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Free Radical Scavenging Activity and HPTLC Finger Print of Pterocarpus santalinus L. - an in vitro study


Affiliations
1 Department of Biomedical Engineering, Sathyabama University, Chennai – 119, India
2 Jaya College of Engineering, Department of Chemistry, Chennai, India
3 Department of Biochemistry, St. Joseph’s College of arts & Science, Cuddalore – 1, India
4 Department of Chemistry, National Institute of Technology, Tiruchirappalli – 15, India
 

Methanol extract of Pterocarpus santalinus (leaves) was evaluated for HPTLC finger print, phytochemical analysis and antioxidant activity. Preliminary phytochemical screening (HPTLC fingerprint) revealed the presence of terpenoids, steroids, flavonoids and carbohydrates. DPPH assay was used to determine the antioxidant property. The methanol extract of Pterocarpus santalinus showed significant DPPH radical inhibition (83.4% at 25 mg/ml concentration). The study reveals the potency of Pterocarpus santalinus as antioxidant source.

Keywords

Antioxidant, DPPH Assay, Pterocarpus Santalinus, Secondary Metabolites
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  • Chopra RN, Nayar SL and Chopra IC (1956) Glossary of Indian medicinal plants. India, CSIR, p: 171.
  • Cook NC and Samman S (1996) Flavonoids: chemistry, metabolism, cardioprotective effects, and dietary sources. J. Nutr. Biochem. 7, 66-76.
  • Edeogal HO, OKWU DE and Mbaebie Bo (2005) Phytochemical constituents of some Nigerian medicinal plants. African J. Biotechnol. 4, 685-688.
  • Frankel E (1995) Nutritional benefits of flavonoids. International conference on food factors: Chemistry and cancer prevention, Hamamatsu, Japan. Abstracts, C6- 2.
  • Koleva II, Van Beek TA, Linssen JPH, de Gischolar_main A and Evstatieva LN (2002) Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem. Analysis. 13, 8-17.
  • Krishnaveni KS and Srinivasa Rao JV (2000) An isoflavone from Pterocarpus santalinus Phytochem. 53, 605-606.
  • Larson RA (1988) The antioxidant of higher plants. Phytochem. 27, 969-78.
  • Liao K and Yin M (2000) Individual and combined antioxidant effects of seven phenolic agents in human erythrocyte membrane ghosts and phosphatidylcholine liposome systems: importance of the partition coefficient. J. Agric. Food Chem. 48, 2266-2270.
  • Manjunatha BK (2006) Antibacterial activity of Pterocarpus santalinus. Indian J. Pharma. Sci. 68, 115-116.
  • Omisore NOA, Adewunmi CO, Iwalewa EO, Ngadjui BT, Adenowo TK, Abegaz BM, Ojewole JA and Watchueng J (2005) Antitrichomonal and antioxidant activities of Dorstenia barteri and Dorstenia convexa. Brazilian J. Medical Biol. Res. 38, 1087-94.
  • Sanchez-Moreno, Larrauri JA and Saura- Calixto F (1998) A procedure to measure the antiradical efficiency of polyphenols. J. Sci. Food Agric. 76, 270-276.
  • Shoba Narayan, Rethinam Sundresan Devi, Vani Ganapathi and Chennam Srinivasulu Shyamala Devi (2007) Effect of Pterocarpus santalinus Extract on the Gastric Pathology Elicited by a Hypertensive Drug in Wistar Rats. Pharm. Biol. 45: 468 – 474.
  • Srinivas Reddy B, Kiran Kumar Reddy R, Naidu VGM, Madhusudhana, Sachin K, Agwane B, Ramakrishna S, Prakash and Diwan V (2008) Evaluation of antimicrobial, antioxidant and wound healing potentials of Holoptelea integrifolia. J. Ethnopharmacol. 115, 249-256.

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  • Free Radical Scavenging Activity and HPTLC Finger Print of Pterocarpus santalinus L. - an in vitro study

Abstract Views: 519  |  PDF Views: 116

Authors

S. Arokiyaraj
Department of Biomedical Engineering, Sathyabama University, Chennai – 119, India
S. Martin
Jaya College of Engineering, Department of Chemistry, Chennai, India
K. Perinbam
Department of Biomedical Engineering, Sathyabama University, Chennai – 119, India
P. Marie Arockianathan
Department of Biochemistry, St. Joseph’s College of arts & Science, Cuddalore – 1, India
V. Beatrice
Department of Chemistry, National Institute of Technology, Tiruchirappalli – 15, India

Abstract


Methanol extract of Pterocarpus santalinus (leaves) was evaluated for HPTLC finger print, phytochemical analysis and antioxidant activity. Preliminary phytochemical screening (HPTLC fingerprint) revealed the presence of terpenoids, steroids, flavonoids and carbohydrates. DPPH assay was used to determine the antioxidant property. The methanol extract of Pterocarpus santalinus showed significant DPPH radical inhibition (83.4% at 25 mg/ml concentration). The study reveals the potency of Pterocarpus santalinus as antioxidant source.

Keywords


Antioxidant, DPPH Assay, Pterocarpus Santalinus, Secondary Metabolites

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DOI: https://doi.org/10.17485/ijst%2F2008%2Fv1i7%2F29602