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Pimpinella alpina Molk Administration is Capable of Increasing Antioxidant and Decreasing Prooxidant Level Following UVB Irradiation


Affiliations
1 Department of Biochemistry, Sultan Agung Islamic University, Semarang, Indonesia
2 Department of Chemistry, Sultan Agung Islamic University, Semarang, Indonesia
 

Introduction: Indonesian male population has traditionally used Pimpinella alpina Molk (PaM) to prevent degenerative disease. However, the scientific evidence of PaM effect on increase in antioxidant and decrease in prooxidant level and their negative correlation remains unclear. Objective: To prove the effect of PaM on increase in antioxidant and decrease in prooxidant and their negative correlation following Ultraviolet B (UVB) exposed repeatedly. Methods: Forty male rats were assigned into 8 groups, treatment groups for 7 days: PaM 50 mg (PaM50-7), PaM 100 mg (PaM100-7), PaM 150 mg (PaM150-7), and for 15 days: PaM 100 mg (PaM100-15), PaM 150 mg (PaM150-15). The increase in antoxidant and decrease in prooxidant levels were measured by ELISA and Spectophotometer. Results: Statistical analysis indicated that antioxidant Catalase (CAT) and Super Oxyde Dismutase (SOD) activities in PaM groups were significantly higher, p < 0.001. In contrary, prooxidant levels marked by Malondialdehide (MDA) and 8-hydroxy-2-deoxyguanosine (8OHdG) concentrations in PaM groups were significantly lower, p < 0.001. There was also a negative correlation between antioxidant and prooxidant levels, p < 0.001. Conclusion: PaM administration with 50-150 mg daily dosage for 7-15 days capable of increasing antioxidant and decreasing Prooxidant levels, with a negative correlation following UVB irradiation repeatedly.

Keywords

Oxidative Stress, Pimpinella alpina Molk, CAT, MDA, SOD, 8OHdG.
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  • Pimpinella alpina Molk Administration is Capable of Increasing Antioxidant and Decreasing Prooxidant Level Following UVB Irradiation

Abstract Views: 381  |  PDF Views: 149

Authors

Taufiqurrachman Nasihun
Department of Biochemistry, Sultan Agung Islamic University, Semarang, Indonesia
Eni Widayati
Department of Chemistry, Sultan Agung Islamic University, Semarang, Indonesia

Abstract


Introduction: Indonesian male population has traditionally used Pimpinella alpina Molk (PaM) to prevent degenerative disease. However, the scientific evidence of PaM effect on increase in antioxidant and decrease in prooxidant level and their negative correlation remains unclear. Objective: To prove the effect of PaM on increase in antioxidant and decrease in prooxidant and their negative correlation following Ultraviolet B (UVB) exposed repeatedly. Methods: Forty male rats were assigned into 8 groups, treatment groups for 7 days: PaM 50 mg (PaM50-7), PaM 100 mg (PaM100-7), PaM 150 mg (PaM150-7), and for 15 days: PaM 100 mg (PaM100-15), PaM 150 mg (PaM150-15). The increase in antoxidant and decrease in prooxidant levels were measured by ELISA and Spectophotometer. Results: Statistical analysis indicated that antioxidant Catalase (CAT) and Super Oxyde Dismutase (SOD) activities in PaM groups were significantly higher, p < 0.001. In contrary, prooxidant levels marked by Malondialdehide (MDA) and 8-hydroxy-2-deoxyguanosine (8OHdG) concentrations in PaM groups were significantly lower, p < 0.001. There was also a negative correlation between antioxidant and prooxidant levels, p < 0.001. Conclusion: PaM administration with 50-150 mg daily dosage for 7-15 days capable of increasing antioxidant and decreasing Prooxidant levels, with a negative correlation following UVB irradiation repeatedly.

Keywords


Oxidative Stress, Pimpinella alpina Molk, CAT, MDA, SOD, 8OHdG.

References





DOI: https://doi.org/10.18311/jnr%2F2018%2F20187