Screening of Beta-lactam Acylase Producers from Soil and Characterization of Isolates for Substrate Specificity for Cephalosporins

Trupti K. Ashar; V. W. Rajasekar; Anupama Datla

doi:10.17485/ijst/2015/v8i14/75230

Screening of Beta-lactam Acylase Producers from Soil and Characterization of Isolates for Substrate Specificity for Cephalosporins

Trupti K. Ashar ¹, V. W. Rajasekar ², Anupama Datla ²

Affiliations
1 Department of Management, AMET University, Chennai, Ghana
2 R&D Biotech Division, Fermenta Biotech Ltd, DIL Complex, Majiwada, Thane, 400610, India

Abstract
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Beta-lactam acylase enzyme producers were isolated from soil samples. Soil samples were induced with phenyl amino acid derivatives for selelctive isolation. Out of the two potential isolates obtained, one of the isolates was studied for presence of beta-lactam acylase enzyme with cephalexin as model substrate. Enzyme activity of isolate grown in media at varying pH showed it to be tolerant to alkaline pH 8.5. Also the substrate specificity for various cephalosporins showed it most selective to cephalexin.