Indian Journal of Science and Technology

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In vitro Propagation and Cell Suspension Culture of Callistemon citrinus L.


Affiliations
1 Center for Research and Development, PRIST University, Thanjavur, Tamilnadu, India
2 Dept. of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, India
 

The bottle brush tree, Callistemon citrinus is a shrub that is nearing extinction. An attempt to revive this plant and propagate it in vitro has been performed for large scale production. Calli cultured on MS medium with different combinations of cytokinins and auxins viz. 1.0 mg/l Kinetin along with 1.0 mg/l IAA; 1.5 mg/l Kinetin along with 1.0 mg/l IAA; 1.0 mg/l Kinetin along with 1.0 mg/l IBA, and 2.0 mg/l Kinetin along with 1.5 mg/l IBA. All combinations were very effective in inducing callus. 0.5 mg/l kinetin along with 0.5 mg/l IAA was very effective for shoot regeneration and shoot elongation. The developed shoots when transferred to MS medium, supplemented with 1.5mg/l IBA, shows ischolar_main development within a week. Axillary bud proliferation is also observed in the explants inoculated in MS medium supplemented with combinations of kinetin and IAA.

Keywords

Callistemon citrinus, Indole Acetic Acid (IAA), Indole Butyric Acid ((IBA), Benzyl Amino Purine(BAP)
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  • In vitro Propagation and Cell Suspension Culture of Callistemon citrinus L.

Abstract Views: 846  |  PDF Views: 164

Authors

R. S. David Paul Raj
Center for Research and Development, PRIST University, Thanjavur, Tamilnadu, India
Sheena Michael Morais
Dept. of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, India
K. Gopalakrishnan
Center for Research and Development, PRIST University, Thanjavur, Tamilnadu, India

Abstract


The bottle brush tree, Callistemon citrinus is a shrub that is nearing extinction. An attempt to revive this plant and propagate it in vitro has been performed for large scale production. Calli cultured on MS medium with different combinations of cytokinins and auxins viz. 1.0 mg/l Kinetin along with 1.0 mg/l IAA; 1.5 mg/l Kinetin along with 1.0 mg/l IAA; 1.0 mg/l Kinetin along with 1.0 mg/l IBA, and 2.0 mg/l Kinetin along with 1.5 mg/l IBA. All combinations were very effective in inducing callus. 0.5 mg/l kinetin along with 0.5 mg/l IAA was very effective for shoot regeneration and shoot elongation. The developed shoots when transferred to MS medium, supplemented with 1.5mg/l IBA, shows ischolar_main development within a week. Axillary bud proliferation is also observed in the explants inoculated in MS medium supplemented with combinations of kinetin and IAA.

Keywords


Callistemon citrinus, Indole Acetic Acid (IAA), Indole Butyric Acid ((IBA), Benzyl Amino Purine(BAP)

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DOI: https://doi.org/10.17485/ijst%2F2010%2Fv3i1%2F29648