Journal of Biological Control

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Grape berries were treated with different biological agents to test their efficacy in reducing post harvest incidence of black mould rot and minimize the loss. The agents Pseudomonas, Bacillus, Trichoderma and yeast isolates were individually screened against the black rot pathogen Aspergillus carbonarius. B. subtilis strains EPC-8 and EPCO-16 and T. harzianum (Th Co) showed high mycelial growth suppression of A. carbonarius in vitro. The effective biological control agents were tested in pre, post and combined inoculation studies against A. carbonarius in grape berries. In the pre inoculation, B. subtilis (EPC-8) showed 57.80 per cent reduction in the incidence of A. carbonarius followed by T. harzianum (Th Co) (48.43 percent). The same trend of effectiveness was also found in the post-inoculation and combined inoculation tests under storage conditions at room temperature in grape berries.

Keywords

Aspergillus carbonarius, Bacillus subtilis, Trichoderma harzianum.
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