Open Access Open Access  Restricted Access Subscription Access

Egg Hatchability and Larvicidal Activity of Swertia chirata Buch. - Hams. ex Wall. against Aedes aegypti L. and Culex quinquefasciatus Say


Affiliations
1 Entomology Research Institute, Loyola College, Chennai-600 034, India
2 Department of Plant Biology and Biotechnology, School of Life Sciences, Loyola College, Chennai-600 034, India
 

Crude Swertia chirata extracts (hexane, ethyl acetate&methanol) were tested for egg hatchability and larvicidal activity against two vector mosquitoes, Aedes aegypti and Culex quinquefasciatus. The lowest egg hatchability was recorded at 1000 ppm of ethyl acetate extract which showed 12.6, 20.4 and 25.4 % at 0-6, 6-12 and 12-18 h respectively against Cx. quinquefasciatus; it was 11.4, 17.8 and 23.4 % at 0-6, 6-12 and 12-18 h respectively against Ae. aegypti. The LC50 values for the larvicidal activity of ethyl acetate extract of S. chirata against first, second, third and fourth instar larvae of Cx. quinquefasciatus were 164.91, 220.10, 284.05 and 326.46 ppm and against Ae. aegypti 192.67, 237.30, 339.06 and 329.29 ppm respectively.

Keywords

Swertia Chirata, Culex Quinquefasciatus, Aedes Aegypti, Egg Hatchability, Larvicidal Activity
User

  • Ananthakrishnan TN (1988) Insect plant interactions problems and perspectives, In: Dynamics of insect plant interaction (Eds. Ananthakrishnan TN & Raman A). New Delhi. Oxford & IBH Publishing Co. pp: 1-11.
  • Bowers WS, Sener B, Evans PH, Bingol F and Erdogan I (1995) Activity of Turkish medicinal plants against mosquitoes Aedes aegypti aind Anopheles gambiae. Insect Sci. & Appl. 16 (3&4): 330-342.
  • Cetin H, Erler F and Yanikoglu A (2004) Larvicidal activity of a botanical natural product, AkseBio2, against Culex pipiens. Fitoterapia. 75, 724-728.
  • Hendarto SK and Hadinegoro SR (1992) Dengue encephalopathy. Acta. Paediatr. Jpn. 34, 350–357.
  • Jang YS, Kim MK, Ahn YJ and Lee HS (2002) Larvicidal activity of Brazilian plants against Aedes aegypti and Culex pipiens pallens (Diptera:Culicidae). Agric. Chem. Biotechnol. 45(3), 131-134.
  • Jensen SR and Schripsema J (2002) Gentainaceae systematics and natural History (Eds. Struwe L & Albert V), Cambridge University Press. London. pp: 573-632.
  • Joshi P and Dhawan V (2005) Swertia chirayita – an overview. Cur. Sci. 89, 635-640.
  • Kirtikar KR, and Basu BD (1984) Indian medicinal plants. (Lalit Mohan Basu, 49, Leader Road, Alahabad 3). pp: 1664–1666.
  • Lahlou MR, Berrada M, Hmamouchi and Lyagoubi M (2001) Effect of some Moroccan medicinal plants on mosquito larvae. Thérapie. 56, 193-196.
  • Maheswaran R, Sathish S and Ignacimuthu S (2008) Larvicidal activity of Leucas aspera (Willd.) against the larvae of Culex quinquefasciatus Say. and Aedes aegypti L. Int. J. Integrative Biol. 2 (3), 214 – 217.
  • Miura T, Schafer CH, Takahashi RM and Mulligan FS (1976) Effects of insect growth inhibitor, Dimilin, on hatching of mosquito eggs. J. Econ. Ent. 69, 655 –658.
  • Muthukrishnan J, Karlheinz S, Klaus HH and Matthias WL (1999) Inhibition of juvenile biosynthesis in Gryllus bimaculatus by Glycosmis pentaphylla leaf compounds. Phytochem. 50, 249-254.
  • Ouda NAA, A1-chalabi BBM, A1- charchafchi FFMR, Mohsen ZZH (1998) Extract of Atriplex canescens against Culex quinquefasciatus. Pharm. Biol. 36(1), 69–71.
  • Pancharoen C, Kulwichit W, Tantawichien T, Thisyakorn U and Thisyakorn C (2002) Dengue infection: a global concern. J. Med. Assoc. Thai. 85, S25-33.
  • Pitasawat B, Champakaew D, Choochote W, Jitpakdi A, Chaithong U, Kanjanapothi D, Rattanachanpichai E, Tippawangkosol P, Riyong D, Tuetun B and Chaiyasit D (2007) Aromatic plant-derived essential oil: An alternative larvicide for mosquito control. Fitoterapia. 78, 205-210.
  • Redwane A, Lazrek HB, Bouallam S, Markouk M, Amarouch H and Jana M (2002) Larvicidal activity extracts from Quercus lusitania var. infectoria galls (Oliv.). J. Ethnopharmacol. 79, 261-263.
  • Rembold H (1994) Advances in invertebrate reproduction. Elsevier Sci. Publ. 3, 481-491.
  • Southgate BA (1984) Recent advances in the epidemiology and control of filarial infections including entomological aspects of transmission. Trans. R. Soc. Trop. Med. Hyg., 78, 19-28.
  • Su and Mulla (1998) Ovicidal activity of neem products (Azadirachtin) against Culex tarsalis and Culex quinquefasciatus (Diptera: Culicidae). J. Am. Mosq. Control. Assoc., 14(2), 204–209.
  • Sukumar K, Perich MJ and Booba LR (1991) Botanical derivatives in mosquito control: A Review. J. Am. Mosq. Control Assoc., 7, 210-237.
  • World Health Organization (1975) Instruction for determining the susceptibility or resistance of mosquito larvae to insecticides. Mimeographed document. WHO/VBC/1975, 583.
  • World Health Organization (1997) UNDP/World Bank/ WHO Special Programme for Research and Training in Tropical Diseases (TDR), Tropical Disease Research: Progress 1995-96. Thirteenth Programme Report of the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases, Geneva, Switzerland. pp: 141.
  • World Health Organization (2003) Dengue (Online, access in 03/06/2003), Available at http://w.w.w.who.int/inf-fs/en/fact117.html.
  • Yang YC, Lee SG, Lee HK, Kim MK, Lee SH and Lee HS (2002) A Piperidine amide extracted from Piper longum L. fruit shows activity against Aedes aegypti mosquito larvae. J. Agric. Food Chem. 50, 3765–3767.

Abstract Views: 399

PDF Views: 126




  • Egg Hatchability and Larvicidal Activity of Swertia chirata Buch. - Hams. ex Wall. against Aedes aegypti L. and Culex quinquefasciatus Say

Abstract Views: 399  |  PDF Views: 126

Authors

K. Balaraju
Entomology Research Institute, Loyola College, Chennai-600 034, India
R. Maheswaran
Entomology Research Institute, Loyola College, Chennai-600 034, India
P. Agastian
Department of Plant Biology and Biotechnology, School of Life Sciences, Loyola College, Chennai-600 034, India
S. Ignacimuthu
Entomology Research Institute, Loyola College, Chennai-600 034, India

Abstract


Crude Swertia chirata extracts (hexane, ethyl acetate&methanol) were tested for egg hatchability and larvicidal activity against two vector mosquitoes, Aedes aegypti and Culex quinquefasciatus. The lowest egg hatchability was recorded at 1000 ppm of ethyl acetate extract which showed 12.6, 20.4 and 25.4 % at 0-6, 6-12 and 12-18 h respectively against Cx. quinquefasciatus; it was 11.4, 17.8 and 23.4 % at 0-6, 6-12 and 12-18 h respectively against Ae. aegypti. The LC50 values for the larvicidal activity of ethyl acetate extract of S. chirata against first, second, third and fourth instar larvae of Cx. quinquefasciatus were 164.91, 220.10, 284.05 and 326.46 ppm and against Ae. aegypti 192.67, 237.30, 339.06 and 329.29 ppm respectively.

Keywords


Swertia Chirata, Culex Quinquefasciatus, Aedes Aegypti, Egg Hatchability, Larvicidal Activity

References





DOI: https://doi.org/10.17485/ijst%2F2009%2Fv2i12%2F29558