Journal of Biological Control

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In vitro studies showed that 67.4 percent reduction of sclerotlal production in Sclerotium rolfsii was observed in the presence of Trichoderma viride Pers. (isolate 2). Mature sclerotia from each dual culture plate measured 647, 777, and 763 μm with T. viride (isolate 3), T. harzianum Rifai (isolate2) and Laetisaria aravalis Burdusall respectively. In slide germination test, 68.3 and 56.7 percent reduction of sclerotial germination was observed with T. viride (isolate 3) and T. harzianum (Isolate 2), respectively; whereas when plated in solid media, it was 47.5 percent with T. viride (isolate 3). The number of hyphae put forth by each sclerotium was found to be minimum in sclerotia produced in dual culture with T. viride (isolates 2 and 3).

Keywords

Trichoderma viride, T. harzianum, Laetisaria arvalis, Dual Culture, Incubation, Germination.
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