Analysis of Saliva and Serum of Normal and Anomalies Pregnant Women - Folic Acid Deficiency using FTIR Spectroscopy

This study is an attempt to evaluate and compare the spectral difference in saliva and serum between healthy pregnant women and anomalies pregnant women due to folic acid deficiency, using Fourier Transform Infrared Spectroscopy. Folic acid is required for the development of a healthy fetus and plays an important role in the development of the fetus spinal cord and brain. The present work is to study the folic acid deficiency in pregnancy-Anomalies (open neural defect) and compare the results with the normal healthy pregnant women. The results showed that there is a significant difference between the folic acid of healthy pregnant and anomalies (open neural defect) in pregnant women both in saliva and serum sample. From the spectral study, the intensity ratio parameters R₁ (I₁₃₃₈/I₃₄₁₅) R₂ (I₃₅₄₆/I₁₆₃₆) R₃ (I₂₈₅₄/I₃₄₁₅) R₄(I₁₇₄₃/I₃₅₄₆) R₅ (I₁₄₈₂/I₁₅₁₁) R₆ (I₁₄₈₂/I₁₆₉₆) R₇ (I₁₁₃₅/I₁₆₀₇) R₈ (I₂₉₂₇/I₁₆₀₇) R₉ (I₂₉₂₇/I₁₆₀₄) R₁₀ (I₁₄₉₂/I₁₅₁₁) have been introduced and computed. The outcome of the results shows that FTIR can be used in the qualitative and quantitative investigation of biological fluids to distinguish sample sets from healthy and diseased groups. The internal standard method is adopted in characterizing the samples quantitatively.