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Micromass Culture: a Recent in vitro System for Testing Embryotoxic Potential of Chemicals


Affiliations
1 Division of Toxicology, Central Drug Research Institute, Lucknow- 226001, India
 

Drugs and other chemicals must be evaluated for their teratatological potential before releasing them in the market. One of the model test system that is used in screening of embryotoxic chemicals in vitro, is the micromass culture test. The test involves exposing the rat embryo limb cells or cells of central nervous system (CNS) to various test agents for different time intervals and estimating the inhibition of cell differentiation in these cell cultures. The technique can be best used for identifying strong embryotoxic chemicals due to its sensitivity to a number of classes of potent teratogens and insensitivity to non-teratogens.

Keywords

Embryotoxic, in vitro, Micromass, Teratogen, Teratogenicity
User

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  • Micromass Culture: a Recent in vitro System for Testing Embryotoxic Potential of Chemicals

Abstract Views: 521  |  PDF Views: 99

Authors

Poonam Singh
Division of Toxicology, Central Drug Research Institute, Lucknow- 226001, India
Neeraj Sinha
Division of Toxicology, Central Drug Research Institute, Lucknow- 226001, India
R. K. Singh
Division of Toxicology, Central Drug Research Institute, Lucknow- 226001, India

Abstract


Drugs and other chemicals must be evaluated for their teratatological potential before releasing them in the market. One of the model test system that is used in screening of embryotoxic chemicals in vitro, is the micromass culture test. The test involves exposing the rat embryo limb cells or cells of central nervous system (CNS) to various test agents for different time intervals and estimating the inhibition of cell differentiation in these cell cultures. The technique can be best used for identifying strong embryotoxic chemicals due to its sensitivity to a number of classes of potent teratogens and insensitivity to non-teratogens.

Keywords


Embryotoxic, in vitro, Micromass, Teratogen, Teratogenicity

References





DOI: https://doi.org/10.17485/ijst%2F2009%2Fv2i8%2F29510