Indian Journal of Science and Technology

The PDF file you selected should load here if your Web browser has a PDF reader plug-in installed (for example, a recent version of Adobe Acrobat Reader).

If you would like more information about how to print, save, and work with PDFs, Highwire Press provides a helpful Frequently Asked Questions about PDFs.

Alternatively, you can download the PDF file directly to your computer, from where it can be opened using a PDF reader. To download the PDF, click the Download link above.

Fullscreen Fullscreen Off


An alkaline protease producing strain Aspergillus niger was isolated from local soil samples and enzyme production was optimized under submerged conditions. Maximum enzyme production of the culture occurred in mesophilic temperature 45°C and pH 8.5. Glucose and ammonium sulfate proved to be the best carbon and nitrogen sources respectively. The molecular weight of the enzyme determined by SDS-PAGE was found to be 38 kDa. The enzyme acted optimally at pH 10 and 50°C. It was thermo stable and retained full activity even at the end of 1 hour of incubation at 40°C. It was inhibited by Cu++, Hg++, Zn++, EDTA and sodium azide. The enzyme retained more than 50% activity after 60 min incubation at 40°C in the presence of detergents such as Tide, Surf, Wheel and Henko indicating its suitability for application in detergent industry.

Keywords

Alkaline Protease, Aspergillus niger, Purification, Characterization, Detergent
User