Indian Journal of Science and Technology

Open Access Open Access  Restricted Access Subscription Access

Genetic Diversity in Azima tetracantha (lam) Assessed through RAPDAnalysis


Affiliations
1 Dept. of Biotechnology, Alpha Arts & Science College, Chennai- 600 116, India
2 Dept. of Biotechnology, Mother Teresa Women’s University, Kodaikanal – 624 102, India
3 Dept. of Botany, Pachiayappa’s College, Chennai – 600 030, India
 

Azima tetracantha is a shrub known for its various therapeutic properties. Genetic diversity was analysed using RAPD primers among six accessions collected from different locations of Tamilnadu, India. Genetic distances were calculated using Nei's coefficient. Dendrogram was constructed on the basis of the similarity matrix data by unweighted pair group method with average (UPGMA) cluster analysis. The analysis with RAPD markers revealed wide variation within A. tetracantha that reflected a high level of diversity within this species.

Keywords

Azima tetracantha, Genetic Diversity, RAPD
User

  • Alam MA, Pallavi Gulati, Aswini KG, Gyan PM and Pradeep KN (2009) Assessment of genetic diversity among Podophyllum hexandrum genotypes of northwestern Himalayan region for Podophyllotoxin production. Indian J. Biotechology. 8, 391-399.

  • Becerra Velasques VB and Gepts P (1994) RFLP diversity in common bean (Phaseolus vulgaris L.). Genome 37, 256-263.

  • Chrubasik S, Pittler M and Roufogalis BD (2005) Zingiberis hizoma: a comprehensive review on the ginger effect and efficacy profiles. Phytomedicine. 12, 684–701.

  • Comes HP and Kadereit JW (1998) The effect of quaternary climatic changes on plant distribution and evolution. Trends Plant Sci. 3. (11), 432-438.

  • Doyle JJ and Doyle JJ (1990) Isolation of plant DNA from fresh tissue. Focus. 12, 13-15.

  • Hebbar SS, Harsha VH and Shripathi GR (2004) Hegde ethnomedicine of Dharwad district in Karnataka, India-plants used in oral health care. J. Ethnopharmacol. 94, 261–266.

  • Hedrick PW (2001) Conservation genetics: where are we now? Trends Ecol. Evoult.16, 629–636.

  • Heun M, Murphy JP and Phillips TD (1994) A comparison of RAPD and isozyme analysis for determining the genetic relationships among Avena sterlis L. accessions. Theor. Appl. Genet. 87, 689-696.

  • Ignacimuthu S, Ayyanar M and Sankarasivaraman K (2008) Ethnobotanical study of medicinal plants used by Paliyar tribals in Theni district of Tamil Nadu, India. Fitoterapia. 79(8), 562-568.

  • Ismail TS, Gopalakrishnan S, Begum VH and Elango V (1997) Anti-inflammatory activity of Salacia oblongaWall. and Azima tetracantha Lam. J. Ethnopharmacol. 56(2),145-152.

  • Jain A, Bhatia S, Banga SS, Prakash CS and Lakshmi Kumaran (1994) Potential use of random amplified polymorphic DNA (RAPD) technique to study the genetic diversity in Indian mustard (Brassica juncea) and its relationship to heterosis. Theor. Appl. Genet. 88, 116-122.

  • Jaswanth A, Begum VH, Akilandeswari S, Begum TN, Manimaran S and Ruckmani K (2001) Effects of Azima tetracantha on dermal wound healing in rats. Hamdard Medicus. 44(3), 13–16.

  • Jin-Ming Chen, Fan Liu , Qing- Feng Wang and Timothy J Motley (2008) Phylogeography of a marsh herb Sagittaria trifolia (Alismataceae) in China inferred from cpDNA atpB–rbcL intergenic spacers. Mol. Phylogenetics & Evol. 48, 168–175.

  • Kirtikar KR and Basu BD (1984) Indian medicinal plants. Vol.1 & 2. 2nd Ed. Bishennd Singh and Mahendra Pal Singh(eds.), Dehra Dun, 54, 582-584.

  • Mehetre SS, Gomes M and Eapen S (2004) RAPD analysis of hybrid nature of the offspring of Gossypium hirsutum x G. raimondii. Curr. Sci. 84, 24-28.

  • Mohamed AF, Wurster M, Schröder G and Lindequist U (2007). Antioxidant, antimicrobial and cytotoxic activities of selected medicinal plants from Yemen. J. Ethnopharmacol. 111(3), 657-666.

  • Nandgude TD, Bhojwani AP, and Krishna Kinage (2007). Analgesic activity of various extracts of Azima tetracantha (Lam). I. J. Green Pharmacy. 1(1) 37-38.

  • Nei M and Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. of the Natl. Acad. of Sci. USA. 76, 5269-5273.

  • Rodriguez JM, Berke T, Engle L and Nienhuis J (1999). Variation among and within Capsicum species revealed by RAPD markers. Theor. Appl. Genet. 99, 147-156.

  • Sarwat M, Das ES and Srivastava EPS (2008) Analysis of genetic diversity through AFLP, SAMPL, ISSR and RAPD markers in Tribulus terrestris, a medicinal herb. Plant Cell Rep. 27, 519–528.

  • Sayed M. Zain Hasan, Mohammed Shafie B. Shafie and Ramisah M. Shah (2009) Analysis of random amplified polymorphic DNA (RAPD) of Artemisia capillaries (Worm wood capillary) in east coast of Penisular Malaysia. World Appl.Sci. J. 6(7), 976-986.

  • Schaal BA, Hayworth DA, Olsen KM, Rauscher JT and Smith WA (1998) Phylogeographic studies in plants: problems and prospects. Mol. Ecol. 7, 465-474.

  • Slatkin M (1987) Gene flow and the geographic structure of populations. Science. 236, 787-792.

  • Sumer Aras, Ahmet Duran and Gulay Yenilmez (2003). Isolation of DNA for RAPD Analysis from Dry Leaf Material of Some Hesperis L. Specimens. Plant Mol. Biol. Reporter. 21, 461a-461f.

  • Torre A, Lopez S, Yglesias E and Cornelius JP (2008) Genetic (AFLP) diversity of nine Cedrela odorata populations in Madre de Dios, southern Peruvian Amazon.Forest Ecol. Manag. 255, 334–339.

  • Wang X.P, Zou Y.P, and Zhang D.M (1996) Problems in the use of RAPD to the study of genetic diversity and systematis. Acta Botanica Sinica. 38(12), 954-962.

  • Wang W, Chen L, Yang P, Hou L, He C, Gu Z and Liu Z (2007) Assessing genetic diversity of populations of top mouth culter (Culter alburnus) in China using AFLP markers. Biochem. Syst. Ecol. 35, 662–669.

  • Williams JGK, Kubelik AR, Livak KJ, Rafalski JA and Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18, 6531-6535.

  • Xie JT, Mchendale S and Yuan CS (2005) Ginseng and diabetes. Am. J. Chin. Med. 33, 397–404

  • Xu H, Lawson D, Kras A and Ryan D (2005) The use of preventive strategies for bone loss. Am. J. Chin. Med. 33, 299–306.

  • Yoo JH, Lee EJ, Kwak CK, Sohn EH, Koh BH, Song IB and Lee KS (2005) Clinical trial of herbal formula on weight loss in obese Korean children. Am. J. Chin. Med. 33, 713–722


Abstract Views: 475

PDF Views: 161




  • Genetic Diversity in Azima tetracantha (lam) Assessed through RAPDAnalysis

Abstract Views: 475  |  PDF Views: 161

Authors

B. Thendral Hepsibha
Dept. of Biotechnology, Alpha Arts & Science College, Chennai- 600 116, India
V. Premalakshmi
Dept. of Biotechnology, Mother Teresa Women’s University, Kodaikanal – 624 102, India
T. Sekar
Dept. of Botany, Pachiayappa’s College, Chennai – 600 030, India

Abstract


Azima tetracantha is a shrub known for its various therapeutic properties. Genetic diversity was analysed using RAPD primers among six accessions collected from different locations of Tamilnadu, India. Genetic distances were calculated using Nei's coefficient. Dendrogram was constructed on the basis of the similarity matrix data by unweighted pair group method with average (UPGMA) cluster analysis. The analysis with RAPD markers revealed wide variation within A. tetracantha that reflected a high level of diversity within this species.

Keywords


Azima tetracantha, Genetic Diversity, RAPD

References





DOI: https://doi.org/10.17485/ijst%2F2010%2Fv3i2%2F29672