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Antibacterial and Antioxidant Investigations of Hallea ledermannii Leaf Extract


Affiliations
1 Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Nigeria
2 Department of Clinical Pharmacy, Faculty of Pharmacy, University of Lagos, Nigeria
3 Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Lagos, Nigeria
 

Hallea ledermannii (Krause) Verdc. (Rubiaceae) is used for treatment of various microbial diseases such as dysentery and skin infections. The leaf extract was screened for antibacterial activities using agar cup plate method against pathogenic organisms like Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Klebseilla pneumonia. The extract was active against both Gram positive and Gram negative bacteria with MIC value of 50.0, 12.5, 50.0, 18.75 and 12.5 mg/ml respectively. The antioxidant activities of the extract were also determined using total phenolic content and DPPH radical scavenging effect. The content of phenolic compounds in terms of gallic acid was found to be 88.0 mg/g. and scavenging power was found to increase with concentration. These may be responsible for the observed antimicrobial properties of the extract thus justifying its use in traditional medicine for treatment of microbial infections.

Keywords

Antibacterial, Antioxidant, Medicinal Plants, Radical Scavenging Effect
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  • Adelheid B, Males Z, Pepeljnjak S and Antolic A (1996) Antimicrobial activity of Paliurus spinachristi Mills (Christ’s thorn). J. Ethnopharmacol. 52, 119- 122.

  • Adesegun SA and Rotibi MO (1998) Antibacterial activities of crude shrub extract of Heliotropium indicum. J. Phamaceut. Sci. & Pharm. Prac. 4, 7-9.

  • Adesegun SA, Fajana A, Orabueze CI and Coker HAB (2009) Evaluation of antioxidant properties of Phaulopsis fascisepala. Evid. Based Complement. Altern. Med. 6, 227-231.

  • Agnese AM, Perez C and Cabrera JL (2001) Adesmia aegicera: antimicrobial activity and chemical study. Phytomedicine. 8, 389-394.

  • Ahmed I and Beg AZ (2001) Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J. Ethnopharmacol. 74, 113-123

  • Baker FJ and Breach MR (1980) Medical microbiological techniques (1st ed).Butterworths, London.

  • Burkill HM (1997) The useful plants of West Tropical Africa. 2nd edn, vol. 4, Royal Botanical Garden, Kew. pp. 546-547.

  • Cowan MM (1999) Plant products as antimicrobial agents. Clin. Microbiol. Rev. 12, 564-582.

  • Cushine TPT and Lamb AJ (2005) Antimicrobial activity of flavonoids. Int. J. Antimicrob. Agents, 26, 343-356.

  • Field JA and Lettinga G (1992) Toxicity of tannic compounds to microorganisms. Plant Polyphenols: Synthesis, Properties, Significance. Basic Life Sci. 59, 673-692.

  • Haslam E (1996) Natural polyphenols (vegetable tannins) as drugs: possible mode of action. J. Natl. Prod. 59, 205-215.

  • Karaman I, Sahin F, Gulluce M, Ogutcu H, Sengul M and Adiguzel A (2003) Antimicrobial activity of aqueous and methanol extracts of Jupiperous onycedrus L. J. Ethnopharmacol. 85, 213-235.

  • Kokoska L, Polesny Z, Rada V, Nepovim A and Vanek T (2002) Screening of some Siberian medicinal plants for antimicrobial activity. J. Ethnopharmacol. 82, 51-53.

  • Mohanta TK, Patra JK, Rath SK, Pal DK and Thatoi HN (2007) Evaluation of antimicrobial activity and phytochemical screening of oils and nuts of Semicarpus anacardium. Sci. Res. Essay, 2, 486- 490.

  • Mokbel MS and Hashinaga F (2006) Evaluation of the antioxidant activity of extract from butane (Citrus grandis Osbeck) fruit tissues. Food Chem. 9,529-534.

  • Newman DJ, Cragg GM and Snader KM (2000) The influence of natural products upon drug discovery. Natl. Prod. Rep., 17, 175-185.

  • Rath CC, Dash SK and Mishra RK (2002) Antimicrobial efficacy of six Indian essential oils individually and in combinations. J. Essential Oil Bearing Plants, 5, 99-107.

  • Sanders CC and Sanders WE (1992) ß-lactamase resistance in Gram-negative bacteria: Global trends and clinical impact. Clin. Infect. Dis. 15, 824-839.

  • Scalbert A (1991) Antimicrobial properties of tannins. Phytochemistry. 30, 3875-3883.

  • Sofowora AE (1993) Screening Plants for Bioactive Agents. In: Medicinal plants and traditional medicine in Africa. (ed. Sofowora, AE), 2nd edn., pp. 289. Spectrum books Ltd., Ibadan, Nigeria.

  • Yen GC and Chen HY (1995) Antioxidant activity of various teas extracts in relation to their antimutagenicity. J. Agric. Food Chem. 43, 27-32.


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  • Antibacterial and Antioxidant Investigations of Hallea ledermannii Leaf Extract

Abstract Views: 414  |  PDF Views: 97

Authors

S. Adeleke Adesegun
Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Nigeria
N. Emmanuel Anyika
Department of Clinical Pharmacy, Faculty of Pharmacy, University of Lagos, Nigeria
T. Oluseyi Adekoya
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Lagos, Nigeria
S. Godwin Essien
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Lagos, Nigeria

Abstract


Hallea ledermannii (Krause) Verdc. (Rubiaceae) is used for treatment of various microbial diseases such as dysentery and skin infections. The leaf extract was screened for antibacterial activities using agar cup plate method against pathogenic organisms like Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Klebseilla pneumonia. The extract was active against both Gram positive and Gram negative bacteria with MIC value of 50.0, 12.5, 50.0, 18.75 and 12.5 mg/ml respectively. The antioxidant activities of the extract were also determined using total phenolic content and DPPH radical scavenging effect. The content of phenolic compounds in terms of gallic acid was found to be 88.0 mg/g. and scavenging power was found to increase with concentration. These may be responsible for the observed antimicrobial properties of the extract thus justifying its use in traditional medicine for treatment of microbial infections.

Keywords


Antibacterial, Antioxidant, Medicinal Plants, Radical Scavenging Effect

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DOI: https://doi.org/10.17485/ijst%2F2012%2Fv5i1%2F30950