Indian Journal of Science and Technology

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Trends in Wheat Germ Cell Free Protein Expression System with an Emphasis on Up-scaling and Industrial Application


Affiliations
1 Dept. of Biotechnology, Sreenidhi Instt. of Science and Technol., Yamnampet, Ghatkesar, Hyderabad, India
2 Dept. of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India
3 Laboratory of Gene Regulation and Development, National Instt. of Child Health and Human Development, National Instt. of Health, Bethesda, MD 20892, India
4 School of Chemistry, University of Hyderabad, Hyderabad, India
5 Cell Signaling and Tumor Angiogenesis Lab, Dept. of Genetics, Boys Town National Research Hospital, Omaha, NE, United States
 

Cell-free translation systems have developed significantly over the last 2 decades and improvements in yield have resulted in their use for in vitro protein production in the laboratory. Such cell free systems enable the expression of cytotoxic, regulatory or unstable proteins that cannot be expressed in living cells. We compare the uses of different cell-free translation systems and review recent findings that support the possibility of scaling up and thereby enhancing the industrial application of wheat germ cell free protein expression.

Keywords

Cell Free Biology, in vitro Protein Expression, Scale up of Translation, Wheat Germ Extract
User

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  • Trends in Wheat Germ Cell Free Protein Expression System with an Emphasis on Up-scaling and Industrial Application

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Authors

K. Bhanu Revathi
Dept. of Biotechnology, Sreenidhi Instt. of Science and Technol., Yamnampet, Ghatkesar, Hyderabad, India
V. A. Ramaiah
Dept. of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India
Alan G. Hinnebusch
Laboratory of Gene Regulation and Development, National Instt. of Child Health and Human Development, National Instt. of Health, Bethesda, MD 20892, India
Abani K. Bhuyan
School of Chemistry, University of Hyderabad, Hyderabad, India
K. S. R. Sivasai
Dept. of Biotechnology, Sreenidhi Instt. of Science and Technol., Yamnampet, Ghatkesar, Hyderabad, India
Akulapalli Sudhakar
Cell Signaling and Tumor Angiogenesis Lab, Dept. of Genetics, Boys Town National Research Hospital, Omaha, NE, United States
Laxminarayana Burela
Dept. of Biotechnology, Sreenidhi Instt. of Science and Technol., Yamnampet, Ghatkesar, Hyderabad, India

Abstract


Cell-free translation systems have developed significantly over the last 2 decades and improvements in yield have resulted in their use for in vitro protein production in the laboratory. Such cell free systems enable the expression of cytotoxic, regulatory or unstable proteins that cannot be expressed in living cells. We compare the uses of different cell-free translation systems and review recent findings that support the possibility of scaling up and thereby enhancing the industrial application of wheat germ cell free protein expression.

Keywords


Cell Free Biology, in vitro Protein Expression, Scale up of Translation, Wheat Germ Extract

References





DOI: https://doi.org/10.17485/ijst%2F2010%2Fv3i3%2F29716