Indian Journal of Science and Technology

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A Method for Small Scale Genomic DNA Isolation from Chickpea (Cicer arietinum L.) Suitable for Molecular Marker Analysis


Affiliations
1 B. R. Doshi School of Biosciences, Sardar Patel Maidan, Vadtal Road, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar-388120, Gujarat, India
 

We describe a DNA isolation procedure for chickpea (Cicer arietinum L.) which is rapid and less expensive without involving ultra centrifugation or column purification steps. DNA preparation obtained from the present study was essentially suitable for PCR analysis which is one of the key steps in crop improvement programme through marker development and genetic engineering techniques. The yield of DNA ranged from 0.595- 5.550 μg/ml and the purity ratio was between 1.025- 2.010 indicating minimum levels of contaminating metabolites. The present protocol offers as a reliable, and consistent DNA isolation method for chickpea that yields large amount of pure&intact DNA.

Keywords

DNA Isolation, Cicer arietinum, PCR Analysis
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  • A Method for Small Scale Genomic DNA Isolation from Chickpea (Cicer arietinum L.) Suitable for Molecular Marker Analysis

Abstract Views: 551  |  PDF Views: 121

Authors

Neha Joshi
B. R. Doshi School of Biosciences, Sardar Patel Maidan, Vadtal Road, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar-388120, Gujarat, India
Anamika Rawat
B. R. Doshi School of Biosciences, Sardar Patel Maidan, Vadtal Road, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar-388120, Gujarat, India
R. B. Subramanian
B. R. Doshi School of Biosciences, Sardar Patel Maidan, Vadtal Road, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar-388120, Gujarat, India
K. S. Rao
B. R. Doshi School of Biosciences, Sardar Patel Maidan, Vadtal Road, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar-388120, Gujarat, India

Abstract


We describe a DNA isolation procedure for chickpea (Cicer arietinum L.) which is rapid and less expensive without involving ultra centrifugation or column purification steps. DNA preparation obtained from the present study was essentially suitable for PCR analysis which is one of the key steps in crop improvement programme through marker development and genetic engineering techniques. The yield of DNA ranged from 0.595- 5.550 μg/ml and the purity ratio was between 1.025- 2.010 indicating minimum levels of contaminating metabolites. The present protocol offers as a reliable, and consistent DNA isolation method for chickpea that yields large amount of pure&intact DNA.

Keywords


DNA Isolation, Cicer arietinum, PCR Analysis

References





DOI: https://doi.org/10.17485/ijst%2F2010%2Fv3i12%2F29865