Journal of Biological Control

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Investigation on the bio-efficacy of fungal and bacterial bio-agents against Alternaria alternata inciting little millet leaf blight


Affiliations
1 Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bangalore –560065, Karnataka, India
2 PC Unit, ICAR-AICRP on Small millets, ZARS, UAS, GKVK, Bangalore – 560065, Karnataka, India
 

 Little millet (Panicum sumatrense Roth ex Roem. and Schult.) is one of the hardiest minor cereal crop indigenous to Indian sub-continent. The crop is cultivated by tribal and poor farmers for food and feed. Leaf blight disease has been a major production constraint and fungicidal sprays for the management of the disease may not be economically viable and feasible. Hence, the present in vitro study was carried out to know the antifungal activity of six fungal and 10 bacterial bio-agents against Alternaria alternata inciting little millet leaf blight disease. Among the fungal bio-agents, Trichoderma harzianum (ThB5) and among the bacterial bio-agents, Bacillus velezensis (P42) showed mycelial growth inhibition of 75.18 and 84.75%, respectively.


Keywords

Alternaria alternata, Bacillus velezensis, biocontrol, little millet, Trichoderma harzianum
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  • Babu S, Seetharaman K, Nandakumar R, Johnson I. 2000. Biocontrol efficacy of Pseudomonas fluorescens against Alternaria solani on tomato leaf blight disease. Ann Pl Prot Sci. 8:252–4.

  • Bhat S, Nandini C, Tippeswamy V, Prabhakar. 2018. Significance of small millets in nutrition and health -A review. Asi. J. Dairy and Food Res. 37(1): 35–40. https://doi.org/10.18805/ajdfr.DR-1306

  • Changmei S, Dorothy, J. 2014. Millet - the frugal grain. Int. J. of Sci. Res. and Revi. 3(4):75–90.

  • Chauhan SS. 2014. Studies on banded leaf and sheath blight of little millet (Panicum sumatrense) caused by Rhizoctonia solani Kuhn. [M.Sc. (Agri.)] thesis. J.N.K.V.V. Jabalpur (MP). India. p. 1–95.

  • Chethana BS, Ganeshan G, Rao AS, Bellishree K. 2012, In vitro evaluation of plant extracts, bio-agents and fungicides against Alternaria porri (Ellis) Cif. causing purple blotch disease of onion. Pest Manag Hort Eco.

  • (2): 194–8.

  • Dai Z, Shuiqing Y, Dongmei Z, Jinglin Z, Yang P, Yiqing Y, Zhihui Y, Jiehua Z, Ya Z, Ran I. 2020. Inhibitory effects of non-volatiles lipopeptides and volatiles ketones metabolites secreted by Bacillus velezensis C16 against Alternaria solani. Biol. Control. 152:104421. https://doi.org/10.1016/j.biocontrol.2020.104421

  • Dragana J, Katarin P, Mira S, Sasa S, Snezana P, Miladinovic M, Svetlana R. 2012. Phenazines producing

  • Pseudomonas isolates decrease Alternaria tenuissima growth, pathogenicity and disease incidence on cardoon. Arch Biol Sci. 64(4):1495–503. https://doi.org/10.2298/ ABS1204495J

  • Elad Y. 2000. Biological control of foliar pathogens by means of Trichoderma harzianum and potential modes of action. Crop Prot. 19:709–14. https://doi.org/10.1016/ S0261-2194(00)00094-6

  • Ghaffar A. 1969. Biological control of white rot of onion. Interaction effect of soil microorganisms with Sclerotium cepivorum Berk J Mycol Plant Pathol. 38:101–11. https://doi.org/10.1007/BF02051680

  • Hariprasad K, Nagaraja A, Patil S, Rakesha. 2018. In vitro evaluation of plant extracts and bioagents against Alternaria tenuissima (Fr.) keissl causing leaf blight of kodo millet. Int J Chem Stud. 6(2): 504–7.

  • Kgatle MG, Truter M, Ramusi TM, Flett B, Aveling TAS. 2018. Alternaria alternata, the causal agent of leaf blight of sunflower in South Africa. Eur J Plant Pathol. 151:677–88.

  • https://doi.org/10.1007/s10658-017-1402-7

  • Kiran Babu T, Thakur RP, Upadhyaya, HD, Reddy PN, Sharma R, Girish, AGM, Sarma NDRK. 2013. Resistance to blast (Magnaporthe grisea) in a mini-core collection of finger millet germplasm. Eur J Plant Pathol. 135:299– 311. https://doi.org/10.1007/s10658-012-0086-2

  • Konstantinova PP, Bonants JM, Van Gent-pelzer, M, Van der zouwen P, Van der bulk R. 2002. Development of specific primers for detection and identification of Alternaria spp. in carrot material by PCR and comparison with blotter and plating assays. Myco. Res. 106:23–33.

  • https://doi.org/10.1017/S0953756201005160

  • Monte E. 2001. Understanding Trichoderma: Between biotechnology and microbial ecology. Int Microbiol. 4:1–4.

  • Murray MG, Thompson WF. 1980. Rapid isolation of high molecular weight plant DNA. Nucelic Acids Res.

  • :4321–6. PMid: 7433111 PMCid: PMC324241. https://doi.org/10.1093/nar/8.19.4321

  • Priyanka R, Nakkeeran S, Arumuka Pravin I, Krishna moorthy AS, Sivakumar U. 2018. Antifungal activity of Bacillus subtilis subsp. spizizenii (MM19) for the management of Alternaria leaf blight of marigold. J. Biol Control. 32(2): 95–102. https://doi.org/10.18311/jbc/2018/21134

  • Rao BD, Bhaskarachary K, Christina GDA, Devi GS, Tonapi VA. 2017. Nutritional and health benefits of millets. ICAR Indian Institute of Millets Research (IIMR) Rajendranagar. 112:1–150.

  • Regassa AB. 2020. Enhancing resistance of Sesamum indicum against Alternaria sesami through Bacillus velezensis AR1. (wileyonlinelibrary.com).

  • Silo-Suh LA, Lethbridge BJ, Raffel SJ, He HY, Clardy J, Handelsman J. 1994. Biological activities of two fungistatic antibiotics produced by Bacillus cereus Uw85. Appl Environ Microbiol. 60:2023-2030. PMid: 8031096 PMCid: PMC201597. https://doi.org/10.1128/AEM.60.6.2023-2030.1994

  • Simmons EG. 2007. Alternaria: An identification manual. Utretch, The Netherlands: Central blatt for Schimmel cultures. p. 775.

  • Thaware DS, Fugro PA, Jadhav YT, Magar SV, Karande RA. 2011. In vitro evaluation of different fungicides, plant extracts and bio-agents against Alternaria alternata causing leaf blight of cowpea (Vigna unguiculata).

  • Green Farming. 2(5):563–6.

  • Tutte J. 1969. Plant pathological methods fungi and bacteria. USA: Burgess Publishing Company; p. 229.

  • Vinale F, Sivasithamparam K, Ghisalberti E, Marra R, Woo S, Lorito M. 2008. Trichoderma plant-pathogen interactions. Soil Biochem. 40(1):1–10. https://doi.org/10.1016/j.soilbio.2007.07.002

  • Vincent JM. 1947. Distribution of fungal hyphae in the presence of certain inhibitors. Nature. 159: 850. PMid: 20343980. https://doi.org/10.1038/159850b0

  • White TJ. 1990. PCR Protocols: A guide to methods and applications. San Diego, CA: Academic Press; p. 315.

  • Woudenberg JHC, Seidl MF, Groenewald JZ, De vries M, Stielow JB, Thomma BPHJ, Crous PW. 2015. Alternaria section Alternaria: Species, formae speciales or pathotypes? Studies In Mycology. 82:1–21. PMid: 26951037 PMCid: PMC4774270. https://doi.org/10.1016/j.simyco.2015.07.001

  • Zade SB, Ingle YV, Ingle RW. 2018. Evaluation of fungicides, botanicals and bio-agents against Alternaria alternata incitant of leaf spot of soybean. J Pharmacogn Phytochem. 7(5):1687–90.


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  • Investigation on the bio-efficacy of fungal and bacterial bio-agents against Alternaria alternata inciting little millet leaf blight

Abstract Views: 316  |  PDF Views: 135

Authors

Boda Praveen
Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bangalore –560065, Karnataka, India
K. B. Palanna
PC Unit, ICAR-AICRP on Small millets, ZARS, UAS, GKVK, Bangalore – 560065, Karnataka, India
M. K. Prasanna Kumar
Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bangalore –560065, Karnataka, India
A. Nagaraja
Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bangalore –560065, Karnataka, India

Abstract


 Little millet (Panicum sumatrense Roth ex Roem. and Schult.) is one of the hardiest minor cereal crop indigenous to Indian sub-continent. The crop is cultivated by tribal and poor farmers for food and feed. Leaf blight disease has been a major production constraint and fungicidal sprays for the management of the disease may not be economically viable and feasible. Hence, the present in vitro study was carried out to know the antifungal activity of six fungal and 10 bacterial bio-agents against Alternaria alternata inciting little millet leaf blight disease. Among the fungal bio-agents, Trichoderma harzianum (ThB5) and among the bacterial bio-agents, Bacillus velezensis (P42) showed mycelial growth inhibition of 75.18 and 84.75%, respectively.


Keywords


Alternaria alternata, Bacillus velezensis, biocontrol, little millet, Trichoderma harzianum

References





DOI: https://doi.org/10.18311/jbc%2F2020%2F26352