Department of Agronomy, Faculty of Agriculture, University of Zabol

Stimulating Bacteria use to enhance the growth and development of plants. This study focuses on “The Effect of Application Type and Composition of Growth Stimulating Bacteria on Quantitative and Qualitative Characteristics of Medicinal Plant Calendula”. An experimental test was conducted as a factorial randomized complete block design with three replications at the Agricultural Research Institute of Zabol University. The first factor is types of growth stimulating bacteria in octahedral: control (no inoculation), Azotobacter chroococcum, Azosperilium brasilense, Pesudomonans putida, Azotobacter chroococcum plus Azosperilium brasilense, Pesudomonans putida plus Azotobacter chroococcum, Pesudomonans putida plus Azosperilium brasilense, Pesudomonans putida plus Azotobacter chroococcum plus Azosperilium brasilense and the second factor is the type of consumption including two levels (Seed and Foliar application). The results showed that growth stimulating bacteria and application type had a significant effect on all analyzed traits. Foliar traits on the flower yield, the flower number per square meter, ratios of total biomass to flowers, total biomass to grain yield, flower harvest index, flower extract yield and Seed application affected on grain yield, grain harvest index, oil percentage, and oil yield had the greatest impact. The foliar application treatment is better to increase the performance of quantitative characteristics and seed treatments be used to enhance the quality performance. Azospirillum + Pseudomonas treatment brought great impact on the qualitative and quantitative characteristics of Calendula.