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In Vitro Interaction of Pseudomonas Fluorescens with Phomopsis theae, the Causal Agent of Phomopsis Canker Disease in Tea Plants


Affiliations
1 1Research and Development Centre, Bharathiar University, Coimbatore–641046, Tamil Nadu, India
2 Department of Biotechnology, K.S. Rangasamy College of Technology, Tiruchengode–637215, Tamil Nadu, India
     

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An interaction between the Phomopsis canker pathogen Phomopsis theae and a bacterial antagonist like Pseudomonas fluorescens were studied under in vitro condition. Dual culture studies, morphological interaction and antibiosis revealed the antagonistic properties of the bacterial antagonist upon P. theae. Interaction studies on the tea stem bits further confirm this and also revealed that P. fluorescens were able to suppress the growth and reproduction of P. theae significantly. The results indicated the potential of this organism as a biocontrol agent against P. theae. The results on dual culture experiment showed that the growth of pathogen was completely inhibited within three days of inoculation with P. fluorescens. Similarly, the results on antibiosis experiment indicated that cent percent inhibition was recorded after two days of inoculation with P. fluorescens and among the two media tested for toxic metabolite production from P. fluorescens, gliotoxin fermentation medium was found to be better than nutrient broth in terms of cent percent inhibition of the pathogen and bioactive secondary metabolite production.

Keywords

Phomopsis canker, Phomopsis theae, Pseudomonas fluorescens, Antagonism, Biocontrol, In Vitro Interaction.
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Notifications

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  • In Vitro Interaction of Pseudomonas Fluorescens with Phomopsis theae, the Causal Agent of Phomopsis Canker Disease in Tea Plants

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Authors

V. Kanimozhi
1Research and Development Centre, Bharathiar University, Coimbatore–641046, Tamil Nadu, India
P. Ponmurugan
Department of Biotechnology, K.S. Rangasamy College of Technology, Tiruchengode–637215, Tamil Nadu, India

Abstract


An interaction between the Phomopsis canker pathogen Phomopsis theae and a bacterial antagonist like Pseudomonas fluorescens were studied under in vitro condition. Dual culture studies, morphological interaction and antibiosis revealed the antagonistic properties of the bacterial antagonist upon P. theae. Interaction studies on the tea stem bits further confirm this and also revealed that P. fluorescens were able to suppress the growth and reproduction of P. theae significantly. The results indicated the potential of this organism as a biocontrol agent against P. theae. The results on dual culture experiment showed that the growth of pathogen was completely inhibited within three days of inoculation with P. fluorescens. Similarly, the results on antibiosis experiment indicated that cent percent inhibition was recorded after two days of inoculation with P. fluorescens and among the two media tested for toxic metabolite production from P. fluorescens, gliotoxin fermentation medium was found to be better than nutrient broth in terms of cent percent inhibition of the pathogen and bioactive secondary metabolite production.

Keywords


Phomopsis canker, Phomopsis theae, Pseudomonas fluorescens, Antagonism, Biocontrol, In Vitro Interaction.

References