Indian Journal of Science and Technology

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Application of Latin Square Design for the Evaluation and Screening of Supplementary Nitrogen Source for L-asparaginase Production by Aspergillus terreus MTCC 1782


Affiliations
1 Department of Biotechnology, St. Joseph’s College of Engineering, Chennai–600 119, India
2 Department of Chemical Engineering, Alagappa College of Technology, Anna University, Chennai - 600 025, India
 

The effect of ammonium chloride, urea and sodium nitrate as supplementary nitrogen source on the production of extracellular L-asparaginase using Aspergillus terreus MTCC 1782 was investigated using 5- level Latin square design. The statistical reliability and significance of the variables was studied by performing ANOVA for experimental L-asparaginase activity using Dataplot software. Among the supplementary nitrogen sources studied, ammonium chloride was found to be the best for maximum L-asparaginase activity (confidence level of 96.61%) with less biomass formation (confidence level of 21.93%). The maximum L-asparaginase activity obtained was of 26.47 IU/mL by A. terreus MTCC 1782 using groundnut oil cake powder as natural substrate in submerged fermentation.

Keywords

Aspergillus terreus, L-asparaginase, Latin Square Design
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  • Ali SS, Rai V, Soni K, Kulshrestha P and Lai SK (1994) A fungal Lasparaginase with potential antitumor activity. Ind. J. Microbiol. 34, 73–76.

  • Berenbaum MC, Ginsburg H and Gilbert DM (1970) Effects of L-asparaginase on lymphocyte target cell reactions In vitro. Nature. 227, 1147–1148.

  • Box GEP, Hunder WG and Hunder SJ (1978) Statistics for experiments, John Wiley & Sons Inc., New York.

  • Broome JD (1961) Evidence that the L-asparaginase activity of Guinea pig serum is responsible for its antilymphoma effects. Nature. 171, 1114–1115.

  • Broome JD (1965) Antilymphoma activity of Lasparaginase in vivo: Clearance rates of enzyme preparations from Guinea pig serum and yeast in relation to their effect on tumor growth. J. Natl. Cancer Inst. 35, 967–974.

  • Joint FAO/WHO Expert Committee on Food Additives (JECFA) Compendium of Food Additive Specifications (2001) General specifications and considerations for enzyme preparations used in food processing, FAO Food & Nutrition. 52(9), 37–39.

  • Kwak KO, Jung SJ, Chumg SY, Kang CM, Huh YI and Bae S O (2006) Optimization of culture conditions for CO2 fixation by chemoautotrophic microorganism strain YN-1 using factorial design. Biochem. Eng. J. 31, 1–7.

  • Mishra A (2006) Production of L-asparaginase, an anticancer agent, from Aspergillus niger using agricultural waste in solid state fermentation. Appl. Biochem. Biotechnol. 135, 33–42.

  • Pedreschi F, Kaack K and Granby K (2008) The effect of Asparaginase on acrylamide formation in French fries. Food Chem. 109, 386-392.

  • Raha SK, Roy SK, Dey SK and Chakrabarty SL (1990) Purification and properties of an Lasparaginase from Cylindrocarpon obtusisporum MB-10. Biochem. Int. 2, 987–1000.

  • Sarquis MIM, Oliviera EMM, Santos AS and Da- Costa GL (2004) Production of L-asparaginase by filamentous Fungi. Mem. Inst. Oswaldo. Cruz. 99, 489–492.

  • Shaffer PM, Arst HN Jr, Estberg L, Fernando L, Tran Ly and Sitter M (1988) An asparaginase of Aspergillus nidulans is subject to oxygen repression in addition to nitrogen metabolite repression. Mol. Gen. Genet. 212, 337–341.

  • Torbjorn L, Elisabeth S, Lisbeth A, Bernt T, Asa N, Jarle P and Rolf B (1998) Experimental design and optimization. Chemometr. Intell. Lab. 42, 3–40.

  • Wriston JC Jr and Yellin TO (2001) L-asparaginase - a review. Adv. Enz. 39, 185–248.

  • Zheng ZM, Hu QL, Hao J, Xu F, Guo NN, Sun Y and Liu DH (2008) Statistical optimization of culture conditions for 1,3-propanediol by Klebsiella pneumoniae AC 15 via Central Composite Design. Bioresour. Technol. 99, 1052–1056.


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  • Application of Latin Square Design for the Evaluation and Screening of Supplementary Nitrogen Source for L-asparaginase Production by Aspergillus terreus MTCC 1782

Abstract Views: 362  |  PDF Views: 125

Authors

G. Baskar
Department of Biotechnology, St. Joseph’s College of Engineering, Chennai–600 119, India
S. Renganathan
Department of Chemical Engineering, Alagappa College of Technology, Anna University, Chennai - 600 025, India

Abstract


The effect of ammonium chloride, urea and sodium nitrate as supplementary nitrogen source on the production of extracellular L-asparaginase using Aspergillus terreus MTCC 1782 was investigated using 5- level Latin square design. The statistical reliability and significance of the variables was studied by performing ANOVA for experimental L-asparaginase activity using Dataplot software. Among the supplementary nitrogen sources studied, ammonium chloride was found to be the best for maximum L-asparaginase activity (confidence level of 96.61%) with less biomass formation (confidence level of 21.93%). The maximum L-asparaginase activity obtained was of 26.47 IU/mL by A. terreus MTCC 1782 using groundnut oil cake powder as natural substrate in submerged fermentation.

Keywords


Aspergillus terreus, L-asparaginase, Latin Square Design

References





DOI: https://doi.org/10.17485/ijst%2F2009%2Fv2i12%2F29559