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DNA Damage by Sodium Arsenite in Experimental Rats: Ameliorative Effects of Antioxidant Vitamins C and E


Affiliations
1 Dept. Of Plant biology & Plant Biotechnol., RKM. Vivekananda College, Mylapore, Chennai 600 004, India
2 Mayo Clinic, Rochester, Minnesota, United States
 

Serious health problems in humans are caused by arsenic-exposure, which is wide spread in the environment. Sodium arsenite, capable of inducing macromolecular damage is evaluated for its DNA damaging effect in the blood, liver, kidney and bone marrow cells of Wistar rats. Also the ameliorative potential of a-tocopherol (400 mg/kg body weight) and ascorbic acid (200 mg/kg body weight) supplemented orally to arsenic-intoxicated rats (100 ppm in drinking water for thirty days) in neutralizing the genotoxic effect of arsenite was explored. Detection of DNA damage at cellular level is evaluated by single cell gel electrophoresis or comet assay, under alkaline conditions. This study demonstrated that dietary supplementation of antioxidants such as vitamins C and E could ameliorate arsenite induced toxicity in experimental rats.

Keywords

Arsenic, Antioxidants, Ascorbic Acid, Comet Assay, DNA Damage, Genotoxicity, in vivo, α- Tocopherol
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  • DNA Damage by Sodium Arsenite in Experimental Rats: Ameliorative Effects of Antioxidant Vitamins C and E

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Authors

B. S. Balakumar
Dept. Of Plant biology & Plant Biotechnol., RKM. Vivekananda College, Mylapore, Chennai 600 004, India
K. Ramanathan
Mayo Clinic, Rochester, Minnesota, United States
S. Kumaresan
Dept. Of Plant biology & Plant Biotechnol., RKM. Vivekananda College, Mylapore, Chennai 600 004, India
R. Suresh
Dept. Of Plant biology & Plant Biotechnol., RKM. Vivekananda College, Mylapore, Chennai 600 004, India

Abstract


Serious health problems in humans are caused by arsenic-exposure, which is wide spread in the environment. Sodium arsenite, capable of inducing macromolecular damage is evaluated for its DNA damaging effect in the blood, liver, kidney and bone marrow cells of Wistar rats. Also the ameliorative potential of a-tocopherol (400 mg/kg body weight) and ascorbic acid (200 mg/kg body weight) supplemented orally to arsenic-intoxicated rats (100 ppm in drinking water for thirty days) in neutralizing the genotoxic effect of arsenite was explored. Detection of DNA damage at cellular level is evaluated by single cell gel electrophoresis or comet assay, under alkaline conditions. This study demonstrated that dietary supplementation of antioxidants such as vitamins C and E could ameliorate arsenite induced toxicity in experimental rats.

Keywords


Arsenic, Antioxidants, Ascorbic Acid, Comet Assay, DNA Damage, Genotoxicity, in vivo, α- Tocopherol

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DOI: https://doi.org/10.17485/ijst%2F2010%2Fv3i3%2F29708