Indian Journal of Science and Technology

Open Access Open Access  Restricted Access Subscription Access

Microsatellite Analysis of Kangayam Cattle (Bos indicus) of Tamilnadu


Affiliations
1 Dept. of Animal Genetics & Breeding, Madras Veterinary College, Chennai – 600 007, India
 

Assessment of genetic variability in Kangayam breed of cattle in Tamilnadu, South India was carried out using 25 bovine microsatellite markers. The mean number of alleles was 4.04 ± 0.09 with a range of 2 to 6 and the allele size ranged from 94 to 300 bp. The frequency distribution of alleles in the breed was from 0.0104 to 0.9167. The estimated heterozygosity was 0.6183 ± 0.01 and the PIC was 0.5628 ± 0.03. The overall mean within-population inbreeding estimate (FIS) value (- 0.084) suggested excess of heterozygotes in the population. In addition, higher PIC value indicated the scope for maintaining variation in the population and strategies to take meaningful conservation.

Keywords

Cattle, Kangayam, Microsatellites, PIC
User

  • Cominicini S, Leone P, Redaell I, De Giuli L and Zhang Y (1995) Characterization of bovine microsatellites by silver staining. J. Anim. Breed. Genet. 112, 415-440.

  • Dorji T, Hanotte O, Arbenz MO, Rage JE and Roder W (2003) Genetic diversity of indigenous cattle populations in Bhutan: implications for conservation. Asian-Aust. J. Anim. Sci. 16, 946-951.

  • FAO (2004) Secondary Guidelines for Development of National Farm Animal Genetic Resources Management Plans for global management of cattle genetic resources using reference microsatellites Global Projects for the Maintenance of Domestic Animal Genetic Diversity (MoDAD). Domain site: http://www.fao.org/dad-is/

  • Kandasamy N (2001) Kangayam breed of cattle: present status and management practices. In: Indigenous Cattle and Their Role in the New Millennium, Proceedings of the Workshop Organized by TANUVAS, IDA and NDRI on 24- 25 March at Erode, Tamilnadu, India.

  • Karthickeyan SMK, Saravanan R and Thangaraju P (2006) Characterization of Krishna Valley breed of cattle (Bos indicus) in south India using microsatellite markers. Livestock Research for Rural Development 18, Article no. 151. Domain site: http://www.cipav.org.co/lrrd/ lrrd18/11/kart18151.htm

  • Karthickeyan SMK, Sivaselvam SN, Selvam R, Raja TV, Rajendran, R and Thangaraju P (2007) Umblahery breed of cattle in South India: genetic assessment through microsatellite markers. Asian J. Anim. Vet. Adv. 2, 218-222.

  • Kemp SJ, Hishida O, Wambugu J, Rink A and Longeri ML (1995) A panel of polymorphic bovine, ovine and caprine microsatellite markers. Anim. Genet. 26, 299-306.

  • Miller SA, Dykes D and Polesky HF (1988) A simple salting out procedure for extraction of DNA from human nucleated cells. Nucleic Acids Res. 16, 1215.

  • Metta M, Kanginakudru S, Gudiseva N and Nagaraju J (2004) Genetic characterization of Indian cattle breeds, Ongole and Deoni (Bos indicus) using microsatellite markers - a preliminary study. BMC Genet. 5, 16 Domain site: http://www.biomedcentral. Com/1471-2156/5/16

  • Mukesh M, Sodhi M, Bhatia S and Mishra BP (2004) Genetic diversity of Indian native cattle breeds as analysed with 20 microsatellite loci. J. Anim. Breed. Genet. 121, 416-424.

  • Naveen Kumar S, Jayashankar MR, Nagaraja CS, Govindaiah MG, Saravanan R and Karthickeyan SMK (2006) Molecular characterization of Hallikar breed of cattle using microsatellite markers. Asian- Aust. J. Anim. Sci. 19, 622–626.

  • Prabhu MBH (2004) Phenotypic and Molecular Genetic Characterization of Amritmahal Cattle. M.V.Sc. Thesis, Univ. of Agri. Sci., Bangalore, India. pp: 62-90.

  • Selvi PM, Panandam JM, Yusoff K and Tan SG (2004) Molecular characterization of the Mafriwal dairy cattle of Malaysia using microsatellite markers. Asian- Aust. J. Anim. Sci. 17, 1366 -1368.

  • Sodhi M, Mukesh M, Mishra BP, Mitkari KR, Prakash B and Ahlawat SPS (2005) Evaluation of genetic differentiation in Bos indicus cattle breeds from Marathwada region of India using microsatellite polymorphism. Anim. Biotech. 16, 127-137.


Abstract Views: 347

PDF Views: 60




  • Microsatellite Analysis of Kangayam Cattle (Bos indicus) of Tamilnadu

Abstract Views: 347  |  PDF Views: 60

Authors

S. M. K. Karthickeyan
Dept. of Animal Genetics & Breeding, Madras Veterinary College, Chennai – 600 007, India
S. N. Sivaselvam
Dept. of Animal Genetics & Breeding, Madras Veterinary College, Chennai – 600 007, India
R. Selvam
Dept. of Animal Genetics & Breeding, Madras Veterinary College, Chennai – 600 007, India
P. Thangaraju
Dept. of Animal Genetics & Breeding, Madras Veterinary College, Chennai – 600 007, India

Abstract


Assessment of genetic variability in Kangayam breed of cattle in Tamilnadu, South India was carried out using 25 bovine microsatellite markers. The mean number of alleles was 4.04 ± 0.09 with a range of 2 to 6 and the allele size ranged from 94 to 300 bp. The frequency distribution of alleles in the breed was from 0.0104 to 0.9167. The estimated heterozygosity was 0.6183 ± 0.01 and the PIC was 0.5628 ± 0.03. The overall mean within-population inbreeding estimate (FIS) value (- 0.084) suggested excess of heterozygotes in the population. In addition, higher PIC value indicated the scope for maintaining variation in the population and strategies to take meaningful conservation.

Keywords


Cattle, Kangayam, Microsatellites, PIC

References





DOI: https://doi.org/10.17485/ijst%2F2009%2Fv2i10%2F30717