Indian Journal of Science and Technology

Open Access Open Access  Restricted Access Subscription Access

Evaluation of Antioxidant Potential and Antibacterial Activity of Calotropis gigantea and Vinca rosea Using In Vitro Model


Affiliations
1 PG & Research Dept. of Chemistry, Auxilium College, Vellore-632 006, TN, India
 

The different parts of Calotropis gigantea and Vinca rosea belonging to the families of Asclepiadaceae and Apocynaceae were studied for their antioxidant and antimicrobial activities against selected bacterial strains. From the results it was evident that the flower of Vinca rosea showed the highest antioxidant activity of 97.44% at 800 µg which was higher than the standard L-ascorbic acid (94%) and Calotropis gigantea showed the least. Calotropis gigantea exhibited highest inhibition zone of 14, 14 and 11 mm against Escherichia coli, Salmonella typhi and Shigella sonnei when compared to Vinca rosea. Hence, the present study supports the view, that these medicinal plants might be useful as antioxidant and antimicrobial agents.

Keywords

DPPH Assay, Antioxidant, Antimicrobial, Vinca rosea, Bacterial Strains
User

  • Cai YZ, Sun M, Xing J, Luo Q and Corke H (2006) Structure–radical scavenging activity relationships of phenolic compounds from traditional Chinese medicinal plants. Life Sci. 78, 2872–2888.

  • Cakir A, Maci A, Yyldyrym A, Durub ME, Harmandar M and Kazaz V (2003) Isolation and characterization of antioxidant phenolic compounds from the aerial parts of Hypericum hyssopifolium L. J. Ethnopharmacol. 87, 73-83.

  • Carew DP and Patterson BD (1970) The effect of antibiotics on the growth of catharanthus roseus tissue culture. Lloydia. 33, 275–277.

  • Estevinho L, Pereira AP, Moreira L and Luis G (2008) Antioxidant and antimicrobial effects of phenolic compounds extracts of northeast Portugal honey. Food Chemical Toxicol. 49, 3774-3779.

  • Eyob S, Martinsen BK, Tsegaye A, Appelgren M and Skrede G (2008) Antioxidant and antimicrobial activities of extract and essential oil of korarimo. Afri. J. Biotechnol. 7(15), 2585- 2592.

  • Fransworth NR, Svoboda GH and Blomster RN (1968) Antiviral activity of selected catharanthus alkaloids. J. Pharmacol. Sci. 57, 2174–2175.

  • Halliwell B (1994) Free radicals, antioxidants and human disease: curiosity, cause or consequence. Lancer. 344, 721-724.

  • Jaleel CA, Monivannan P and Sankar P (2007) Introduction of drought stress tolerance by ketoconazole in catharanthus roseus is mediated by enhanced antioxidant potential and secondary metabolite accumulation. Colloids Surfaces. B. Biointerfaces. 60, 201-206.

  • Liu YW, Han CH, Lee MH, Hsu FL and Hou WC (2003) Patatin, the tuber storage protein of potato (Solanum tuberosum L.), exhibits antioxidant activity invitro. J. Agric. Food. Chem. 51, 4389-4393.

  • Pathak AK and Argal A (2007) Analgesic activity of Calothropis gigantea flower. Fitoterapia. 78, 40-42.

  • Ram VJ and Kumarin S (2001) Natural products of plant origin as anticancer agents. Drug News Perspect, 8, 465- 482.

  • Sabir SM and Rocha JBT (2008) Antioxidant and hepatoprotective activity of aqueous extract of Solanum fastigiatum (false” Jurubebe”) against paracetamolinduced liver demage in mice. J. Ethnopharmacol. 120, 226-232.

  • Taechowisan T, Chanaphat N, Wanbenjob A and Shen Y (2009) Cytoprotective activity of chemical constituents isolated from Streptomyces sp., Int. J. Biol. Chem. 3(1), 11-17.

  • Uma Devi P, Murugan S, Suja S, Selvi S, Chinnasamy P and Vijayaanad E (2007) Antibacterial, invitro lipid per-oxidation and phytochemical observation on Achyranthes bidentata blume. Pak. J. Nutrition. 6(5), 447-451.

  • Villano D, Pachon MSF, Moya ML, Troncoso AM and Garcia Parrilla MC (2007) Radical scavenging ability of polyphenolic compounds towards DPPH free radical. Talanta. 71, 230-235.

  • Willcox JK, Ash SL and Catignani GL (2004) Antioxidant and prevention of chronic disease. Crit. Rev. Food Sci. Nutrition. 44, 275-295.

  • Young IS and Woodside JV (2001) Antioxidants in health and disease. J. Clinical Pathol. 54, 176-186.


Abstract Views: 873

PDF Views: 215




  • Evaluation of Antioxidant Potential and Antibacterial Activity of Calotropis gigantea and Vinca rosea Using In Vitro Model

Abstract Views: 873  |  PDF Views: 215

Authors

D. Jayakumar
PG & Research Dept. of Chemistry, Auxilium College, Vellore-632 006, TN, India
S. Jhancy Mary
PG & Research Dept. of Chemistry, Auxilium College, Vellore-632 006, TN, India
R. Jaya Santhi
PG & Research Dept. of Chemistry, Auxilium College, Vellore-632 006, TN, India

Abstract


The different parts of Calotropis gigantea and Vinca rosea belonging to the families of Asclepiadaceae and Apocynaceae were studied for their antioxidant and antimicrobial activities against selected bacterial strains. From the results it was evident that the flower of Vinca rosea showed the highest antioxidant activity of 97.44% at 800 µg which was higher than the standard L-ascorbic acid (94%) and Calotropis gigantea showed the least. Calotropis gigantea exhibited highest inhibition zone of 14, 14 and 11 mm against Escherichia coli, Salmonella typhi and Shigella sonnei when compared to Vinca rosea. Hence, the present study supports the view, that these medicinal plants might be useful as antioxidant and antimicrobial agents.

Keywords


DPPH Assay, Antioxidant, Antimicrobial, Vinca rosea, Bacterial Strains

References





DOI: https://doi.org/10.17485/ijst%2F2010%2Fv3i7%2F29801