Indian Journal of Science and Technology

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Sorting out of Interference in Detection of Endotoxins in Biotherapeutic Drugs


Affiliations
1 P.G. Department of Zoology, Jamal Mohamed College (Autonomous), Tiruchirappalli, India
2 P.G. Research Dept. of Zoology & Wildlife Biology, AVC College (Autonomous), Mannampandal-609 305, TN, India
 

The effect of varying interference factors responsible for false positive results was investigated. The interfering factors inhibiting the endotoxins in activated lymphocytes sample was sorted by heat denaturation. These activated lymphocytes are biotherapeutic drugs which are used for autologous immune enhancement therapy. The technique used for denaturation can be applied to protein samples as well, to get rid of inhibition while quantifying endotoxins.

Keywords

LAL, CSE, Bacterial Endotoxin, Endotoxin Limit and Interference
User

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  • Sorting out of Interference in Detection of Endotoxins in Biotherapeutic Drugs

Abstract Views: 379  |  PDF Views: 172

Authors

Ramesh Pennamareddy
P.G. Department of Zoology, Jamal Mohamed College (Autonomous), Tiruchirappalli, India
K. Prabakar
P.G. Department of Zoology, Jamal Mohamed College (Autonomous), Tiruchirappalli, India
J. Pandiyan
P.G. Research Dept. of Zoology & Wildlife Biology, AVC College (Autonomous), Mannampandal-609 305, TN, India

Abstract


The effect of varying interference factors responsible for false positive results was investigated. The interfering factors inhibiting the endotoxins in activated lymphocytes sample was sorted by heat denaturation. These activated lymphocytes are biotherapeutic drugs which are used for autologous immune enhancement therapy. The technique used for denaturation can be applied to protein samples as well, to get rid of inhibition while quantifying endotoxins.

Keywords


LAL, CSE, Bacterial Endotoxin, Endotoxin Limit and Interference

References





DOI: https://doi.org/10.17485/ijst%2F2009%2Fv2i11%2F29529