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Molecular Characterization of Vembur Sheep (Ovis aries) of South India Based on Microsatellites


Affiliations
1 Department of Animal Genetics and Breeding, Madras Veterinary College Tamilnadu Veterinary and Animal Sciences University, Chennai – 600 007, India
 

The Vembur sheep is a mutton breed, adapted to semi arid habitat and distributed in and around Thoothukudi, Virudhunagar and Tirunelveli districts of Tamilnadu, India. Microsatellite characterization yielded a total of 147 alleles in the sampled population for 25 marker loci under investigation, with a mean of 5.88 ± 0.29 alleles per locus. Number of alleles observed on each locus varied between two and nine. The effective number of alleles observed with a mean of 4.0950 ± 0.23 alleles per locus. The mean PIC value was 0.6905 ± 0.02. The frequency of majority of the loci returned highly significant chi-square values suggesting departure from HWE. The observed heterozygosity (Ho) values varied between 0.1333 and 1.0000, while the expected (He) values ranged from 0.4847 to 0.8537. The mean values were 0.5202 ± 0.04 and 0.7339 ± 0.02 respectively. FIS estimates were negative for three loci indicating more heterozygous nature of the population. The mean value observed was 0.2954 ± 0.31.

Keywords

Vembur Sheep, Microsatellites, PIC, FIS, India
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  • Molecular Characterization of Vembur Sheep (Ovis aries) of South India Based on Microsatellites

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Authors

S. Pramod
Department of Animal Genetics and Breeding, Madras Veterinary College Tamilnadu Veterinary and Animal Sciences University, Chennai – 600 007, India
P. Kumarasamy
Department of Animal Genetics and Breeding, Madras Veterinary College Tamilnadu Veterinary and Animal Sciences University, Chennai – 600 007, India
A. Rosalyn Mary Chandra
Department of Animal Genetics and Breeding, Madras Veterinary College Tamilnadu Veterinary and Animal Sciences University, Chennai – 600 007, India
P. Sridevi
Department of Animal Genetics and Breeding, Madras Veterinary College Tamilnadu Veterinary and Animal Sciences University, Chennai – 600 007, India
P. S. Rahumathulla
Department of Animal Genetics and Breeding, Madras Veterinary College Tamilnadu Veterinary and Animal Sciences University, Chennai – 600 007, India

Abstract


The Vembur sheep is a mutton breed, adapted to semi arid habitat and distributed in and around Thoothukudi, Virudhunagar and Tirunelveli districts of Tamilnadu, India. Microsatellite characterization yielded a total of 147 alleles in the sampled population for 25 marker loci under investigation, with a mean of 5.88 ± 0.29 alleles per locus. Number of alleles observed on each locus varied between two and nine. The effective number of alleles observed with a mean of 4.0950 ± 0.23 alleles per locus. The mean PIC value was 0.6905 ± 0.02. The frequency of majority of the loci returned highly significant chi-square values suggesting departure from HWE. The observed heterozygosity (Ho) values varied between 0.1333 and 1.0000, while the expected (He) values ranged from 0.4847 to 0.8537. The mean values were 0.5202 ± 0.04 and 0.7339 ± 0.02 respectively. FIS estimates were negative for three loci indicating more heterozygous nature of the population. The mean value observed was 0.2954 ± 0.31.

Keywords


Vembur Sheep, Microsatellites, PIC, FIS, India

References





DOI: https://doi.org/10.17485/ijst%2F2009%2Fv2i11%2F29538