et al. In vitro antioxidant and anticancer activity of Leeaindica leaf extracts on human prostate cancer cell lines. Integrative Medicine Research. 2017 Mar 1; 6(1):79–87. https://doi.org/10.1016/j.imr.2017.01.004. PMid:28462147 PMCid:PMC5395687

  • Kukongviriyapan U, Luangaram S, Leekhaosoong K, Kukongviriyapan V, Preeprame S. Antioxidant and vascular protective activities of Cratoxylum formosum, Syzygium gratum and Limnophila aromatica. Biological and Pharmaceutical Bulletin. 2007; 30(4):661–6. https://doi.org/10.1248/bpb.30.661 PMid:17409498

  • Dash GK, Suresh P, Sahu SK, Kar DM, Ganapaty S, Panda SB. Evaluation of Evolvulus alsinoides Linn. for anthelmintic and antimicrobial activities. Journal of Natural Remedies. 2002 Jun 1; 2(2):182–5.

  • Rojas TR, Bourdy G, Ruiz E, Cerapio JP, Pineau P, Gardon J, Doimi F, Deparis X, Deharo E, Bertani S. Herbal medicine practices of patients with liver cancer in Peru: A comprehensive study toward integrative cancer management. Integrative Cancer Therapies. 2018 Mar; 17(1):52–64. https://doi.org/10.1177/1534735416681642. PMid:28088871PM Cid:PMC5950939

  • Jainu M, Devi CS. Antiulcerogenic and ulcer healing effects of Solanumnigrum (L.) on experimental ulcer models: Possible mechanism for the inhibition of acid formation. Journal of Ethnopharmacology. 2006 Mar 8; 104(1-2):156–63. https://doi.org/10.1016/j.jep.2005.08.064. PMid:16202548

  • Sen S, Biplab De ND, Chakraborty R. Anthelmintic and in vitro antioxidant evaluation of fractions of methanol extract of Leea asiatica leaves. Ancient Science of Life. 2012 Jan; 31(3):101. https://doi.org/10.4103/0257-7941.103184. PMid:23284215 PMCid:PMC3530333

  • Brand-Williams W, Cuvelier ME, Berset CL. Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology. 1995 Jan 1; 28(1):25–30. https://doi.org/10.1016/S0023-6438(95)80008-5

  • Naik Y, Jayaram S, Nayaka MH, Dharmesh SM. Gastroprotective effect of swallow ischolar_main (Decalepis hamiltonii) extract: Possible involvement of H+-K+-ATPase inhibition and antioxidative mechanism. Journal of Ethnopharmacology. 2007 May 30; 112(1):173–9. https://doi.org/10.1016/j.jep.2007.02.021 PMid:17395413

  • Mali RG, Wadekar RR. In vitro anthelmintic activity of Baliospermum montanum Muell. arg ischolar_mains. Indian Journal of Pharmaceutical Sciences. 2008 Jan; 70(1):131. https://doi.org/10.4103/0250-474X.40352. PMid:20390101 PMCid: PMC2852054

  • Chandrashekhar CH, Latha KP, Vagdevi HM, Vaidya VP. Anthelmintic activity of the crude extracts of Ficusracemosa. International Journal of Green Pharmacy. 2008; 2(2). https://doi.org/10.4103/0973-8258.41180


Abstract Views: 293

PDF Views: 203




  • Analyzing the Phytochemical, Anti-Ulcer, Anthelmentic and Antioxidant Potentials of Tabernaemontana dichotoma Roxb. ex Wall Seed Extracts

Abstract Views: 293  |  PDF Views: 203

Authors

P. Vishwaprakash Mahadimane
Department of Studies in Bioscience, University of Mysore, Hassan, Karnataka, India
S. P. Sharath Chandra
Department of Biochemistry, Government Science College, Hassan – 573201, Karnataka, India

Abstract


In the current investigation, phytochemical analysis, antioxidant, anti-ulcer and in vitro anthelmintic studies of Tabernaemontana dichotoma Methanol Extract (TCME) and Aqueous Extract (TCAE) from seeds were evaluated. Phytochemical analysis confirmed the occurrence of various secondary metabolites. Biological evaluation showed a dose dependent response of different extracts of T. dichotoma seeds. Both the extracts showed antioxidant potential. TCME displayed higher degree of H+ K+ ATPase on par with omeprazole, thus confirming the anti-ulcer potential of the plant. TCME also exhibited better wormicidal activity compared to the other extract in both paralysis of earthworms (Pheretima posthuma), and confirming its role as anthelmentic medicine. The study confirms the anti-ulcer and anthelmentic properties of T. dichotoma seed extracts, with TCME showing higher efficacy.

Keywords


Antioxidant, Anti-Ulcer, DPPH, Phytochemicals.

References





DOI: https://doi.org/10.18311/jnr%2F2020%2F24346