Indian Journal of Science and Technology

Open Access Open Access  Restricted Access Subscription Access

Identification of DNA Elements Involved in Somaclonal Variants of Rauvolfia Serpentina (L.) Arising from Indirect Organogenesis as Evaluated by ISSR Analysis


Affiliations
1 Post-graduate & Research Dept. of Plant biology and Biotechnology, Pachaiyappa’s College, Chennai-600030, India
2 Post-graduate & Research Dept. of Plant biology and Biotechnology, Presidency College, Chennai-600005, India
3 M.S.Swaminathan Research Foundation, Chennai-600 113, India
 

Rauvolfia serpentina an endangered medicinal plant was chosen for in vitro propagation using modified MS medium. Genetic fidelity study of the regenerated plants were analysed with 18 ISSR markers. A total of 159 monomorphic bands were obtained; one ISSR marker HB-12 showed a polymorphic band among the callus regenerants. The specific polymorphic fragment was then gel eluted, cloned and sequenced.

Keywords

Rauvolfia serpentina, ISSR, Somaclonal variation, Medicinal plant, India
User

  • Ahuja MR (1987) In vitro propagation of poplar and aspen. In: Cell and tissue culture in forestry. Bonga JM & Durzan DJ (eds) Vol 3. Martinus Nijhof, Dordrecht, pp: 207– 223.

  • Damasco OP, Graham GC, Henry RJ, Adkins SW and Godwin ID (1996) Random amplified polymorphic DNA (RAPD) detection of dwarf off-types in micropropagated Cavendish (Musa spp.AAA) bananas. Plant Cell Rep. 16,118-1123.

  • Goel MK, Mehrotra S, Kukreja AK, Shanker K and Khanuja SPS (2009) In vitro propagation of Rauwolfia serpentina using liquid medium, assessment of genetic fidelity of micropropagated plants, and simultaneous quantitation of reserpine, ajmaline, and ajmalicine. Methods in Mol. Biol. Clifton Nj. 547, 17- 33.

  • Goto S, Thakur RC and Ishii K (1998) Determination of genetic stability in long– term micro propagated shoots of Pinus thunbergii parl. using RAPD markers. Plant Cell Rep.18, 193– 197.

  • Hashmi G, Huettel R, Meyer R, Krusberg L and Hammerschlag F (1997) RAPD analysis of somaclonal variants derived from embryo callus cultures of peach. Plant Cell Rep. 16, 624–627.

  • Iriondo JM and Perez C (1996) Somaclonal variation in Lavatera. In: Somaclonal variation in crop improvement. Bajaj YPS (Ed.), Vol II, Springer- Verlag, Berlin. pp:280-295.

  • Jain SM (1997) Somaclonal variation and mutagenesis for crop improvement. Maat- alouden tutkimuskuksen, Sirkka Immonen (Ed.). 18, 122–132.

  • Jain SM, Brar DS and Ahloowalia BS (Eds.) (1998) Somaclonal variation and induced mutation in crop improvement. Kluwer Academic Publ., UK.

  • Kaushal Bindiya and Kamlesh Kanwar (2003) RAPD markers for genetic analysis in micropropagated plants of Robinia pseudoacacia L. Euphytica 132, 41- 47.

  • Larkin PJ and Scowcroft SC (1981) Somaclonal variation- a novel source of variability from cell culture for plant improvement. Theor. Appl. Genet. 60,197– 214.

  • Maria Antonietta Palombi, Beatrice Lombardo and Emilia Caboni (2006) In vitro regeneration of wild pear (Pyrus pyraster Burgsd) clones tolerant to Fechlorosis and somaclonal variation analysis by RAPD markers. Plant cell reports, genetics and genomics. online date: Wednesday, 15th Nov.

  • Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 15, 473-497.

  • Nitish Kumar, Modi AR, Singh AS, Gajera BB, Patel AR and Patel MP (2010) Assessment of genetic fidelity of micropropagated date palm (Phoenix dactylifera L.) plants by RAPD and ISSR markers assay. Physiol. Mol. Biol. Plants.16 (2), 207-213.

  • Rani V, Parida A and Raina SN (1995) Random amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoides Marsh. Plant Cell Rep. 14, 459– 462.

  • Richa Bhatt, Mohd Arif, Gaur AK and Rao PB (2008) Rauwolfia serpentina: Protocol optimization for in vitro propagation. Afr. J. Biotechnol. 7 (23), 4265- 4268.

  • Rival A, Betrand L, Beale T, Combes MC, Trouslout P and Leshermes P (1998) Suitability of RAPD analysis for detection of somaclonal variation in oil palm (Elaeis guineenis Jacq.) Plant Breed. 117, 73- 76.

  • Rogers SO and Bendich AJ (1988) Extraction of DNA from plant tissues. In: Plant molecular biology. Manual A6. Kluwer Academic publishers, Dordrecht. pp:1-10.

  • Rout GR, Das P, Goel S and Raina SN (1998) Determination of genetic stability of micropropagated plants of ginger using Random Amplified Polymorphic DNA (RAPD) markers. Botanical Bull. Acad. Sinica. 39, 23-27.

  • Schneider S, Reustle G and Zyprian E (1996) Detection of somaclonal variation in grapevine regenerants from protoplast by RAPD– PCR. Vitis. 35, 99–100.

  • Shu QY, Liu GS, Qi DM, Chu CC, Liu J and Li HJ (2003) An effective method for axillary bud culture and RAPD analysis of cloned plants in tetraploid black locust. Plant Cell Rep. 22, 175-180.

  • Sihag RC and Nidhi Wadhwa (2011) Floral and reproductive biology of Sarpagandha Rauvolfia serpentina (Gentianales: Apocynaceae) in semi-arid environment of India. J. Threatened Taxa (JoTT) Short Commun. 3(1), 1432-1436.

  • Skirvin RM and Janick J (1976) Tissue culture – induced variation in scented Pleargonium spp. J. Am. Soc. Hort. Sci. 101, 281–290.

  • Skirvin RM, Nortonand M and McPheeters KD (1993) Somaclonal variation: has it proved useful for plant improvement. Acta Hort. 336, 333-340.

  • Tiwari S and Jatav DS (2008) Detection of somoclonal variations aamong micropropagated population of some important medicinal plants. Afr. J. Traditional, Complementary & Alternative Med. (AJTCAM), Abstracts of tongress on Medicinal and aromatic Plants, Cape Town, Nov.

  • Varshney A, Lakshmikumaran M, Srivastava PS and Dhawan V (2001) Establishment of genetic fidelity of in vitro raised Lilium bulblets through RAPD markers. Special Issue of In Vitro Cellu. Dev. Biol-Plant. 37(2), 227-231.

  • Veilleux RE and Johnson AAT (1998) Somaclonal variation: Molecular analysis, transformation, interaction, and utilization. Plant Breed Rev. 16, 229– 268.

  • Vendrame WA, Kochert G and Wetzstein HY (1999) AFLP analysis of variation in pecan somatic embryos. Plant Cell Rep. 18, 853–857.

  • Wang P-J and Charles A (1991) Micropropagation through meristem culture. In: High tech and micropropagation. Biotechnology in agriculture and forestry. Bajaj YPS (ed), Vol.17, Springer, BerlinHeidelberg, NY. pp: 32–52.


Abstract Views: 386

PDF Views: 162




  • Identification of DNA Elements Involved in Somaclonal Variants of Rauvolfia Serpentina (L.) Arising from Indirect Organogenesis as Evaluated by ISSR Analysis

Abstract Views: 386  |  PDF Views: 162

Authors

S. Saravanan
Post-graduate & Research Dept. of Plant biology and Biotechnology, Pachaiyappa’s College, Chennai-600030, India
R. Sarvesan
Post-graduate & Research Dept. of Plant biology and Biotechnology, Presidency College, Chennai-600005, India
M. S. Vinod
M.S.Swaminathan Research Foundation, Chennai-600 113, India

Abstract


Rauvolfia serpentina an endangered medicinal plant was chosen for in vitro propagation using modified MS medium. Genetic fidelity study of the regenerated plants were analysed with 18 ISSR markers. A total of 159 monomorphic bands were obtained; one ISSR marker HB-12 showed a polymorphic band among the callus regenerants. The specific polymorphic fragment was then gel eluted, cloned and sequenced.

Keywords


Rauvolfia serpentina, ISSR, Somaclonal variation, Medicinal plant, India

References





DOI: https://doi.org/10.17485/ijst%2F2011%2Fv4i10%2F30164