Indian Journal of Science and Technology

Open Access Open Access  Restricted Access Subscription Access

Development of Molecularly Imprinted Polymers (MIPs) Nano Particles for the Selective Determination of Carbamazepine in Human Serum and Plasma


Affiliations
1 Food and Drug Laboratories Research Center, Ministry of Health and Medical Education, Tehran, Iran, Islamic Republic of
2 Food and Drug Laboratories Research Center, Ministry of Health and Medical Education, Semnan, Iran, Islamic Republic of
 

A new, simple, rapid, and sensitive solid-phase extraction with molecularly imprinted polymer (SPE-MIP) for determination of Carbamazepine in biological samples was developed. These nano particle polymers were synthesized via a non-covalent molecular imprinting approach through precipitation polymerization method using methacrylic acid (MAA) as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a cross-linker agent, carbamazepine as a target template molecule and azobisisobutyronitrile (AIBN) as an initiator. The optimal conditions for SPE consisted of conditioning the cartridge using a pH=3.0 water, loading 5.0 ml of the sample under basic aqueous conditions, clean-up using 2×2ml acetonitrile and elution with 3.0 ml methanol. After optimization of SPE procedure, an aliquot of extracted template was injected to the ACE, 5μm 250×4.6 mm analytical column with the mobile phase as the same as elution solvent. This method was used for extraction of carbamazepine from an anticonvulsant aqueous solution and the results revealed an extraction recovery of more than 90%. HPLC chromatograms show an efficient clean up, which supports the potential of MISPE for clean-up of trace amounts of carbamazepine from the drug formulation.

Keywords

Molecularly Imprinted Polymerl, Carbamazepine, Solid-phase Extraction, Biological Samples
User

  • Beghi (2002) Carbamazepine: clinical efficacy and use in other neurological disorders. In: levy R, Mattson R, Meldrum B, eds Antiepileptic drugs, 5th edn, Lippincott Williams & Wilkins, Philadelphia, pp: 273-277.

  • Bertilsson L and Tomson T (1986) Clinical pharmacokinetics and pharmacology effects of carbamazepine & carbamazepin-10, 11- epoxide: an update. Clin Pharmacokinet. 11, 177-198.

  • Goodwin FK and Jamison KR (1990) Biochemical and pharmacological studies. In: Goodwim FK, Jamison KR. eds Manic - Depressive Illness, Oxford university press, New York, pp: 416- 502 .

  • Janicak PG, Davis JM, Preskorn SH and Ayd FJ (1997) Treatment with mood stabilizers. In: Principles and Practice of Psychotherapy. Janicak PG, Davis JM, Preskon SH. eds, 2nd edn, Williams & Wilkins, Baltimore, pp. 403 –473.

  • Nathan KL, Musselman DL, Schatzberg AF and Nemeroff CB (1995) Biology of mood disorders. In: Text book of psychopharmacology. Schatzberg AF, Nemeroff CB. eds, American Psychiatric Press, Washington, pp: 439 - 477.

  • Trimble MR (2002) Carbamazepine: clinical efficacy and use in psychiatric disorders. In: Antiepileptic drugs. Levy R, Mattson R, Meldrum B, eds., 5th edn, Lippincott Williams & Wilkins, Philadelphia, pp: 278- 284.

  • Wyllie EE (2001) The treatment of epilepsy. 3rd edn, Lippincott Williams & Wilkins, Philadelphia, pp: 821- 835.


Abstract Views: 443

PDF Views: 120




  • Development of Molecularly Imprinted Polymers (MIPs) Nano Particles for the Selective Determination of Carbamazepine in Human Serum and Plasma

Abstract Views: 443  |  PDF Views: 120

Authors

Behrouz Akbari-adergani
Food and Drug Laboratories Research Center, Ministry of Health and Medical Education, Tehran, Iran, Islamic Republic of
Ahmad Heydarieh
Food and Drug Laboratories Research Center, Ministry of Health and Medical Education, Semnan, Iran, Islamic Republic of

Abstract


A new, simple, rapid, and sensitive solid-phase extraction with molecularly imprinted polymer (SPE-MIP) for determination of Carbamazepine in biological samples was developed. These nano particle polymers were synthesized via a non-covalent molecular imprinting approach through precipitation polymerization method using methacrylic acid (MAA) as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a cross-linker agent, carbamazepine as a target template molecule and azobisisobutyronitrile (AIBN) as an initiator. The optimal conditions for SPE consisted of conditioning the cartridge using a pH=3.0 water, loading 5.0 ml of the sample under basic aqueous conditions, clean-up using 2×2ml acetonitrile and elution with 3.0 ml methanol. After optimization of SPE procedure, an aliquot of extracted template was injected to the ACE, 5μm 250×4.6 mm analytical column with the mobile phase as the same as elution solvent. This method was used for extraction of carbamazepine from an anticonvulsant aqueous solution and the results revealed an extraction recovery of more than 90%. HPLC chromatograms show an efficient clean up, which supports the potential of MISPE for clean-up of trace amounts of carbamazepine from the drug formulation.

Keywords


Molecularly Imprinted Polymerl, Carbamazepine, Solid-phase Extraction, Biological Samples

References





DOI: https://doi.org/10.17485/ijst%2F2012%2Fv5iS3%2F30404