Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Effect of Metabolic Stress and Various Environmental Conditions on Lipid Accumulation in Halophilic Yeast Candida albicans


Affiliations
1 Loyola centre for research and development, Ahmedabad, Gujarat-380009, India
     

   Subscribe/Renew Journal


Candida albicans is one of the most commonly found yeast. It has capacity to accumulated higher quantity of lipids which can be used as SCO (single cell oil) or a feedstock for biodiesel production. In this experiment, an attempt was made to determine the effect of metabolic stress conditions and various environmental conditions on lipid accumulation capability of yeast. As an outcome of this experiment, it was found that ratio of C:N has great impact on lipid accumulation. Not only this, temperature and incubation time also plays a significant role in lipid accumulation. Under controlled condition Candida was successfully able to accumulate seven times higher lipids within the cells as compared to normal growth conditions.

Keywords

C:N ratio, Lipid Accumulation, Yeast
User
Notifications

  • Antoni, Dominik, Vladimir V Zverlov, and Wolfgang H Schwarz. 2007. “Biofuels from microbes.” Applied Microbiology and Biotechnolgy 77: 23-35.
  • Atlas, Ronald. 1993. Handbook of microbiological media. CRC Press.
  • Blagovi, Branka et al. 2001. “Lipid Composition of Brewer ’s Yeast.” Food Technology and Biotechnology 39(3): 175-181.
  • Brown, CM, and AH Rose. 1969. “Fatty-Acid Composition of Candida utilis as Affected by Growth Temperature and Dissolved- Oxygen Tension.” Journal of Bacteriology 99(2): 371-378.
  • Bump, Charles M, and Lawrence J Kunz. 1968. “Routine Identification of Yeasts with the Aid of Molybdate Agar Medium.” Applied Microbiology 16(10): 1503-1506.
  • Evans, Christopher, and Colin Ratledge. 1983. “A Comparison of the Oleaginous Yeast , Candida curvata , Grown on Different Carbon Sources in Continuous and Batch Culture.” Lipids 18(9): 623-629.
  • Gill, Colin O, Michael J Hall, and Colin Ratledge. 1977. “Lipid Accumulation in an Oleaginous Yeast (Candida 107) Growing on Glucose in Single-Stage Continuous Culture.” Applied and Environmental Microbiology 33(2): 231- 239.
  • Guarneri, J J, T J Combs, and M A Pisano. 1984. “Lipid Composition of Candida stellatoidea as Affected by Culture Age and Medium Aeration.” Annals New York Academy of Sciecnes 435: 595-597.
  • Hall, Michael J, and Colin Ratledge. 1977. “Lipid Accumulation in an Oleaginous Yeast (Candida 107) Growing on Glucose Under Various Conditions in a One- and Two-Stage Continuous Culture.” Applied and Environmental Microbiology 33(3): 577-584.
  • Hossack, By J A, A H Rose, and P S S Dawson. 1979. “Changes in the Lipid Composition of Candida utilis during the Cell Cycle.” Jounral of General Microbiology 113: 199-202.
  • Mago, N, and G K Khuller. 1990. “Lipids of Candida albicans􀀀: subcellular distribution and biosynthesis.” Journal of General Microbiology 136: 993-996.
  • Mahmoudabadi, A Zarei, and D B Drucker. 2006. “Comparison of polar lipids from yeast and mycelial forms of Candida albicans and Candida dubliniensis.” Mycoses 49: 18-22.
  • Müllner, Heidemarie, and Günther Daum. 2004. “Dynamics of neutral lipid storage in yeast” Acta Biochimica Polonica 51(2): 323-347.
  • Rale and Vakil. 1984. “A note on an improved molyhdate agar for the selective isolation of yeasts from tropical fruits.” Journal of Applied Bacteriology 56: 409-413.
  • Vakhlu, Jyoti, and Avneet Kour. 2006. “Yeast lipases􀀀: enzyme purification , biochemical properties and gene cloning.” Electronic Journal of Biotechnology 9(1): 69-85.
  • Zhao, Xin et al. 2008. “Medium optimization for lipid production through cofermentation of glucose and xylose by the oleaginous yeast Lipomyces starkeyi.” European Journal of Lipid Science and Technology 110: 405-412.

Abstract Views: 453

PDF Views: 2




  • Effect of Metabolic Stress and Various Environmental Conditions on Lipid Accumulation in Halophilic Yeast Candida albicans

Abstract Views: 453  |  PDF Views: 2

Authors

Hardik R. Gohel
Loyola centre for research and development, Ahmedabad, Gujarat-380009, India
Sandip K Ghosh
Loyola centre for research and development, Ahmedabad, Gujarat-380009, India
Vincent J. Braganza
Loyola centre for research and development, Ahmedabad, Gujarat-380009, India

Abstract


Candida albicans is one of the most commonly found yeast. It has capacity to accumulated higher quantity of lipids which can be used as SCO (single cell oil) or a feedstock for biodiesel production. In this experiment, an attempt was made to determine the effect of metabolic stress conditions and various environmental conditions on lipid accumulation capability of yeast. As an outcome of this experiment, it was found that ratio of C:N has great impact on lipid accumulation. Not only this, temperature and incubation time also plays a significant role in lipid accumulation. Under controlled condition Candida was successfully able to accumulate seven times higher lipids within the cells as compared to normal growth conditions.

Keywords


C:N ratio, Lipid Accumulation, Yeast

References