Journal of Biological Control

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Evaluation of Beauveria bassiana and Metarhizium anisopliae isolates against Plutella xylostella (L.) under laboratory conditions


Affiliations
1 ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H.A. Farm Post, Bellary Road, Bengaluru - 560024, Karnataka, India
2 Department of Biotechnology, Center for Post Graduate Studies, Jain University, Jayanagar, Bengaluru - 560011, Karnataka, India
 

Twenty isolates each of Beauveria bassiana and Metarhizium anisopliae were tested against the second instar larvae of Plutella xylostella through laboratory bioassay using larval dip method at the concentration of 1×107 conidia/ml. Higher larval mortality of 88.85% and 81.44%, were observed with NBAIR Ma-4 and NBAIR Ma-35 isolates of M. anisopliae and 77.36% with NBAIR-Bb-5a isolate of B. bassiana. Further studies on dose and time mortality with four isolates indicated lowest LC50 value of 2.6×104 conidia/ml and LT50 of 86.6 hours with NBAIR Ma-35 isolate. These isolates have to be further tested against P. xylostella in infested fields of cabbage and cauliflower.


Keywords

Beauveria bassiana, Entomopathogenic fungi, Metarhizium anisopliae, Plutella xylostella
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  • Evaluation of Beauveria bassiana and Metarhizium anisopliae isolates against Plutella xylostella (L.) under laboratory conditions

Abstract Views: 298  |  PDF Views: 121

Authors

Bathina Poornesha
ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H.A. Farm Post, Bellary Road, Bengaluru - 560024, Karnataka, India
Bonam Ramanujam
Department of Biotechnology, Center for Post Graduate Studies, Jain University, Jayanagar, Bengaluru - 560011, Karnataka, India

Abstract


Twenty isolates each of Beauveria bassiana and Metarhizium anisopliae were tested against the second instar larvae of Plutella xylostella through laboratory bioassay using larval dip method at the concentration of 1×107 conidia/ml. Higher larval mortality of 88.85% and 81.44%, were observed with NBAIR Ma-4 and NBAIR Ma-35 isolates of M. anisopliae and 77.36% with NBAIR-Bb-5a isolate of B. bassiana. Further studies on dose and time mortality with four isolates indicated lowest LC50 value of 2.6×104 conidia/ml and LT50 of 86.6 hours with NBAIR Ma-35 isolate. These isolates have to be further tested against P. xylostella in infested fields of cabbage and cauliflower.


Keywords


Beauveria bassiana, Entomopathogenic fungi, Metarhizium anisopliae, Plutella xylostella

References





DOI: https://doi.org/10.18311/jbc%2F2020%2F26131