Indian Journal of Science and Technology

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Isolation and Characterization of Probiotic Bacillus Subtilis SK09 from Dairy Effluent


Affiliations
1 Department of Biotechnology, St. Joseph’s College of Engineering, Chennai-600119, India
2 Department of Chemical Engineering, Sathyabama University, Chennai-600119, India
 

Health promoting microorganisms such as probiotics are recently been used as food additive and therapeutic supplement especially as enhancer of prophylaxis and digestion. Here we report the isolation and characterization of a probiotic bacillus species, with the ability to ferment lactose, from dairy effluent. Biochemical test and 16S rRNA sequencing were done in order to establish the species and strain details. The species was found to be Bacillus subtilis SK09 with a unique ability to ferment lactose. The strain was also subjected to commonly available antibiotics for its antibiotic susceptibility for its probiotic credibility.

Keywords

Probiotic Bacteria, Bacillus subtilis, Lactose Intolerance, 16SrRNA Sequencing
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  • Isolation and Characterization of Probiotic Bacillus Subtilis SK09 from Dairy Effluent

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Authors

G. Sreekumar
Department of Biotechnology, St. Joseph’s College of Engineering, Chennai-600119, India
Soundarajan Krishnan
Department of Chemical Engineering, Sathyabama University, Chennai-600119, India

Abstract


Health promoting microorganisms such as probiotics are recently been used as food additive and therapeutic supplement especially as enhancer of prophylaxis and digestion. Here we report the isolation and characterization of a probiotic bacillus species, with the ability to ferment lactose, from dairy effluent. Biochemical test and 16S rRNA sequencing were done in order to establish the species and strain details. The species was found to be Bacillus subtilis SK09 with a unique ability to ferment lactose. The strain was also subjected to commonly available antibiotics for its antibiotic susceptibility for its probiotic credibility.

Keywords


Probiotic Bacteria, Bacillus subtilis, Lactose Intolerance, 16SrRNA Sequencing

References





DOI: https://doi.org/10.17485/ijst%2F2010%2Fv3i8%2F29898