Indian Journal of Science and Technology

Open Access Open Access  Restricted Access Subscription Access

Antibacterial Activity of Crude Extracts of Marine Invertebrate Polyclinum madrasensis Sebastian


Affiliations
1 Dept. of Pharmaceutical Biotechnology, Ultra College of Pharmacy, 4/235, Thasildhar nagar, Madurai-625020, India
2 Dept. of Pharmacology, Ultra College of Pharmacy, Madurai-625020, India
3 Dept. of Pharmaceutics, Ultra College of Pharmacy, Madurai-625020, India
4 1Dept. of Pharmaceutical Biotechnology, Ultra College of Pharmacy, 4/235, Thasildhar nagar, Madurai-625020, India
 

Antibacterial activity of methanol, hexane and petroleum ether extracts of Polyclinum madrasensis was evaluated by cup plate method against various clinical isolates. Two different concentrations (1&4 mg/ml) were analyzed. Methanolic extract of P. madrasensis demonstrated high degree of activity against all tested bacterial isolates in increasing concentrations, whereas hexane extract showed good activity against gram negative pathogens in high concentration and moderate against gram positive pathogens in lower concentration. All strains were resistant to Pet ether extract in both the concentrations when compared to the standard (ciprofloxacin) used. The MIC and MBC for methanolic extract tested in study inferred that the values range between 0.70-0.95 mg/ml and 0.85-1.1 mg/ml respectively. It was concluded from the study that methanolic extract of P. madrasensis exhibits potential antibacterial property.

Keywords

Antimicrobial Activity, Polyclinum Madrasensis, Cup Plate Method, Clinical Isolates
User

  • Alade PI and Irobi ON (1993) Antifungal activities of crude leaf extract of Acalypha wilkesiana. J. Ethnopharmacol. 39, 171–174.

  • Azumi K, Yoshimizu M, Suzuki S, Ezura Y and Yokosawa H (1990) Inhibitory effect of halocyamine, an antimicrobial substance from ascidian hemocytes, on the growth of fish viruses and marine bacteria. Cell. Mol. Life Sci. 46(10), 1066-1068.

  • Bergquist PR and Bedford JJ (1978) The incidence of antibacterial activity in marine demospongiae; Systematic and geographic considerations. Marine Biol. 46 (3), 215-221.

  • Biard JF, Guyout S, Roassaki C and Verbist JF (1994) Lepadi- formine, a new marine cytotoxic alkaloid from Clavelina lepadiformis. Tetrahedran Lett., 35, 2691-2694.

  • Carte BK (1996) Biomedical potential of marine natural products. Biosci. 46, 271-286.

  • Collins CH, Lyne PM, Grange JM (1995) Microbiological methods. 7th edn. Butterwort – Heinemann Ltd. Britain. pp175–190.

  • Davidson BS (1993) Ascidians: producers of amino acid derived metabolites. Chem. Rev. 93, 1771-1791.

  • Hugo WB, Russell AB (2004) Pharmaceutical microbiology. 7th edn. London: Black Well Scientific Publication. pp196-201.

  • Kurosoki F and Nishi A (1983) Isolation and antimicrobial activity of the phytoalexin–6-methoxymellein from cultured carrot cells. Phytochem. 22(3), 669-672.

  • Seleghim MHR, Lira SP, Kossuga MH, Batista T, Berlinck RGS and Hajdu E (2007) Antibiotic, cytotoxic and enzyme inhibitory activity of crude extracts from Brazilian marine invertebrates. Brazilian J. Pharmacognosy. 17(3), 287-318.

  • Proksch P, Edrada RA and Ebel R (2002) Drugs from the sea-current status and microbiological Implications. Appl. Microbiol. Biotechnol. 59,125-134.

  • Russo GL, Ciarcia G, Presidente E, Siciliano RA and Tosti E (2008) Cytotoxic and apoptogenic activity of a methanolic extract from the marine invertebrate Ciona intestinalis on malignant cell lines. Med. Chem. 4(2), 106-109.


Abstract Views: 424

PDF Views: 136




  • Antibacterial Activity of Crude Extracts of Marine Invertebrate Polyclinum madrasensis Sebastian

Abstract Views: 424  |  PDF Views: 136

Authors

K. Natarajan
Dept. of Pharmaceutical Biotechnology, Ultra College of Pharmacy, 4/235, Thasildhar nagar, Madurai-625020, India
R. Sathish
Dept. of Pharmacology, Ultra College of Pharmacy, Madurai-625020, India
T. Regupathi
Dept. of Pharmaceutics, Ultra College of Pharmacy, Madurai-625020, India
A. Riyaz
1Dept. of Pharmaceutical Biotechnology, Ultra College of Pharmacy, 4/235, Thasildhar nagar, Madurai-625020, India

Abstract


Antibacterial activity of methanol, hexane and petroleum ether extracts of Polyclinum madrasensis was evaluated by cup plate method against various clinical isolates. Two different concentrations (1&4 mg/ml) were analyzed. Methanolic extract of P. madrasensis demonstrated high degree of activity against all tested bacterial isolates in increasing concentrations, whereas hexane extract showed good activity against gram negative pathogens in high concentration and moderate against gram positive pathogens in lower concentration. All strains were resistant to Pet ether extract in both the concentrations when compared to the standard (ciprofloxacin) used. The MIC and MBC for methanolic extract tested in study inferred that the values range between 0.70-0.95 mg/ml and 0.85-1.1 mg/ml respectively. It was concluded from the study that methanolic extract of P. madrasensis exhibits potential antibacterial property.

Keywords


Antimicrobial Activity, Polyclinum Madrasensis, Cup Plate Method, Clinical Isolates

References





DOI: https://doi.org/10.17485/ijst%2F2010%2Fv3i3%2F29703