Journal of Biological Control

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Five different isolates of Pseudomonas fluorescens Migula were isolated from cowpea rhizosphere region and identified by biochemical tests. These strains were screened against Macrophomina phaseolina (Tassi) Goid. the causal organism of cowpea ischolar_main-rot and the results revealed that SVPF2 isolate recorded maximum inhibition of mycelial growth against control. The mechanism of PGPR viz. iron-chelating agent (siderophore) and volatiles (HCN) production tests were studied and SVPF2 isolate positively reacted for siderophore and HCN production. The talc-based formulation of SVPF2 was prepared and the bioefficacy was tested under green house conditions. The seed and soil application of talc-based formulation of SVPF2 significantly reduced the ischolar_main-rot incidence. The growth parameter viz. germination percentage and the vigour index were also increased in SVPF2 treated seeds.

Keywords

Cowpea, Pseudomonas fluorescent, Root-Rot, Rhizosphere, Siderophore.
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