Indian Journal of Science and Technology

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Cytotoxic Effect of Diphtheria Toxin in Mammalian Cell Lines


Affiliations
1 Pasteur Institute of India, Coonoor, Nilgiris-643 103, TN, India
 

Diphtheria toxin is an exotoxin secreted by Corynebacterium diphtheriae, the bacterial pathogen that causes diphtheria. The present study was focused to find out the toxicological effect of diphtheria toxin in guinea pigs and cell lines like Vero, MA104, HeLa, Murine neuroblastoma-2A, BHK-21, Rhabdomyosarcoma and L20B. Animals inoculated with the toxin at different concentrations showed erythema around the site of inoculation after 48 h. When susceptible cell lines inoculated with similar concentration of toxin showed similar morphological changes with certain minor differences were observed. Diphtheria toxin has the capacity to inhibit protein synthesis in mammalian cell lines and thus causing cell death. The study concluded that Vero, MA104 and Rhabdomyosarcoma cell lines could be used as an alternative to in vivo assay for the toxicology study of diphtheria toxin and enables us free from ethical issues.

Keywords

Diphtheria Toxin, Cell Lines, Erythema, Guinea Pigs
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  • Cytotoxic Effect of Diphtheria Toxin in Mammalian Cell Lines

Abstract Views: 535  |  PDF Views: 200

Authors

N. Sivananda
Pasteur Institute of India, Coonoor, Nilgiris-643 103, TN, India
S. Ajesh
Pasteur Institute of India, Coonoor, Nilgiris-643 103, TN, India
C. Manju
Pasteur Institute of India, Coonoor, Nilgiris-643 103, TN, India
P. Sugapriya
Pasteur Institute of India, Coonoor, Nilgiris-643 103, TN, India
M. G. Sudharshan
Pasteur Institute of India, Coonoor, Nilgiris-643 103, TN, India
G. Maheswari
Pasteur Institute of India, Coonoor, Nilgiris-643 103, TN, India

Abstract


Diphtheria toxin is an exotoxin secreted by Corynebacterium diphtheriae, the bacterial pathogen that causes diphtheria. The present study was focused to find out the toxicological effect of diphtheria toxin in guinea pigs and cell lines like Vero, MA104, HeLa, Murine neuroblastoma-2A, BHK-21, Rhabdomyosarcoma and L20B. Animals inoculated with the toxin at different concentrations showed erythema around the site of inoculation after 48 h. When susceptible cell lines inoculated with similar concentration of toxin showed similar morphological changes with certain minor differences were observed. Diphtheria toxin has the capacity to inhibit protein synthesis in mammalian cell lines and thus causing cell death. The study concluded that Vero, MA104 and Rhabdomyosarcoma cell lines could be used as an alternative to in vivo assay for the toxicology study of diphtheria toxin and enables us free from ethical issues.

Keywords


Diphtheria Toxin, Cell Lines, Erythema, Guinea Pigs

References





DOI: https://doi.org/10.17485/ijst%2F2010%2Fv3i12%2F29855